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  <title>DSpace Communidade: Embrapa Caprinos (CNPC)</title>
  <link rel="alternate" href="https://www.alice.cnptia.embrapa.br/alice/handle/item/11" />
  <subtitle>Embrapa Caprinos (CNPC)</subtitle>
  <id>https://www.alice.cnptia.embrapa.br/alice/handle/item/11</id>
  <updated>2026-08-09T14:45:37Z</updated>
  <dc:date>2026-08-09T14:45:37Z</dc:date>
  <entry>
    <title>Evaluation of the nutritional state and proposal of sufficiency ranges for Megathyrsus maximus ‘BRS Tamani’ using the compositional nutrient diagnosis and boundary line methods.</title>
    <link rel="alternate" href="https://www.alice.cnptia.embrapa.br/alice/handle/doc/1188614" />
    <author>
      <name>MESQUITA, A. M. S. de</name>
    </author>
    <author>
      <name>ROZANE, D. E.</name>
    </author>
    <author>
      <name>POMPEU, R. C. F. F.</name>
    </author>
    <author>
      <name>CÂNDIDO, M. J. D.</name>
    </author>
    <author>
      <name>LOPES, M. N.</name>
    </author>
    <author>
      <name>NATALE, W.</name>
    </author>
    <author>
      <name>CONCEIÇÃO, M. P. da</name>
    </author>
    <author>
      <name>LIMA, J. D.</name>
    </author>
    <author>
      <name>TAVARES, R. de K. O.</name>
    </author>
    <author>
      <name>SOUZA, H. A. de</name>
    </author>
    <id>https://www.alice.cnptia.embrapa.br/alice/handle/doc/1188614</id>
    <updated>2026-07-26T13:56:28Z</updated>
    <published>2026-01-01T00:00:00Z</published>
    <summary type="text">Título: Evaluation of the nutritional state and proposal of sufficiency ranges for Megathyrsus maximus ‘BRS Tamani’ using the compositional nutrient diagnosis and boundary line methods.
Autoria: MESQUITA, A. M. S. de; ROZANE, D. E.; POMPEU, R. C. F. F.; CÂNDIDO, M. J. D.; LOPES, M. N.; NATALE, W.; CONCEIÇÃO, M. P. da; LIMA, J. D.; TAVARES, R. de K. O.; SOUZA, H. A. de
Conteúdo: Abstracts: Brazil has the largest commercial cattle herd in the world, with 197.2 million head in 2023, representing approximately 12 % of the global herd. Cattle raising in Brazil is primarily carried out in pasture grazing systems. In this context, the adoption of well-adapted, high-yielding, and nutritious forage crops is essential for the sustainability of cattle raising, cost reduction, and increased yield efficiency. The cultivar ‘BRS Tamani’, a Megathysus maximus hybrid, stands out for its short plant height, high nutritional value, and resistance to pasture spittlebug and is recommended for well-drained soils of medium to high fertility, typical of the Brazilian Cerrado region. Despite its high potential, information is lacking regarding nutritional standards for the cultivar, which hinders precise fertilization recommendations. This study aimed to establish nutritional sufficiency ranges for BRS Tamani using the Compositional Nutrient Diagnosis (CND) and boundary line methods, aiming to improve nutrient management and promote yield gains. A database was used to compile information from 123 experimental units of BRS Tamani during the period from July to December 2017. Leaf samples were collected at various growth cycles / cuttings of the forage crop, and chemical analyses were performed to determine contents of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), and sulfur (S). The CND method was used to calculate nutritional levels and identify nutrient imbalances. The boundary line method was applied to establish relationships between yield and the levels of each nutrient. The following critical levels were proposed by the CND: N = 20 g kg-1; P = 2.2 g kg-1; K = 20 g kg-1; Ca = 2.3 g kg-1; Mg = 2.8 g kg-1; and S = 1.4 g kg-1. The values obtained by the boundary line method were as follows: N = 23 g kg-1; P = 2.3 g kg-1; K = 20 g kg-1; Ca = 2.6 g kg-1; Mg = 2.8 g kg-1; and S = 1.3 g kg-1. Highlights: Suggestion of sufficiency ranges for nutrient diagnosis of Megathyrsus maximus ‘BRS Tamani’. The CND and boundary line methods enable determination of sufficiency ranges for forage crops. Region-specific nutritional standards should be established for forage crops.</summary>
    <dc:date>2026-01-01T00:00:00Z</dc:date>
  </entry>
  <entry>
    <title>Avanços na genética e melhoramento de gramíneas forrageiras do gênero Urochloa por meio de ferramentas moleculares.</title>
    <link rel="alternate" href="https://www.alice.cnptia.embrapa.br/alice/handle/doc/1188316" />
    <author>
      <name>LEÃO, U. S.</name>
    </author>
    <author>
      <name>GIRÃO, M. V. D.</name>
    </author>
    <author>
      <name>BUENO, L. G.</name>
    </author>
    <author>
      <name>SILVA, G. R. da</name>
    </author>
    <author>
      <name>DINIZ, F. M.</name>
    </author>
    <id>https://www.alice.cnptia.embrapa.br/alice/handle/doc/1188316</id>
    <updated>2026-07-19T14:51:50Z</updated>
    <published>2026-01-01T00:00:00Z</published>
    <summary type="text">Título: Avanços na genética e melhoramento de gramíneas forrageiras do gênero Urochloa por meio de ferramentas moleculares.
