Title:	Genetic diversity and mycelial compatibility groups of the plant-pathogenic fungus Sclerotinia sclerotiorum in Brazil.
Authors:	LITHOLDO JÚNIOR, C. G. GOMES, E. V. LOBO JUNIOR, M. NASSER, L. C. B. PETROFEZA, S.
Affiliation:	C. G. LITHOLDO JÚNIOR, UFG; E. V. GOMES, UFG; MURILLO LOBO JUNIOR, CNPAF; LUIZ CARLOS BHERING NASSER; SILVANA PETROFEZA.
Date Issued:	2011
Citation:	Genetics and Molecular Research, v. 10, n. 2, p. 868-877, 2011.
Description:	The genetic variability of 40 Sclerotinia sclerotiorum isolates from various fields widely distributed throughout Brazil and different host crops was analyzed using RAPD markers and mycelial compatibility groupings (MCGs). The isolates were characterized using 16 random primers of the OPERON series, which produced 121 DNA fragments. UPGMA cluster analysis using Jaccard?s genetic distance and MCGs allowed separation of the isolates into three clusters, with similarity indices of 68.2, 61.8, and 61.8%, and five MCGs. The haplotypes obtained with RAPD markers provided very characteristic groupings of S. sclerotiorum isolates according to MCG, but did not show any relationship with geographic origin or host type. Furthermore, analysis of molecular variance demonstrated that 99.1% of the observed variation was a result of genetic differences between individuals; the host culture did not have a significant effect. This is the first report of high level variability of S. sclerotiorum in Brazil based on the study of isolates of wide geographical origin, supported by RAPD markers and MCGs. These results endorse the prevalence of sexual reproduction in tropical and subtropical regions in contrast to clonal reproduction in temperate regions.
Thesagro:	Sclerotinia sclerotiorum Mofo branco
Keywords:	Diversidade genética
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPAF)