Título:	Screening and molecular characterization of a lipase metagenome soil-derived.
Autoria:	CORREIA, V. A. B. ALMEIDA, A. S. S. A. QUIRINO, B. F. KRUGER, R. H. NORONHA, E. F.
Afiliação:	V. A. B. CORREIA, UNIVERSIDADE CATÓLICA DE BRASÍLIA; A. S. S. A. ALMEIDA, UNIVERSIDADE CATÓLICA DE BRASÍLIA; BETANIA FERRAZ QUIRINO, CNPAE; R. H. KRUGER, UNIVERSIDADE DE BRASÍLIA; E. F. NORONHA, UNIVERSIDADE DE BRASÍLIA.
Ano de publicação:	2010
Referência:	In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR – SBBq, 39. LATIN AMERICAN SYMPOSIUM ON THE MOLECULAR MECHANISMS OF SKELETAL MINERALIZATION, 1., 2010, Foz do Iguaçu, PR. Resumos... São Paulo: SBBq, 2010.
Páginas:	1 p.
Conteúdo:	The industrial use of enzymes is an alternative to the chemical industrial processes, in that they offer increased reaction specificity, product purity and reduced environmental impact. Therefore, the search for novel biocatalysts is very important to the identification of enzymes that can compete economically with the inexpensive, but pollutants, traditional chemical processes. The metagenomic approach has accelerated the discovery of novel hydrolases with activity and stability in a wide range of conditions (pH, temperature, organic solvents and salinity), making these technique a powerful tool to the identification of enzymes suitable for biotechnological application. ln the present work, a metagenomic library from Cerrado soil was screened for lipases aiming the identification of novel genes with biotechnological interest. Expression screening of the library for lipases, based on the hydrolysis of tributyrin, resulted in the identification of three different positive clones with insert sizes ranging from 6000 to 8000 bp. The lipolytic activity of these clones was confirmed after retransformation in Escherichia coli EPl-300 and repeated screening. The positive clone pLIP3 shown the highest lipolytic activity on agar-plate assay and is under sequencing procedure using primer walking technique. Future studies include the expression and biochemical characterization of this metagenome derived-lipase.
Thesagro:	Lípase
Palavras-chave:	Enzima industrial Metagenoma
Tipo do material:	Resumo em anais e proceedings
Acesso:	openAccess
Aparece nas coleções:	Resumo em anais de congresso (CNPAE)