Autoria: LEÃO, U. S.; GIRÃO, M. V. D.; BUENO, L. G.; SILVA, G. R. da; DINIZ, F. M.
Conteúdo: Resumo: Gramíneas do gênero Urochloa constituem um dos grupos mais empregados na alimentação de animais ruminantes no mundo. O objetivo desta investigação foi realizar uma revisão sistemática reunindo estudos relevantes às temáticas ligadas à genética, melhoramento e marcadores moleculares comumente aplicados nos processos de melhoramento do gênero Urochloa. O pacote R de análise bibliométrica, Bibliometrix, processou os dados obtidos nas buscas. O estudo indicou que o Brasil é o país com maior número de publicações relacionadas ao gênero. Os marcadores moleculares mais utilizados são os microssatélites, RAPDs e SNPs, que foram ideais para a construção de mapas de ligação e estudos de diferenciação e diversidade genética. Ficou evidente a necessidade de ampliação dos estudos genéticos em outras espécies de Urochloa, de forma a facilitar a seleção de caracteres de interesse e o direciona-mento de cruzamentos que garantam a formação de genótipos com maior variabilidade genética para exploração nos programas de melhoramento. Abstract: The grasses of the genus Urochloa (synonym Brachiaria) are utilized extensively for animal grazing in the world. The aim of this study was to carry out a systematic review, bringing together studies related to genetics, breeding and use of molecular markers in Urochloa grasses. The R-package Bibliometrix was used for bibliometric and co-citation analysis in this research. Brazil stood out as the country with the largest number of publications related to the genus. Most used molecular markers were microsatellites, RAPDs and SNPs, which were ideal for building linkage maps and for studies of differentiation and genetic diversity. The need to expand genetic studies in other species of Urochloa became evident; in order to allow the selection of characters of interest and the direction of crosses that guarantee the formation of genotypes with greater genetic variability for exploitation in breeding programs. Resumen: Gramíneas do gênero Urochloa constituem um dos grupos mais empregados na alimentação de animais ruminantes no mundo. O objetivo desta investigação foi realizar uma revisão sistemática reunindo estudos relevantes às temáticas ligadas à genética, melhoramento e marcadores moleculares comumente aplicados nos processos de melhoramento do gênero Urochloa. O pacote R de análise bibliométrica, Bibliometrix, processou os dados obtidos nas buscas. O estudo indicou que o Brasil é o país com maior número de publicações relacionadas ao gênero. Os marcadores moleculares mais utilizados são os microssatélites, RAPDs e SNPs, que foram ideais para a construção de mapas de ligação e estudos de diferenciação e diversidade genética. Ficou evidente a necessidade de ampliação dos estudos genéticos em outras espécies de Urochloa, de forma a facilitar a seleção de caracteres de interesse e o direcionamento de cruzamentos que garantam a formação de genótipos com maior variabilidade genética para exploração nos programas de melhoramento.</summary>
    <dc:date>2026-01-01T00:00:00Z</dc:date>
  </entry>
  <entry>
    <title>Evaluation of propidium monoazide for 16S ribosomal RNA metabarcoding assessment of microbial communities in 60-day ripened raw goat milk cheese.</title>
    <link rel="alternate" href="https://www.alice.cnptia.embrapa.br/alice/handle/doc/1188204" />
    <author>
      <name>FEITOZA, S. N. P.</name>
    </author>
    <author>
      <name>LIMA, L. A. de</name>
    </author>
    <author>
      <name>SARAIVA, C. A. S.</name>
    </author>
    <author>
      <name>DIAS, W. da S.</name>
    </author>
    <author>
      <name>MEDEIROS, A. B. A. de</name>
    </author>
    <author>
      <name>FERNANDES, A. C. de C.</name>
    </author>
    <author>
      <name>HEINEMANN, M. B.</name>
    </author>
    <author>
      <name>SOUZA, F. N. de</name>
    </author>
    <author>
      <name>FELISBERTO, N. R. de O.</name>
    </author>
    <author>
      <name>LEMOS, M. L. P.</name>
    </author>
    <author>
      <name>EGITO, A. S. do</name>
    </author>
    <author>
      <name>OLIVEIRA, C. J. B. de</name>
    </author>
    <id>https://www.alice.cnptia.embrapa.br/alice/handle/doc/1188204</id>
    <updated>2026-07-12T16:47:34Z</updated>
    <published>2026-01-01T00:00:00Z</published>
    <summary type="text">Título: Evaluation of propidium monoazide for 16S ribosomal RNA metabarcoding assessment of microbial communities in 60-day ripened raw goat milk cheese.
