DSpace Coleção: Nota Técnica/Nota científica (CNPH)Nota Técnica/Nota científica (CNPH)https://www.alice.cnptia.embrapa.br/alice/handle/item/1812026-04-23T06:01:22Z2026-04-23T06:01:22ZFirst report of diaporthe arecae causing dieback in mangifera indica in Brazil.AMARAL, A. G. G.DUARTE, I. G.REIS, A.CAPUCHO, A. S.MILLER, S.CASTLEBURY, L. A.CÂMARA, M. P. S.https://www.alice.cnptia.embrapa.br/alice/handle/doc/11845802026-03-01T04:42:38Z2025-01-01T00:00:00ZTítulo: First report of diaporthe arecae causing dieback in mangifera indica in Brazil.
Autoria: AMARAL, A. G. G.; DUARTE, I. G.; REIS, A.; CAPUCHO, A. S.; MILLER, S.; CASTLEBURY, L. A.; CÂMARA, M. P. S.
Conteúdo: In 2024, dieback was detected on mango trees in Pernambuco, Brazil, with 40% disease incidence in all areas visited. Three isolates (MP22, MP27 and MP29) were obtained. Based on morphology, cultural and molecular analysis, the causal agent was identified as Diaporthe arecae. Koch's postulates were fulfilled by pathogenicity tests carried out on mango tree branches (cv. Tommy Atkins). To our knowledge, this is the first report of D. arecae causing dieback in mango (cv. Tommy Atkins) in Brazil.2025-01-01T00:00:00ZPCR with specific primers for detection of Rhizoctonia solani anastomosis groups reveals lack of specificity.AMANCIO, L. C. S.MACHADO, A. R.SOUZA-MOTTA, C. M. deTOVAR-PEDRAZA, J. M.REIS, A.CORREIA, K. C.MICHEREFF, S. J.https://www.alice.cnptia.embrapa.br/alice/handle/doc/11843962026-02-15T03:00:46Z2025-01-01T00:00:00ZTítulo: PCR with specific primers for detection of Rhizoctonia solani anastomosis groups reveals lack of specificity.
Autoria: AMANCIO, L. C. S.; MACHADO, A. R.; SOUZA-MOTTA, C. M. de; TOVAR-PEDRAZA, J. M.; REIS, A.; CORREIA, K. C.; MICHEREFF, S. J.
Conteúdo: Several specific primers based on the internal transcribed spacer (ITS) region of rDNA have been used to discriminate anastomosis groups (AGs) and subgroups in Rhizoctonia solani, but the efficacy of these primers was not evaluated considering several known AGs. This study aimed to evaluate the efficacy of seven PCR-specific primers for the detection of four AGs and subgroups of R. solani (AG-1 IA, AG-1 IB, AG-2-1, AG-3 PT, AG-3TB, AG-4 HGI, and AG-4 HGII). Thirteen isolates of R. solani and seven isolates of binucleate Rhizoctonia belonging to different AGs and subgroups were used in the detection assays and were amplified and sequenced using ITS1 and ITS4 universal primers to confirm the previous identification of AGs and the viability of the DNA samples. In addition, three isolates of unrelated fungal species (Fusarium oxysporum, Macrophomina phaseolina, and Sclerotium rolfsii) were tested simultaneously with each primer set above as a negative control. All primers tested nonspecifically amplified other AGs, and most of the primers produced bands for unrelated fungal species. Therefore, the exclusive use of these primers under the PCR conditions should be avoided due to the lack of accuracy in the results.2025-01-01T00:00:00ZFirst report of infestation by Planococcus citri (Risso, 1813) (Hemiptera: Pseudococcidae) on Cyperus esculentus (Poales: Cyperaceae).GODOI JUNIOR, M. A.SOUSA, R. N.MENDES, K. F.PINTO, C. M. F.RIBEIRO W. S.https://www.alice.cnptia.embrapa.br/alice/handle/doc/11690522025-03-16T06:43:11Z2024-01-01T00:00:00ZTítulo: First report of infestation by Planococcus citri (Risso, 1813) (Hemiptera: Pseudococcidae) on Cyperus esculentus (Poales: Cyperaceae).
Autoria: GODOI JUNIOR, M. A.; SOUSA, R. N.; MENDES, K. F.; PINTO, C. M. F.; RIBEIRO W. S.
Conteúdo: Therefore, our aim was to report, for the first time, the occurrence of P. citri attacking tubers of C. esculentus. Observations were conducted in an experimental area of the Universidade Federal de Viçosa (UFV), in the Zona da Mata region of Minas Gerais, Brazil, over two cycles in 2023. In this area, experiments were being conducted to evaluate the development of technologies for the cultivation of C. esculentus in the Zona da Mata region of Minas Gerais.2024-01-01T00:00:00ZOn the assessment of the sources of inoculum of bacterial wilt in Brazil.MIZUBUTI, E. S. G.YAMADA, J. K.SANTIAGO, T. R.LOPES, C. A.https://www.alice.cnptia.embrapa.br/alice/handle/doc/11519412025-03-16T08:17:21Z2022-01-01T00:00:00ZTítulo: On the assessment of the sources of inoculum of bacterial wilt in Brazil.
Autoria: MIZUBUTI, E. S. G.; YAMADA, J. K.; SANTIAGO, T. R.; LOPES, C. A.
Conteúdo: Dispersal of Ralstonia spp. cells by water and contaminated plant material and the importance of weeds as inoculum sources have been poorly investigated. Water of rivers, soil from fields of diverse crops and areas of natural vegetation both from the Amazônia, Cerrado and Mata Atlântica biomes, besides soil of the rhizosphere of weeds present in tomato fields with records of bacterial wilt were sampled and analyzed to detect Ralstonia spp. Seeds of tomato plants artificially and naturally infected with Ralstonia spp. were also processed. All samples were enriched a priori in selective medium South Africa (SMSA) and colonies were isolated in plates containing solid SMSA. Detection of Ralstonia spp. was confirmed by polymerase chain reaction with specific primers. The Co ? operational PCR (CO-PCR) was also used to detect Ralstonia spp. Colonies were obtained from soil samples and from a commercial substrate sample. Five soil samples from eggplant fields, one from coffee field, one substrate from potato seed tuber production, two soil samples from the rhizosphere of Amaranthus spp., one from Bidens pilosa and one from Solanum americanum tested positive for Ralstonia spp. Besides these soil samples, five water samples of rivers were positive for CO-PCR detection: two samples from Amazônia, one from Cerrado and two samples from irrigation water collected from tomato fields located in the Mata Atlântica biome. Ralstonia spp. were not detected in tomato seeds. These results revealed potential inoculum sources, especially weeds, in areas with historical records of bacterial wilt. Additionally, rivers may act as dispersal agents of inoculum of Ralstonia spp.2022-01-01T00:00:00Z