DSpace Communidade: Embrapa Gado de Leite (CNPGL)

Handmade biopsy for genotyping of in vitro-produced bovine embryos.

2026-01-01T00:00:00Z

Título: Handmade biopsy for genotyping of in vitro-produced bovine embryos. Autoria: CAMARGO, L. S. de A.; QUINTAO, C. C. R.; MARTINS, J. M. C. de L.; SILVA, M. V. G. B.; CAMARGO, A. J. dos R.; FREITAS, C. de; REIS, D. R. de L.; MACHADO, M. A.; OLIVEIRA, C. S. Conteúdo: Blastocyst biopsy allows genomic selection of pre-implantation bovine embryos, reducing generation interval and enhancing the genetic gain. We aimed to evaluate the suitability of a simple biopsy procedure, performed by hands, using a splitting microblade and a stereoscope, on in vitro-produced embryos. Crossbred Bos taurus x B. indicus blastocysts at Day 6 or 7 after in vitro fertilization (IVF) were biopsied and cultured in vitro for 48h. Biopsy reduced (p<0.01) embryo development as noted by the lower blastocoel formation/re-expansion rates in both Day 6 (33.3% vs. 71.4%) and Day 7 (66.6% vs. 89.8%) blastocysts, when compared to non-biopsied embryos. Biopsy on Day 7 decreased (p<0.01) cell number (112.8±6.3 vs. 149.9±5.6) and increased (p<0.01) apoptotic index (14.9±1.4 vs. 6.2±1.2). In a second experiment, the IVF blastocysts were biopsied in the farm, and cultured for 3h. Re-expanded blastocysts, corresponding to 78.3% biopsied embryos, were transferred to synchronized recipients. Pregnancy (57.4% vs. 62%), delivery (51.0% vs. 48%), gestation length (285.5±2.0 vs. 284.6±1.2days) and birthweight (30.5±1.8 vs. 29.9±1.2kg) were similar (p>0.05) between handmade biopsy and non-biopsied embryos. Whole genome amplification of biopsied samples resulted in 256.0±155.9ng/μL DNA. The mean call rate was 75.3%±4.3% and the imputed GEBV for 305-day milk yield was 839.21±61.6kg. In conclusion, handmade biopsy can be performed at small laboratories and at farm level, contributing to reduce costs for embryo genomic selection. Pregnancy, calf delivery and birthweight were not compromised, and samples are suitable for downstream applications such as genotyping.

Atividade residual do herbicida Imazaquin em cultivos sucessivos de girassol.

2008-01-01T00:00:00Z

Título: Atividade residual do herbicida Imazaquin em cultivos sucessivos de girassol. Autoria: BRIGHENTI, A. M.; FONTES, J. R. A.; ROCHA, W. S. D. da; MARTINS, C. E.; SOUZA SOBRINHO, F. de; STROPPA, G. M. Conteúdo: Este trabalho foi conduzido afim de avaliar o efeito residual no solo do herbicida imazaquin, aplicado na cultura da soja, sobre o girassol semeado em sucessão. O delineamento experimental foi blocos casualizados em parcelas subdivididas, com quatro repetições.

Polynomial model with asymmetric curvature induced by excess for mixture design: optimization of green anthocyanin extraction from Cenchrus purpureus using a standardless chromatographic response.

2026-01-01T00:00:00Z

Título: Polynomial model with asymmetric curvature induced by excess for mixture design: optimization of green anthocyanin extraction from Cenchrus purpureus using a standardless chromatographic response. Autoria: ADRIANO, L. H. C.; GERALDO, P. A.; NOGUEIRA, C. P.; MACHADO, J. C.; OLIVEIRA, M. A. L. de Conteúdo: Canonical Scheff´e mixture models are widely used for binary systems, but their inherent assumption of symmetric component behavior restricts their ability to describe processes in which mixture components fulfill distinct physicochemical roles. This limitation is especially relevant for solvent-based extraction systems involving water and ethanol. In this study, the extraction of anthocyanins from leaves of Cenchrus purpureus. using water:ethanol mixtures were investigated, and an alternative modeling framework, named Asymmetric Curvature Induced by Excess (ACIE), was proposed to overcome this conceptual constraint. Experimental and simulated approach were evaluated in this work. Extractions were carried out by static maceration at room temperature using only water and ethanol, in accordance with Green Chemistry principles. The extracts were analyzed by HPLC-DAD, and a semi-quantitative approach was adopted in which the response variable was defined as the sum of the chromatographic peak areas of the two most reproducible anthocyanin signals. Canonical Scheff´e linear and quadratic models were unable to adequately represent the system, exhibiting lack of fit and limited predictive capability. In contrast, ACIE, by incorporating asymmetric curvatures for modeling the excess of each component, achieved superior statistical performance and a more realistic representation of the response surface. The model predicted an optimal extraction region between 82 and 92% ethanol, consistent with the distinct roles of ethanol in promoting cell wall disruption and water in anthocyanin solubilization. These results show that ACIE effectively addresses a fundamental limitation of Scheff´e-based models relative to asymmetries in binary mixtures and provides a flexible, chemically interpretable framework that may be applicable to other systems characterized by asymmetric component functionality.

Biochemical characterization of a kunitz-type protease inhibitor from mimosa regnellii and its effects on melanoma cell viability and angiogenesis.

2026-01-01T00:00:00Z

Título: Biochemical characterization of a kunitz-type protease inhibitor from mimosa regnellii and its effects on melanoma cell viability and angiogenesis. Autoria: RABELO, L. M. A; CARDOSO, P. H. de O.; NOBRE, L. T. D. B.; SANTOS, P. I. M. dos; LUCENA, S. V.; SERQUIZ, R. P.; BEMQUERER, M. P.; ROCHA, H. A. de O.; NADER, H. B.; SANTOS, E. A. dos; OLIVEIRA, A. S. de; FRIHLING, B. E. F.; MIGLIOLO, L. Conteúdo: Cancer is a term used to represent more than 100 diseases, including malignant tumors of different localizations. Among these types, cutaneous melanoma is one of the most common types of skin cancer worldwide. Newer, non-invasive, and more effective forms of treatment are urgently needed. Recently, the cancer membrane has emerged as a novel target for new anticancer drugs. Serine protease inhibitors are researched across all kingdoms; however, the Plantae kingdom is a more sustainable producer of bioactive molecules, including serine protease inhibitors. In this study, a Kunitz trypsin inhibitor (JTI) purified by RP-HPLC from Mimosa regnellii seeds was sequenced, its inhibitory activity quantified, and its inhibition constant determined. It was then tested for its ability to induce cell death via the apoptotic pathway in B16-F10 mouse melanoma cells. To evaluate its pro-apoptotic capacity, the inhibitor’s effects on reactive oxygen speciesrelease, calcium release, cell morphological changes, and the inhibition of angiogenic and migratory activities of rabbit endothelial cells (RaEC) were also assessed in vitro. Tests for toxicity against normal cells were performed, confirming that JTI acts on melanoma cells with an IC50 of 0.65 µM. These results suggest that JTI has potential for use in melanoma treatment.