Título:	Mapping of AFLP loci linked to tolerance to cowpea golden mosaic virus.
Autoria:	RODRIGUES, M. A. SANTOS, C. A. F. SANTANA, J. R. F.
Afiliação:	M. A. RODRIGUES, Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana, Feira de Santana, BA; CARLOS ANTONIO FERNANDES SANTOS, CPATSA; J. R. F. SANTANA, Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana, Feira de Santana, BA.
Ano de publicação:	2012
Referência:	Genetics and Molecular Research, v. 11, n. 4, p. 3789-3797, aug. 2012.
Conteúdo:	AFLP markers combined with the bulk segregant analysis methodology was used for the identification of molecular markers associated with the cowpea golden mosaic virus (CGMV) resistance gene in 286 F2 cowpea plants derived from the cross IT97K-499-35 x Canapu T16. Segregation data in the F2 population demonstrated that tolerance to CGMV is controlled by a single dominant gene. Among the 196 combinations of AFLP primers tested, which generated approximately 3800 amplicons, three markers linked to the CGMV resistance gene were identified: E.AAC/M.CCC515 at 4.3 cM, E.AGG/M.CTT280 at 14.2 cM and E.AAA/M.CAG352 at 16.8 cM, with 50.4, 24.4, and 28.7 LOD scores, respectively; the former two markers flank the CGMV loci. These markers could be used for the development of ‘sequence characterized amplified region’ type markers or for greater saturation of this region, to increase the precision of assisted selection for the development of cowpea strains tolerant to CGMV.
Thesagro:	Feijão Doença Mosaico Dourado Vírus Vigna Unguiculata
NAL Thesaurus:	Cowpeas
Palavras-chave:	AFLP Análise de bulks segregantes Mapeamento genético Genetic mapping
Digital Object Identifier:	DOI: 10.4238/2012.August.17.12
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPATSA)