Title:	Primer development for detection of Phaseolus vulgaris and Olathe transgenic bean.
Authors:	DINON, A. Z. BROD, F. C. A. MELLO, C. S. SILVEIRA, J. C. M. KOLLING, D. J. TREML, D. FARIA, J. C. ARISI, A. C. M.
Affiliation:	A. Z. DINON, UNIVERSIDADE FEDERAL DE SANTA CATARINA; F. C. A. BROD, UNIVERSIDADE FEDERAL DE SANTA CATARINA; C. S. MELLO, UNIVERSIDADE FEDERAL DE SANTA CATARINA; J. C. M. SILVEIRA, UNIVERSIDADE FEDERAL DE SANTA CATARINA; D. J. KOLLING, UNIVERSIDADE FEDERAL DE SANTA CATARINA; D. TREML, UNIVERSIDADE FEDERAL DE SANTA CATARINA; JOSIAS CORREA DE FARIA, CNPAF; A. C. M. ARISI, UNIVERSIDADE FEDERAL DE SANTA CATARINA.
Date Issued:	2011
Citation:	In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2011.
Description:	Several strategies have been employed for genetically engineering resistance to viruses in transgenic plants. The bean golden mosaic virus (BGMV) is responsible for causing the gold mosaic of common bean (Phaseolus vulgaris L.) and causes severe yield losses due to yellow-green mosaic of leaves, stunted growth and distorted pods. Olathe transgenic bean (Olathe 5.1) was successfully produced by EMBRAPA using the RNA interference (RNAi) concept to silence the rep viral gene in common bean to generate transgenic lines with strong resistance to BGMV. In this work, real time PCR (RT-PCR) method with SYBR Green was developed to detect this newly genetically modified (GM) plant.
Thesagro:	Feijão Phaseolus vulgaris Planta transgênica
Type of Material:	Resumo em anais e proceedings
Access:	openAccess
Appears in Collections:	Resumo em anais de congresso (CNPAF)