Artigo em periódico indexado (CPAA) https://www.alice.cnptia.embrapa.br/alice/handle/item/97 2026-08-12T06:47:00Z 2026-08-12T06:47:00Z ARAÚJO, R. L. SANTOS, P. R. dos BARBOSA, K. R. de S. CHAVES, F. C. M. CHAGAS, E. C. https://www.alice.cnptia.embrapa.br/alice/handle/doc/1188873 2026-08-03T21:34:15Z 2026-01-01T00:00:00Z

Título: Antimicrobial activity of nine essential oils against Aeromonas spp. isolated from tambaqui (Colossoma macropomum Cuvier, 1816). Autoria: ARAÚJO, R. L.; SANTOS, P. R. dos; BARBOSA, K. R. de S.; CHAVES, F. C. M.; CHAGAS, E. C. Conteúdo: The use of natural products with antimicrobial activity is an effective and sustainable alternative for the prevention, treatment, and/or control of diseases in aquaculture. The objective of this study was to determine the chemical composition and antimicrobial activity of nine essential oils (EOs) against strains of Aeromonas jandaei and Aeromonas hydrophila isolated from tambaqui (Colossoma macropomum). Essential oils were obtained by hydrodistillation and their composition by gass chromatography coupled with mass spectrometry. Antimicrobial activity of the nine essential oils was evaluated based on the Minimum Inhibitory Concentration (MIC), using the microdilution method, and Minimum Bactericidal Concentration (MBC). The major components identified in EO of Croton sacaquinha, Cymbopogon nardus, Curcuma longa, Lippia origanoides, Mentha arvensis, Ocimum basilicum, Ocimum micranthum, Piper hispidum e Piper marginatum were β-selinene (14.5%), geraniol (53.3%), ar-turmerone (30.2%), carvacrol (43.3%), menthol (88.5%), methyl chavicol (33.9%), piperitone oxide (36.2%), γ-terpinene (37.1%) and δ-3-carene (13.1%), respectively. The EOs of L. origanoides (9.2 mg·mL−1), C. nardus (10 mg·mL−1), M. arvensis, and P. marginatum (15 mg·mL−1) exhibited the most pronounced antimicrobial activity, whereas all essential oils tested demonstrated bactericidal activity against Aeromonas strains. Resumo: A utilização de produtos naturais com atividade antimicrobiana configura-se como uma alternativa eficaz e sustentável para a prevenção, tratamento e/ou controle de doenças na aquicultura. O presente estudo teve como objetivo determinar a composição química e atividade antimicrobiana de nove óleos essenciais (OEs) frente a cepas de Aeromonas jandaei e Aeromonas hydrophila isoladas de tambaqui (Colossoma macropomum). Os óleos essenciais foram obtidos por hidrodestilação e analisados por cromatografia gasosa acoplada a espectrometria de massa. A atividade antimicrobiana de nove OEs foi avaliada com base na determinação da Concentração Inibitória Mínima (CIM), pelo método de microdiluição em caldo, e da Concentração Bactericida Mínima (CBM). Os componentes majoritários identificados nos OEs de Croton sacaquinha, Cymbopogon nardus, Curcuma longa, Lippia origanoides, Mentha arvensis, Ocimum basilicum, Ocimum micranthum, Piper hispidum e Piper marginatum foram β-selineno (14,5%), geraniol (53,3%), ar-turmerona (30,2%), carvacrol (43,3%), mentol (88,5%) metil chavicol (33,9%), óxido de piperitona (36,2%), γ-terpineno (37,1%), e δ-3-careno (13,1%), respectivamente. Os OEs de L. origanoides (9,2 mg.ml-1), C. nardus (10 mg.ml-1), M. arvensis e P. marginatum (15 mg.ml-1) apresentaram atividade antimicrobiana mais expressiva; enquanto todos os OEs testados apresentaram atividade bactericida frente às cepas de Aeromonas.

2026-01-01T00:00:00Z MELO NETO, P. R. de OLIVEIRA, M. de F. PEREIRA, O. de S. SCARIOT, A. O. HENDERSON, A. J. PACHECO, M. A. W. CAMARGO, J. L. C. https://www.alice.cnptia.embrapa.br/alice/handle/doc/1188743 2026-08-01T13:28:18Z 2026-01-01T00:00:00Z

Título: Palm assembly dynamics and its relationship with topography in terra firme forest in the Central Amazon. Autoria: MELO NETO, P. R. de; OLIVEIRA, M. de F.; PEREIRA, O. de S.; SCARIOT, A. O.; HENDERSON, A. J.; PACHECO, M. A. W.; CAMARGO, J. L. C. Conteúdo: In recent years, studies have reported changes in the structure of different taxonomic groups in tropical forests without any apparent environmental disturbances. Changes in forest phytosociology, climate change, or even stochastic variation may be responsible for the observed results. Factors such as topography may mitigate or amplify these effects. We analyzed the long-term dynamics of an assembly of palm species in a 10-ha permanent plot within a continuous, mature forest in the Central Amazon. A previous census was conducted in 1990. As previously, all newly found adult palms were marked, identified, and classified by forest strata. Thus, we evaluated assembly dynamics overall and by strata, considering abundance, composition, and beta diversity. All parameters were contrasted with topography. We recorded 33 palm species, three more species than in 1990, and an impressive increase in abundance. Even with 1/3 of mortality, the net increase of the assembly was almost 50%, revealing a dramatic change in density from around 650 to almost 1000 adult palms per hectare. Mortality among the sub-canopy palm trees was more pronounced than for other strata. Meanwhile, the forest's understory presented the highest diversity. Species gain over time drove changes in the assemblage composition. Regarding topography, elevation influenced species distribution, while slope affected understory and assemblage dynamics. Thus, changes in the palm assemblage showed that even in forests considered preserved, certain groups are undergoing structural changes, which may be related to internal assemblage factors or external drivers, such as climate change and stochastic effects.

2026-01-01T00:00:00Z BATISTA, S. R. MAJOLO, C. PERIN, R. NOGUEIRA, M. A. HUNGRIA, M. OLIVEIRA-PAIVA, C. A. SÁ, E. L. S. de MÜLLER, C. LOPES, M. T. G. MUNIZ, A. W. https://www.alice.cnptia.embrapa.br/alice/handle/doc/1188201 2026-07-12T16:47:35Z 2026-01-01T00:00:00Z

Título: Plant growth- promoting bacteria inoculation and co-inoculation effects on Palisade grass (Urochloa brizantha cv. Marandu) in ferralsol in Amazonas State, Brazil. Autoria: BATISTA, S. R.; MAJOLO, C.; PERIN, R.; NOGUEIRA, M. A.; HUNGRIA, M.; OLIVEIRA-PAIVA, C. A.; SÁ, E. L. S. de; MÜLLER, C.; LOPES, M. T. G.; MUNIZ, A. W. Conteúdo: Deforestation is primarily driven by three main factors the conversion of the Amazon rainforest into pastureland, agriculture, and mining, as well as land invasion. These pastures are mostly degraded, and it is necessary to restore their productive capacity. Microbial inoculants can be used to increase nutrient use efficiency. The objective of this study was to evaluate the effects of inoculation and co-inoculation of the bacteria Rhizobium anhuiense (RZ), Azospirillum brasilense (AZ) and Pseudomonas fluorescens (PF) on the growth of the grass Urochloa brizantha cv. Marandu. The field experiment with inoculants in U. brizantha was carried out in a randomized block design with four replications. The treatments consisted of different doses of NPK and types of inoculation. The variables used in the evaluation were plant height, number of tillers, productivity, and foliar NPK content. The results showed an interaction between fertilizer doses and inoculation treatments for variables such as number of tillers and productivity. Co-inoculation positively influenced productivity and plant nutrient content in some combinations and fertilizer doses. These findings suggest that inoculation and co-inoculation with different bacteria used different fertilizer doses, increasing the number of tillers, dry mass (P and K), and yield of Palisade grass as observed in co-inoculation with AZ + RZ.

2026-01-01T00:00:00Z PEREIRA, T. B. LOPES, E. V. CHAGAS, E. C. PEREIRA, D. da S. POHLIT, A. M. https://www.alice.cnptia.embrapa.br/alice/handle/doc/1188102 2026-07-12T16:46:50Z 2026-01-01T00:00:00Z

Título: Efeito anti-helmíntico in vitro da espécie Andira vermifuga contra Neoechinorhynchus buttnerae. Autoria: PEREIRA, T. B.; LOPES, E. V.; CHAGAS, E. C.; PEREIRA, D. da S.; POHLIT, A. M. Conteúdo: RESUMO: A infecção pelo acantocéfaloNeoechinorhynchus buttneraeconstitui um dos principais entraves à produção de tambaqui (Colossoma macropomum) na Amazônia. Este estudo avaliou a atividade anti-helmíntica in vitrode extratos e frações deAndira vermifugacontraN. buttnerae. As folhas foram secas, trituradas e submetidas à maceração sequencial com solventes de polaridade crescente (hexano, acetato de etila e metanol). O extrato metanólico e as frações hidroalcoólicas dos extratos hexânico e de acetato de etila foram testados in vitrocontra acantocéfalos de tambaquis naturalmente infectados. Todas as amostras promoveram 100% de mortalidade deN. buttneraeapós 24 h nas concentrações de 25 e 50 mg/L. A fração hidroalcoólica do extrato em acetato de etila foi a mais promissora, atingindo 100% de mortalidade em 6 h a 25 mg/L e apresentando elevada eficácia mesmo em baixas concentrações. Os resultados evidenciam o potencial de extratos de média polaridade de plantas amazônicas como fonte de compostos antiparasitários eficazes no controle de infecções que afetam a piscicultura do tambaqui. ABSTRACT: Infection by the acanthocephalan Neoechinorhynchus buttnerae is one of the main obstacles totambaqui (Colossoma macropomum) production in the Amazon. This study evaluated the in vitro anthelmintic activity of extracts and fractions of Andira vermifuga against N. buttnerae. The leaves were dried, crushed, and subjected to sequential maceration with solvents of increasing polarity (hexane, ethyl acetate, and methanol). The methanolic extract and the hydroalcoholic fractions of the hexane and ethyl acetate extracts were tested in vitro against acanthocephalans of naturally infected tambaqui. All samples promoted 100% mortality of N. buttnerae after 24 h at concentrations of 25 and 50 mg/L. The hydroalcoholic fraction of the ethyl acetate extract was the most promising, achieving 100% mortality in 6 hours at 25 mg/L and showing high efficacy even at low concentrations. The results highlight the potential of medium-polarity extracts from Amazonian plants as a source of effective antiparasitic compounds for controlling infections affecting tambaqui fish farming. RESUMEN: La infección por el acantocéfalo Neoechinorhynchus buttnerae es uno de los principales obstáculos para la producción de tambaquí (Colossoma macropomum) en la Amazonía. Este estudio evaluó la actividad antihelmíntica in vitro de extractos y fracciones de Andira vermifuga contra N. buttnerae. Las hojas se secaron, trituraron y se sometieron a maceración secuencial con solventes de polaridad creciente (hexano, acetato de etilo y metanol). El extracto metanólico y las fracciones hidroalcohólicas de los extractos de hexano y acetato de etilo se probaron in vitro contra acantocéfalos de tambaquí infectado naturalmente. Todas las muestras promovieron una mortalidad del 100% de N. buttnerae después de 24 h a concentraciones de 25 y 50 mg/L. La fracción hidroalcohólica del extracto de acetato de etilo fue la más prometedora, alcanzando una mortalidad del 100 % en 6 horas a 25 mg/L y mostrando una alta eficacia incluso a bajas concentraciones. Los resultados destacan el potencial de los extractos de polaridad media de plantas amazónicas como fuente de compuestos antiparasitarios eficaces para el control de infecciones que afectan a la piscicultura de tambaquí.

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