https://www.alice.cnptia.embrapa.br/alice/handle/item/41 Embrapa Agroenergia (CNPAE) 2026-08-09T14:39:49Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1188882 Título: Comparative phylogenomics and transcriptional regulatory networks of AQPs, HSPs, and LEA proteins in salt-stressed Portulaca oleracea. Autoria: SILVA, T. L. C. da; BRAGA, Í. de O.; BARBOSA, L. F.; LEAO, A. P.; SANTOS, W. R. dos; TOGAWA, R. C.; MARTINS, N. F.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. Conteúdo: Abstract: Soil salinization severely threatens global food security, necessitating systematic investigations of halophytes like Portulaca oleracea to decode the molecular mechanisms of environmental resilience. Utilizing an integrated framework of deep learning-based genome annotation (58,817 predicted genes; 96.5% BUSCO completeness), multi-tissue RNA-Seq, phylogenomics, and gene regulatory network (GRN) inference, the synergistic orchestration of 78 aquaporins (AQPs), 525 heat shock proteins (HSPs), and 119 late embryogenesis abundant (LEA) proteins was elucidated. The active transcriptome, encompassing 39,065 expressed loci, revealed a systemic growth-defense trade-off. Tissues displayed distinct adaptive mechanisms: leaves modulated intracellular water balance via specialized AQPs, whereas adult roots maintained proteostasis through robust HSP20/HSP70 induction. Phylogenomic clustering across 154 species demonstrated that salinity tolerance constitutes an evolutionary mosaic, identifying 81 halophyte-exclusive orthogroups and 1129 species-specific clusters. Comparative topology across six independent GRNs (4.2M–5.3 M edges) unmasked a highly modular transcriptional reprogramming strategy governed by a core apparatus of 22 stress-exclusive regulators, with functional enrichment heavily prioritizing protein dimerization and chromatin remodeling. Theoretically, the distinct convergence of Trihelix transcription factors with guard cell differentiation pathways offers a candidate transcriptomic framework to explain the plant's characteristic C4–CAM photosynthetic plasticity under severe osmotic pressure. Practically, these evolutionary blueprints and specific master switches transcend single-gene transgenic limitations. Utilizing these root-sustained and stress-inducible targets under localized promoters provides a naturally optimized, network-level precision engineering roadmap to transfer robust, compartmentalized halotolerance to sensitive glycophytic crops. 2026-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1188898 Título: Circular economy in agrifood RD&I: a global bibliometric review. Autoria: PENA JUNIOR, M. A. G.; ANJOS, S. S. N. dos; LEITÃO, F. O. Conteúdo: Abstract: This study examines how circular economy (CE) has been incorporated into the scientific domain of agrifood research, development, and innovation (RD&I), focusing on the conceptual structure, thematic evolution, and dominant innovation orientations that characterize this interface. Although CE has become increasingly prominent in sustainability debates, its integration into agrifood RD&I remains fragmented and uneven, particularly regarding the articulation between circularity principles, technological innovation, and regenerative systemic transformation. To address this gap, a structured bibliometric analysis was conducted using data retrieved from the Web of Science Core Collection, Scopus, CAB Abstracts, Dimensions, and FAO AGRIS databases. The final corpus comprised 1,456 journal articles published between 2023 and March 2026. Performance analysis and science mapping techniques were applied through the Bibliometrix package in R to examine keyword co-occurrence, thematic evolution, conceptual clustering, and collaboration patterns. The results show that the field is expanding rapidly but without a corresponding stabilization of its conceptual core. Circular economy operates as a broad integrative framework linking sustainability, waste management, biomass valorization, and life cycle assessment, while emerging technologies such as artificial intelligence and blockchain remain structurally secondary. This indicates that, despite their growing prominence in policy and strategic discourse, digital agriculture-related technologies have not yet become a central research front in circular economy-oriented agrifood RD&I, remaining substantially peripheral to the dominant scientific structure of the field. Biomass-related pathways and efficiency-oriented approaches dominate the current architecture of the literature, whereas broader regenerative and system-level transition perspectives remain comparatively underdeveloped. The findings indicate that CE has been incorporated into agrifood RD&I through a hybrid and asymmetric process in which sustainability framing, resource recovery, and technology-oriented innovation coexist without full conceptual integration. These results provide an empirical basis for advancing more coherent circular and regenerative agrifood RD&I agendas and for strengthening the integration between technological innovation, system-level transformation, and circularity principles. 2026-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1188836 Título: Sugarcane genetic transformation aiming drought tolerance. Autoria: DIAS, B. B. A.; YAMAGUCHI-SHINOZAKI, K.; MOLINARI, H. B. C. Conteúdo: The importance of sugarcane as an energy crop has grown in recent years given to one of its products, ethanol, seems as one of the most viable alternative to replace fossil fuels. In the case of sugarcane, stable transformation is not a routine step. Low transformation efficiency, transgene inactivation, somaclonal variation on tissue culture, and the long time required for regeneration and commercial release are some drawbacks facing the crop. 2011-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1188202 Título: Pd/Activated biocarbon applied to a glycerol acid-alkaline electroreformer for simultaneous H2 and electricity production. Autoria: LINO, F. M. de A.; GAMBETTA, R.; FAVARO, S. P.; LINARES, J. J. Conteúdo: Abstract: This manuscript proposes to valorize the macauba endocarp (E) wastes from the macauba processing by a pyrolytic + hydrothermal process to produce activated carbon (ACE), followed by chemical treatment with H2O2 (ACEA) and HNO3 (ACEB). Elemental analysis, surface area, pore-size distribution, and surface functional groups are investigated for the different prepared materials. ACEA and ACEB offer a higher surface area and functionality than Vulcan XC-72R (VC), making them suitable as carbon supports for Pd nanoparticles. The prepared Pd/ACEA and Pd/ACEB are physicochemically characterized by X-ray diffraction and Transmission Electron Microscopy. Their electrochemical performance is initially evaluated in a 3-electrode glass cell and is found to surpass that of Pd/VC in glycerol electro-oxidation. This trend is confirmed in the glycerol acid-alkaline electroreformer, where hydrogen and electricity were simultaneously produced, achieving a maximum power density of 0.28 kW m−2 and H2 flux of 0.6 STP m3 m−2 h−1 at 80 °C. 2026-01-01T00:00:00Z