Embrapa Agroenergia (CNPAE) https://www.alice.cnptia.embrapa.br/alice/handle/item/41 2026-06-24T00:58:01Z 2026-06-24T00:58:01Z LAMAS, G. C. CARDOSO, A. N. SABAINI, P. S. LUZ, S. M. BORGES, M. dos R. S. COSTA, T. da S. GONZALES, T. da S. MATSUURA, M. I. da S. F. LAVIOLA, B. G. RODRIGUES, T. O. ROUSSET, P. SILVEIRA, E. A. https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187682 2026-06-21T10:47:30Z 2026-01-01T00:00:00Z

Título: Energy–environmental nexus in green hydrogen-assisted conversion of second-crop canola to sustainable aviation fuel: toward low-carbon bioenergy systems. Autoria: LAMAS, G. C.; CARDOSO, A. N.; SABAINI, P. S.; LUZ, S. M.; BORGES, M. dos R. S.; COSTA, T. da S.; GONZALES, T. da S.; MATSUURA, M. I. da S. F.; LAVIOLA, B. G.; RODRIGUES, T. O.; ROUSSET, P.; SILVEIRA, E. A. Conteúdo: Abstract: Aviation fuel remains the main cost and environmental burden in air transport. This study presents a well-to-wake life cycle assessment (LCA) of canola-based Sustainable Aviation Fuel (SAF) under tropical conditions, based on primary data from Brazilian producers. The analysis encompasses agricultural, pre-processing, and conversion stages via the hydroprocessed esters and fatty acids (HEFA) pathway, revealing the potential of second-crop canola for low-carbon aviation. The study integrates process modeling, renewable hydrogen, and land-use efficiency to capture drivers across stages. The ReCiPe method was applied to 1 MJ of biokerosene as the functional unit. Agriculture dominates GHG emissions (34.2 g CO2 eq. MJ−1), driven primarily by fertilizer production and soil N2O emissions, while the HEFA phase contributes 12.8 g CO2 eq. MJ−1. Substituting fossil hydrogen with photovoltaic- and wind-based hydrogen for in HEFA upgrading reduces emissions by 92 to 96.6%, resulting in up to 19.6% lower total life-cycle emissions. Compared to Jet-A1, SAF decreases fossil depletion by 59% and achieves climate benefits; however, it entails higher burdens in selected non-climate impact categories. Freshwater and marine eutrophication reach approximately 0.01 g P eq. MJ−1 and 0.7 g N eq. MJ−1, respectively, while human toxicity is above 1 g 1,4-DB eq. MJ−1, with the agricultural stage accounting for over 90% of these impacts, particularly fertilizer production and use. Land occupation (0.074 m2 yr MJ−1) is optimized through canola soybean rotation, mitigating deforestation risks. The findings demonstrate canola's strategic role in Brazil's decarbonization policies, highlighting the need for improved fertilizer management and renewable hydrogen integration to advance SAF.

2026-01-01T00:00:00Z HAMANN, P. R. V. MARTINS, H. S. COSTA, P. H. C. da BRITO, D. T. de A. SILVA, V. R. C. da TAVARES, Y. F. QUIRINO, B. F. NORONHA, E. F. https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187414 2026-06-14T17:55:00Z 2026-01-01T00:00:00Z

Título: Enhancing wheat bran deconstruction with enzyme cocktails from Penicillium spp. and Trichoderma harzianum: the impact of β-glucosidase supplementation. Autoria: HAMANN, P. R. V.; MARTINS, H. S.; COSTA, P. H. C. da; BRITO, D. T. de A.; SILVA, V. R. C. da; TAVARES, Y. F.; QUIRINO, B. F.; NORONHA, E. F. Conteúdo: Abstract: Forests and natural environments are valuable sources of organisms capable of degrading plant cell wall carbohydrates. In the present study, two isolates, Penicillium polonicum and Penicillium chrysogenum, obtained from Brazilian Cerrado soil, along with a previously characterized isolate of Trichoderma harzianum, were evaluated for their ability to produce carbohydrate-active enzymes when grown in the presence of wheat bran. P. chrysogenum exhibited higher production of endoglucanases, xylanases, and pectinases. Biochemical characterization showed that most enzymes were active at pH 5 and within a temperature range of 30–50 °C, with the exception of P. chrysogenum endoglucanases, which displayed optimal activity at 60 °C. Hydrolysis of cellulose and carboxymethyl cellulose by the three enzyme sources demonstrated that supplementation with recombinant β-glucosidase from Clostridium thermocellum (BglA) significantly enhanced reducing sugar release; specifically, when applied to P. chrysogenum, the reducing sugar yield from cellulose hydrolysis increased by 2.53-fold. In the hydrolysis of wheat bran, enzymes from P. chrysogenum and T. harzianum supplemented with BglA resulted in a 1.6- and 3.93-fold increase in D-glucose yield, respectively. The results presented in this study contribute to the development of more robust enzyme cocktails for wheat bran valorization.

2026-01-01T00:00:00Z BERGMANN, J. C. LACERDA, V. A. M. ALENCAR, K. L. C. FAVARO, L. C. de L. RODRIGUES, D. de S. MARINS, L. F. QUIRINO, B. F. https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187279 2026-06-06T12:56:35Z 2026-01-01T00:00:00Z

Título: A new GH3 B-Glucosidase from Chryseobacterium sp. with applications in cellulosic ethanol production and agri-biotechnological processes. Autoria: BERGMANN, J. C.; LACERDA, V. A. M.; ALENCAR, K. L. C.; FAVARO, L. C. de L.; RODRIGUES, D. de S.; MARINS, L. F.; QUIRINO, B. F. Conteúdo: Abstract: β-Glucosidases catalyze the hydrolysis of β-glycosidic bonds and play key roles in biomass conversion and glycoside processing. We report the identification and characterization of Cr_B1, a GH3 β-glucosidase from Chryseobacterium sp. containing a predicted signal peptide. Cr_B1 hydrolyzed pNPG, cellobiose, salicin, and daidzin, showing optimal activity at pH 5.0−5.5 and 45−55 °C. The enzyme retained over 90% activity after 190 days at 4°C and 25 °C and above 80% activity after 24 h at 50 °C, indicating remarkable long-term and thermal stability. Cr_B1 exhibited high glucose tolerance (IC50: 1.5−1.8 M) and substrate-dependent kinetics. In synergy with Celluclast, it increased glucose release from CMC by 69%, demonstrating its potential to enhance enzymatic saccharification. These properties highlight Cr_B1 as a promising biocatalyst for improving saccharification, enhancing isoflavone bioavailability, and reducing bitterness in food and feed applications.

2026-01-01T00:00:00Z SOUZA, A. P. R. de PASQUINI, D. VAZ JUNIOR, S. https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187275 2026-06-06T12:56:16Z 2025-01-01T00:00:00Z

Título: Obtenção, caracterização e avaliação de membranas poliméricas à base de lignina e amido termoplástico para separação de CO2. Autoria: SOUZA, A. P. R. de; PASQUINI, D.; VAZ JUNIOR, S. Conteúdo: Resumo: A tecnologia de separação de gases através de membranas se mostra promissora diante da sua processabilidade e aplicação. Esse trabalho tem como objetivo obter e caracterizar membranas poliméricas renováveis (MPR) para separação de dióxido de carbono (CO2). A metodologia consiste em duas etapas: 1) oxipropilação de ligninas e 2) obtenção de MPR a partir da adição de amido termoplástico (ATP). FTIR, TGA e MEV foram realizadas nas MPR. Dióxido de titânio (TiO2) foi adicionado ao material visando a otimização dos parâmetros de permeação. As MPR sem TiO2 não se mostraram favoráveis à permeação de nitrogênio (N2) e CO2, enquanto as MPR com TiO2 apresentaram resultados promissores para a permeação de CO2. Diante disso, a utilização de TiO2 se faz essencial na composição das MPR.

2025-01-01T00:00:00Z