Artigo em periódico indexado (CNPAE) https://www.alice.cnptia.embrapa.br/alice/handle/item/394 2026-06-14T18:27:52Z 2026-06-14T18:27:52Z HAMANN, P. R. V. MARTINS, H. S. COSTA, P. H. C. da BRITO, D. T. de A. SILVA, V. R. C. da TAVARES, Y. F. QUIRINO, B. F. NORONHA, E. F. https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187414 2026-06-14T17:55:00Z 2026-01-01T00:00:00Z

Título: Enhancing wheat bran deconstruction with enzyme cocktails from Penicillium spp. and Trichoderma harzianum: the impact of β-glucosidase supplementation. Autoria: HAMANN, P. R. V.; MARTINS, H. S.; COSTA, P. H. C. da; BRITO, D. T. de A.; SILVA, V. R. C. da; TAVARES, Y. F.; QUIRINO, B. F.; NORONHA, E. F. Conteúdo: Abstract: Forests and natural environments are valuable sources of organisms capable of degrading plant cell wall carbohydrates. In the present study, two isolates, Penicillium polonicum and Penicillium chrysogenum, obtained from Brazilian Cerrado soil, along with a previously characterized isolate of Trichoderma harzianum, were evaluated for their ability to produce carbohydrate-active enzymes when grown in the presence of wheat bran. P. chrysogenum exhibited higher production of endoglucanases, xylanases, and pectinases. Biochemical characterization showed that most enzymes were active at pH 5 and within a temperature range of 30–50 °C, with the exception of P. chrysogenum endoglucanases, which displayed optimal activity at 60 °C. Hydrolysis of cellulose and carboxymethyl cellulose by the three enzyme sources demonstrated that supplementation with recombinant β-glucosidase from Clostridium thermocellum (BglA) significantly enhanced reducing sugar release; specifically, when applied to P. chrysogenum, the reducing sugar yield from cellulose hydrolysis increased by 2.53-fold. In the hydrolysis of wheat bran, enzymes from P. chrysogenum and T. harzianum supplemented with BglA resulted in a 1.6- and 3.93-fold increase in D-glucose yield, respectively. The results presented in this study contribute to the development of more robust enzyme cocktails for wheat bran valorization.

2026-01-01T00:00:00Z SANTOS, G. S. CAMPOS, F. C. de https://www.alice.cnptia.embrapa.br/alice/handle/doc/885255 2017-08-15T21:23:00Z 2009-01-01T00:00:00Z

Título: Gerenciamento de projetos em TI: um estudo de caso do plano de transição de serviços. Autoria: SANTOS, G. S.; CAMPOS, F. C. de

2009-01-01T00:00:00Z SANTOS, G. S. CAMPOS, F. C. de https://www.alice.cnptia.embrapa.br/alice/handle/doc/885259 2017-08-15T21:24:17Z 2009-01-01T00:00:00Z

Título: Gestão do conhecimento em serviços de TI: um estudo do uso do modelo ITIL-SKMS em monitoramento de infra-estrutura de TI. Autoria: SANTOS, G. S.; CAMPOS, F. C. de

2009-01-01T00:00:00Z SANTOS, D. T. dos PINHO, M. https://www.alice.cnptia.embrapa.br/alice/handle/doc/884867 2017-08-15T21:49:47Z 2010-01-01T00:00:00Z

Título: Análise do crescimento das empresas de base tecnológica no Brasil. Autoria: SANTOS, D. T. dos; PINHO, M.

2010-01-01T00:00:00Z