Título:	Genome-wide BAC-end sequencing of Musa acuminata DH pahang reveals further insights into the genome organization of banana.
Autoria:	ARANGO, R. E. TOGAWA, R. C. CARPENTIER, S. C ROUX, N. HEKKERT, B. L. KEMA, G. H. J. SOUZA JUNIOR, M. T.
Afiliação:	RAFAEL E. ARANGO, UNALMED-CIB; ROBERTO COITI TOGAWA, CENARGEN; SEBASTIEN C. CARPENTIER, K. U. LEUVEN; NICOLAS ROUX, GLOBAL MUSA GENOMICS CONSORTIUM; BAS L. HEKKERT, PLANT RESEARCH INTERNATIONAL; GERT H. J. KEMA, PLANT RESEARCH INTERNATIONAL; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE.
Ano de publicação:	2011
Referência:	Tree Genetics & Genomes, v. 7, 2011.
Conteúdo:	Banana and plantain (Musa spp.) are grown in more than 120 countries in tropical and subtropical regions and constitute an important staple food for millions of people. A Musa acuminata ssp. malaccencis DH Pahang bacterial artificial chromosome (BAC) library (MAMB) was submitted for BAC-end sequencing. MAMB consists of 23,040 clones, with a 140-kbp average insert size, accounting for a five times coverage of the banana genome. A total of 46,080 reads were generated, and 42,750 (92.8%) high-quality sequences were obtained after trimming for vector and quality. Analysis of these data shows a GC content of 41.39%, whereas interspersed repeats comprise 32.3%. The most common repeated sequences found show homology to ribosomal RNA genes, particularly 18S rRNA, while the Ty3/gypsy type monkey retrotransposon is the most common retro element. The sequence data were used to generate a banana-specific repeat library containing 54 new repetitive elements which accounted for 11.86% of the total nucleotides. Simple sequence repeats represent 0.7% of the sequence data and allowed the identification of 2,455 potentially useful marker sites. Functional annotation identified 2,705 sequences that could code for proteins of known function. Microsynteny analysis shows a higher number of co-linear matches to Oryza sativa, in contrast to Arabidopsis thaliana. This database of BAC-end sequences is useful for the assembly of the complete banana genome sequence and is important for identification in functional genomics experiments.
NAL Thesaurus:	Genome Bananas Bacterial artificial chromosomes
Palavras-chave:	Musa acuminata spp malaccencis
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CENARGEN)