Title:	A systematically improved high quality genome and transcriptome of the human blood fluke Schistosoma mansoni.
Authors:	PROTASIO, A. V. TSAI, I. J. BABBAGE, A. NICHOL, S. HUNT, M. ASLETT, M. A. SILVA, N. de VELARDE, G. S. ANDERSON, T. J. C. CLARK, R. C. DAVIDSON, C. DILLON, G. P. HOLROYD, N. E. LOVERDE, P. T. LLOYD, C. MCQUILLAN, J. OLIVEIRA, G. OTTO, T. D. PARKER-MANUEL, S. J. QUAIL, M. A. WILSON, R. A. ZERLOTINI, A. DUNNE, D. W. BERRIMAN, M.
Affiliation:	ANNA V. PROTASIO, Wellcome Trust Sanger Institute ISHENG J. TSAI, Wellcome Trust Sanger Institute ANNE BABBAGE, Wellcome Trust Sanger Institute SARAH NICHOL, Wellcome Trust Sanger Institute MARTIN HUNT, Wellcome Trust Sanger Institute MARTIN A. ASLETT, Wellcome Trust Sanger Institute NISHADI DE SILVA, Wellcome Trust Sanger Institute GILES S. VELARDE, Wellcome Trust Sanger Institute TIM J. C. ANDERSON, Texas Biomedical Research Institute RICHARD C. CLARK, Wellcome Trust Sanger Institute CLAIRE DAVIDSON, Wellcome Trust Sanger Institute GARY P. DILLON, Wellcome Trust Sanger Institute NANCY E. HOLROYD, Wellcome Trust Sanger Institute PHILIP T. LOVERDE, University of Texas Health Science Center CHRISTINE LLOYD, Wellcome Trust Sanger Institute JACQUELLINE MCQUILLAN, Wellcome Trust Sanger Institute GUILHERME OLIVEIRA, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz THOMAS D. OTTO, Wellcome Trust Sanger Institute SOPHIA J. PARKER-MANUEL, University of York MICHAEL A. QUAIL, Wellcome Trust Sanger Institute R. ALAN WILSON, University of York ADHEMAR ZERLOTINI NETO, CNPTIA DAVID W. DUNNE, University of Cambridge MATTHEW BERRIMAN, Wellcome Trust Sanger Institute.
Date Issued:	2012
Citation:	PLOS Neglected Tropical Diseases, v. 6, n. 1, p. 1-13, Jan. 2012.
Description:	Schistosomiasis is one of the most prevalent parasitic diseases, affecting millions of people in developing countries. Amongst the human-infective species, Schistosoma mansoni is also the most commonly used in the laboratory and here we present the systematic improvement of its draft genome. We used Sanger capillary and deep-coverage Illumina sequencing from clonal worms to upgrade the highly fragmented draft 380 Mb genome to one with only 885 scaffolds and more than 81% of the bases organised into chromosomes. We have also used transcriptome sequencing (RNA-seq) from four time points in the parasite?s life cycle to refine gene predictions and profile their expression. More than 45% of predicted genes have been extensively modified and the total number has been reduced from 11,807 to 10,852. Using the new version of the genome, we identified trans-splicing events occurring in at least 11% of genes and identified clear cases where it is used to resolve polycistronic transcripts. We have produced a high-resolution map of temporal changes in expression for 9,535 genes, covering an unprecedented dynamic range for this organism. All of these data have been consolidated into a searchable format within the GeneDB (www.genedb.org) and SchistoDB (www.schistodb.net) databases. With further transcriptional profiling and genome sequencing increasingly accessible, the upgraded genome will form a fundamental dataset to underpin further advances in schistosome research.
NAL Thesaurus:	Schistosoma
DOI:	10.1371/journal.pntd.0001455
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPTIA)