DSpace Communidade: Embrapa Caprinos (CNPC)Embrapa Caprinos (CNPC)https://www.alice.cnptia.embrapa.br/alice/handle/item/112026-07-12T11:54:30Z2026-07-12T11:54:30ZEvaluation of propidium monoazide for 16S ribosomal RNA metabarcoding assessment of microbial communities in 60-day ripened raw goat milk cheese.FEITOZA, S. N. P.LIMA, L. A. deSARAIVA, C. A. S.DIAS, W. da S.MEDEIROS, A. B. A. deFERNANDES, A. C. de C.HEINEMANN, M. B.SOUZA, F. N. deFELISBERTO, N. R. de O.LEMOS, M. L. P.EGITO, A. S. doOLIVEIRA, C. J. B. dehttps://www.alice.cnptia.embrapa.br/alice/handle/doc/11882042026-07-10T13:54:44Z2026-01-01T00:00:00ZTítulo: Evaluation of propidium monoazide for 16S ribosomal RNA metabarcoding assessment of microbial communities in 60-day ripened raw goat milk cheese.
Autoria: FEITOZA, S. N. P.; LIMA, L. A. de; SARAIVA, C. A. S.; DIAS, W. da S.; MEDEIROS, A. B. A. de; FERNANDES, A. C. de C.; HEINEMANN, M. B.; SOUZA, F. N. de; FELISBERTO, N. R. de O.; LEMOS, M. L. P.; EGITO, A. S. do; OLIVEIRA, C. J. B. de
Conteúdo: Abstract: Cheese ripening is a complex microbial process marked by significant shifts in microbial composition. Considering that propidium monoazide (PMA) depletes DNA from nonviable cells, we hypothesized that PMA treatment of cheese samples could affect the microbiota characterization of 60-d-ripened raw goat curd cheese by 16S rRNA metabarcoding sequencing. After ripening, PMA-treated and nontreated (control) samples from the same cheese units were processed for DNA extraction, library preparation, and 16S rRNA metabarcoding sequencing on an Illumina MiSeq platform. Downstream bioinformatic analyses for microbial diversity assessment were performed using QIIME 2 and the phyloseq package in R. Statistical analyses included permutational multivariate analysis of variance (PERMANOVA), Wilcoxon tests, and linear discriminant analysis effect size (LEfSe). No significant differences were observed in either α or β diversity metrics between PMA-treated and nontreated samples. However, PMA treatment significantly reduced the abundance of farm environment–associated Dickeya and Pectobacteriaceae taxa in cheese samples, thus improving the accuracy of determining the cheese microbial structure using next-generation sequencing technologies. Further longitudinal studies focusing on different sampling periods during ripening, as well as other cheese types, may shed light on the potential benefits of using PMA for improving the accuracy of cheese microbial community characterization by next-generation sequencing. Summary: This study evaluated whether propidium monoazide (PMA) treatment affects the characterization of microbial communities in 60-day-ripened raw goat cheese using 16S ribosomal RNA (rRNA) metabarcoding. The PMA selectively depletes DNA from nonviable cells, potentially providing a more accurate representation of living microorganisms. Paired samples (PMA-treated and nontreated controls) from the same cheese units were analyzed using Illumina MiSeq sequencing, with downstream analyses performed in QIIME 2 and R. Results showed no significant differences in alpha or beta diversity metrics between treatments, indicating that PMA did not alter the overall microbial community structure. However, PMA treatment significantly reduced the abundance of farm environment-associated taxa, specifically Dickeya and Pectobacteriaceae, suggesting these organisms were predominantly nonviable with residual DNA persisting in the cheese matrix. This demonstrates that PMA pretreatment improves the accuracy of cheese microbiome characterization by filtering dead cell DNA from environmental contaminants, providing clearer insights into viable microbial communities during cheese ripening.2026-01-01T00:00:00ZAvaliação da contagem de células somáticas e composição do leite de cabra cru em propriedades da Paraíba e de Pernambuco.O. FILHO, F. E. M.SILVA, M. F.MORAIS, C. G. A.SOUZA, V. dehttps://www.alice.cnptia.embrapa.br/alice/handle/doc/11881672026-07-09T15:48:46Z2026-01-01T00:00:00ZTítulo: Avaliação da contagem de células somáticas e composição do leite de cabra cru em propriedades da Paraíba e de Pernambuco.