Autoria: FEITOZA, S. N. P.; LIMA, L. A. de; SARAIVA, C. A. S.; DIAS, W. da S.; MEDEIROS, A. B. A. de; FERNANDES, A. C. de C.; HEINEMANN, M. B.; SOUZA, F. N. de; FELISBERTO, N. R. de O.; LEMOS, M. L. P.; EGITO, A. S. do; OLIVEIRA, C. J. B. de
Conteúdo: Abstract: Cheese ripening is a complex microbial process marked by significant shifts in microbial composition. Considering that propidium monoazide (PMA) depletes DNA from nonviable cells, we hypothesized that PMA treatment of cheese samples could affect the microbiota characterization of 60-d-ripened raw goat curd cheese by 16S rRNA metabarcoding sequencing. After ripening, PMA-treated and nontreated (control) samples from the same cheese units were processed for DNA extraction, library preparation, and 16S rRNA metabarcoding sequencing on an Illumina MiSeq platform. Downstream bioinformatic analyses for microbial diversity assessment were performed using QIIME 2 and the phyloseq package in R. Statistical analyses included permutational multivariate analysis of variance (PERMANOVA), Wilcoxon tests, and linear discriminant analysis effect size (LEfSe). No significant differences were observed in either α or β diversity metrics between PMA-treated and nontreated samples. However, PMA treatment significantly reduced the abundance of farm environment–associated Dickeya and Pectobacteriaceae taxa in cheese samples, thus improving the accuracy of determining the cheese microbial structure using next-generation sequencing technologies. Further longitudinal studies focusing on different sampling periods during ripening, as well as other cheese types, may shed light on the potential benefits of using PMA for improving the accuracy of cheese microbial community characterization by next-generation sequencing. Summary: This study evaluated whether propidium monoazide (PMA) treatment affects the characterization of microbial communities in 60-day-ripened raw goat cheese using 16S ribosomal RNA (rRNA) metabarcoding. The PMA selectively depletes DNA from nonviable cells, potentially providing a more accurate representation of living microorganisms. Paired samples (PMA-treated and nontreated controls) from the same cheese units were analyzed using Illumina MiSeq sequencing, with downstream analyses performed in QIIME 2 and R. Results showed no significant differences in alpha or beta diversity metrics between treatments, indicating that PMA did not alter the overall microbial community structure. However, PMA treatment significantly reduced the abundance of farm environment-associated taxa, specifically Dickeya and Pectobacteriaceae, suggesting these organisms were predominantly nonviable with residual DNA persisting in the cheese matrix. This demonstrates that PMA pretreatment improves the accuracy of cheese microbiome characterization by filtering dead cell DNA from environmental contaminants, providing clearer insights into viable microbial communities during cheese ripening.</summary>
    <dc:date>2026-01-01T00:00:00Z</dc:date>
  </entry>
  <entry>
    <title>Avaliação da contagem de células somáticas e composição do leite de cabra cru em propriedades da Paraíba e de Pernambuco.</title>
    <link rel="alternate" href="https://www.alice.cnptia.embrapa.br/alice/handle/doc/1188167" />
    <author>
      <name>O. FILHO, F. E. M.</name>
    </author>
    <author>
      <name>SILVA, M. F.</name>
    </author>
    <author>
      <name>MORAIS, C. G. A.</name>
    </author>
    <author>
      <name>SOUZA, V. de</name>
    </author>
    <id>https://www.alice.cnptia.embrapa.br/alice/handle/doc/1188167</id>
    <updated>2026-07-12T16:47:05Z</updated>
    <published>2026-01-01T00:00:00Z</published>
    <summary type="text">Título: Avaliação da contagem de células somáticas e composição do leite de cabra cru em propriedades da Paraíba e de Pernambuco.
Autoria: O. FILHO, F. E. M.; SILVA, M. F.; MORAIS, C. G. A.; SOUZA, V. de
Conteúdo: A caprinocultura leiteira representa uma atividade socioeconômica fundamental para a agricultura familiar no Nordeste brasileiro. Contudo, a qualidade do leite produzido é frequentemente comprometida por falhas de manejo nutricional e sanitário. A mastite, processo inflamatório da glândula mamária, destaca-se como uma das principais enfermidades observadas nos rebanhos, provocando prejuízos econômicos e alterações físico-químicas no produto final. O objetivo deste estudo foi avaliar os parâmetros composicionais e a contagem de células somáticas (CCS) de amostras de leite de cabra cru obtidas em propriedades dos municípios da Paraíba e de Pernambuco.</summary>
    <dc:date>2026-01-01T00:00:00Z</dc:date>
  </entry>
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