Título:	Molecular epidemiology of Staphylococcus aureus isolates at different sites in the milk producing dairy farms.
Autoria:	SOUZA, V. de FILHO, A. N. MELO, P. de C. FERNANDES, G. M. FERRAUDO, A. S. CONDE, S. de O. FOGAÇA JÚNIOR, F. A.
Afiliação:	VIVIANE DE SOUZA, CNPC; Antonio Nader Filho, Universidade Estadual Paulista (UNESP) - Jaboticabal, SP; Poliana de Castro Melo, UNESP - Jaboticabal, SP; Guilherme Moraes Ferraudo, UNESP - Jaboticabal, SP.; Antônio Sérgio Ferraudo, UNESP - Jaboticabal, SP; Sandra de Oliveira Conde, UNESP - Jaboticabal, SP; Flavio Augusto Fogaça Junior, UNESP - Jaboticabal, SP.
Ano de publicação:	2012
Referência:	Brazilian Journal of Microbiology, São Paulo, v. 43, n. 4, p. 1646-1650, out./dez. 2012.
Conteúdo:	Abstract: The epidemiological relationships between isolated Staphylococcus aureus strains in milk samples of dairy cows, reagent to California Mastitis Test, individual and group milk was demonstrated in different sites of the production fluxogram, in 12 milk-producing farms in the Gameleira region, municipality of Sacramento MG Brazil, so that localization and transmission modes may be identified. Two hundred and forty-four strains out of 446 samples collected at several sites were isolated and bio-chemically characterized as coagulase-positive staphylococcus. Specific chromosome DNA fragment of the species Staphylococcus aureus was amplified to 106 strains and 103 underwent (PFGE). Samples? collection sites with the highest isolation frequency of Staphylococcus aureus strains comprised papillary ostia (31.1%), CMT-reagent cow milk (21.7%), mechanical milking machines? insufflators (21,7%), milk in milk pails (6.6%) and the milk in community bulk tanks (5.6%). Genetic heterogeneity existed among the isolated 103 Staphylococcus aureus strains, since 32 different pulse-types were identified. Pulse-type 1 had the highest similarity among the isolated strains within the different sites of the milk-production fluxogram. Highest occurrence of pulsetype 1 isolates of Staphylococcus aureus strains was reported in samples collected from the papillary ostia (10.6%), followed by milk samples from CMT-reagent dairy cows (5.8%) and mechanical milking machine insufflators (3.8%). The above shows the relevance of these sites in the agents? transmission mechanism within the context of the farms investigated.
Thesagro:	Staphylococcus aureus Mamite Vaca leiteira Doença animal
NAL Thesaurus:	Mastitis Dairy cows Mammary gland diseases Brazil
Palavras-chave:	Brasil Minas Gerais PFGE Bulk tank PCR
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPC)