DSpace Coleção: Artigo em periódico indexado (CNPMF)

Traditional and transgenic strategies for controlling tomato-infecting begomoviruses.

2002-01-01T00:00:00Z

Título: Traditional and transgenic strategies for controlling tomato-infecting begomoviruses. Autoria: ASTUA, J. de F.; PURCIFULL, D. E.; POLSTON, J. E.; HIEBERT, E. Conteúdo: Viruses of to the family Geminiviridae are considered some of the most important pathogens in tropical and subtropical regions of the world. Members of one Geminiviridae genus, Begomovirus, have been causing severe losses, particularly in tomato (Lycopersicon esculentum) production in the Americas and the Caribbean. Several new begomoviruses have been reported in the region and, at least one, Tomato yellow leaf curl virus (TYLCV), has been brought in from the Old World via infected transplants. In addition, the recombination events that are playing an important role in Begomovirus diversity have increased the complexity of their control. This scenario has led to the search for control measures that go beyond traditional host genetic resistance, chemical controls and cultural practices. In this review, besides the recommended classical control measures, transgenic approaches will be discussed, as well as the mechanisms involved in their successful control of viruses.

High-throughput sequencing in phytopathology: genomics-driven diagnostics and host-pathogen interactions.

2026-01-01T00:00:00Z

Título: High-throughput sequencing in phytopathology: genomics-driven diagnostics and host-pathogen interactions. Autoria: PEREIRA, L. R.; BARRO, M. P.; HARAKAVA, R.; ASTUA, J. de F.; RAMOS-GONZÁLEZ, P. L. Conteúdo: ABSTRACT: Plant diseases severely constrain agricultural productivity, exacerbating food insecurity, economic instability, and environmental degradation. Global trade and climate change further intensify pathogen spread, emergence, and host shifts. While traditional diagnostics and targeted assays, such as polymerase chain reaction and enzyme-linked immunosorbent assay, improve specificity, they depend on prior knowledge and are limited in detecting novel or mixed infections. High-throughput sequencing (HTS) has emerged as a transformative, unbiased platform that allows comprehensive detection of known and unknown pathogens through metagenomics and transcriptomics. By generating large-scale genomic data, HTS supports pathogen discovery, epidemiological surveillance, quarantine systems, and genome-informed disease management. It underpins advanced strategies, including Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) proteins editing and RNA interference, and accelerates the breeding of resistance. Despite challenges – such as bioinformatics standardization, cost, and data interpretation – HTS, when integrated with classical diagnostics and biological validation, represents a foundational technology for sustainable, proactive plant health management and global phytosanitary resilience.

Sample size matters: optimizing fruit sampling for reliable quality assessment in sweet orange varieties

2026-01-01T00:00:00Z

Título: Sample size matters: optimizing fruit sampling for reliable quality assessment in sweet orange varieties Autoria: CALDAS, M. J. M.; SASAKI, F. F. C.; RODRIGUES, V. da S.; SANTANA, L. G. L.; LEDO, C. A. da S.; GESTEIRA, A. da S.; PASSOS, O. S.; SOARES FILHO, W. dos S. Conteúdo: ABSTRACT: This study aimed to determine the optimal sample size of fruits from sweet orange varieties for the evaluation of their physical and chemical quality attributes. Twenty ripe fruits from the following varieties, namely, Diva, Hamlin CNPMF-020, Pera CNPMF-D6, Uruburetama Blood, and Valência Tuxpan CNPMF were used. The variables assessed included fruit mass, longitudinal and transverse diameters, juice mass and yield, peel color and thickness, soluble solids content, titratable acidity, the soluble solids/titratable acidity ratio (SS/TA), and a technological index. The experimental design was completely randomized, with individual analysis of each fruit. Data were analyzed using the Modified Maximum Curvature Method with the aid of the R statistical software. The optimal minimum number of fruits varied according to variety and variable. The smallest required sample sizes were four fruits for Pera CNPMF-D6, six fruits for Diva and Uruburetama Blood, and seven fruits for Valência Tuxpan CNPMF and Hamlin CNPMF-020. Therefore, a standardized sample size of seven fruits is recommended for studies based on the physicochemical quality of sweet orange varieties, to ensure sufficient precision and statistical reliability under the conditions of this study.

Viabilidade de uso do híbrido Hawaii 7996 como porta-enxerto de cultivares comerciais de tomate.

2006-01-01T00:00:00Z

Título: Viabilidade de uso do híbrido Hawaii 7996 como porta-enxerto de cultivares comerciais de tomate. Autoria: CARDOSO, S. C.; SOARES, A. C. F.; BRITO, A. dos S.; CARVALHO, L. A.; LEDO, C. A. da S. Conteúdo: A técnica de enxertia é utilizada em algumas hortaliças, principalmente em áreas infestadas, para atribuir resistência a patógenos do solo, minimizando as perdas de produção de cultivares suscetíveis. Este trabalho teve como objetivo avaliar o desenvolvimento e a produtividade de plantas de tomateiro enxertadas em híbrido, tido como resistente a Ralstonia solanacearum, em comparação a pés-francos. O experimento foi desenvolvido em viveiro (produção de mudas e enxertia) e em céu aberto, no município de Cruz das Almas, Bahia, no período de outubro de 2003 a fevereiro de 2004. Utilizou-se o método de enxertia de fenda cheia, com o híbrido 'Hawaii 7996' como porta-enxerto e as cultivares Santa Clara, Santa Cruz Kada e Débora Plus, suscetíveis a R. solanacearum, como enxerto. O delineamento experimental foi em blocos ao acaso, com seis tratamentos (3 enxertados e 3 pés-francos), 5 repetições e 6 plantas por parcela útil. Avaliaram-se os caracteres: diâmetro do caule, altura da planta e da primeira inflorescência, distância entre inflorescências e produtividade comercial de frutos. O desenvolvimento das plantas, a produção total e a massa média dos frutos foram semelhantes para os tratamentos enxertados em relação a seus respectivos pés-francos e não houve incompatibilidade entre porta-enxerto e enxerto. A cultivar Santa Cruz Kada foi mais sensível às condições agroecológicas do município de Cruz das Almas, refletindo negativamente na produção e no peso médio dos frutos. Observou-se na enxertia com o híbrido 'Hawaii 7996' potencial para viabilizar a produção de tomateiros comerciais suscetíveis à murcha bacteriana, em áreas infestadas com R. solanacearum.