Autoria: O. FILHO, F. E. M.; SILVA, M. F.; MORAIS, C. G. A.; SOUZA, V. de
Conteúdo: A caprinocultura leiteira representa uma atividade socioeconômica fundamental para a agricultura familiar no Nordeste brasileiro. Contudo, a qualidade do leite produzido é frequentemente comprometida por falhas de manejo nutricional e sanitário. A mastite, processo inflamatório da glândula mamária, destaca-se como uma das principais enfermidades observadas nos rebanhos, provocando prejuízos econômicos e alterações físico-químicas no produto final. O objetivo deste estudo foi avaliar os parâmetros composicionais e a contagem de células somáticas (CCS) de amostras de leite de cabra cru obtidas em propriedades dos municípios da Paraíba e de Pernambuco.2026-01-01T00:00:00ZEffect of different cutting intensities on morphological characteristics and production of fresh and dry matter of irrigated Nopalea forage cactus.LIMA, G. F. da C.RÊGO, M. M. T.SILVA, J. G. M.AGUIAR, E. M.DANTAS, F. D. G.GUEDES, F. X.SILVA, G. J. A. M.LOBO, R. N. B.https://www.alice.cnptia.embrapa.br/alice/handle/doc/11877612026-06-28T12:36:37Z2013-01-01T00:00:00ZTítulo: Effect of different cutting intensities on morphological characteristics and production of fresh and dry matter of irrigated Nopalea forage cactus.
Autoria: LIMA, G. F. da C.; RÊGO, M. M. T.; SILVA, J. G. M.; AGUIAR, E. M.; DANTAS, F. D. G.; GUEDES, F. X.; SILVA, G. J. A. M.; LOBO, R. N. B.
Conteúdo: The spineless cactus (Nopalea cochenillifera Salm Dick) is adapted to the Brazilian semi-arid region, because of its high efficiency in water use and forage production. On the other hand, in some regions of Rio Grande do Norte State, because of drought and high temperatures, mainly nocturnal, usually plants wilt. The use of drip irrigation with a minimum use of water may be an option for cactus pear cultivation in these regions. The research aimed to evaluate the effect of different cutting intensities on the morphological characteristics and production of fresh and dry matter of irrigated Nopalea forage cactus after 12 months of regrowth.2013-01-01T00:00:00ZGenetic parameters estimates of growth traits of Santa Inês sheep using the multiple-trait model.ALVES, A. A. C.PORCIÚNCULA, J. A. daMARTINS JUNIOR, C. T.SOUSA, D. R. deSILVA, M. S. daLOBO, R. N. B.https://www.alice.cnptia.embrapa.br/alice/handle/doc/11877682026-06-28T12:36:42Z2013-01-01T00:00:00ZTítulo: Genetic parameters estimates of growth traits of Santa Inês sheep using the multiple-trait model.
Autoria: ALVES, A. A. C.; PORCIÚNCULA, J. A. da; MARTINS JUNIOR, C. T.; SOUSA, D. R. de; SILVA, M. S. da; LOBO, R. N. B.
Conteúdo: Abstract: The aim of this study was to estimate genetic parameters for Santa Inês sheep’s weight during their birth (BW), weaning (WW), slaughter (SW) and at the age of one (W1). The data used in this study come from a flock which belongs to the company Gaasa Alimentos LTDA, located in Inhumás (a town in the Brazilian state of Goiás), and they are part of the Programme of Genetic Improvement of Goats and Sheep (GENECOC) managed by the company Embrapa Goats and Sheep. The parentage matrix used consisted of 13,440 animals.2013-01-01T00:00:00Z