https://www.alice.cnptia.embrapa.br/alice/handle/item/41 Embrapa Agroenergia (CNPAE) 2026-06-10T00:38:40Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187414 Title: Enhancing wheat bran deconstruction with enzyme cocktails from Penicillium spp. and Trichoderma harzianum: the impact of β-glucosidase supplementation. Authors: HAMANN, P. R. V.; MARTINS, H. S.; COSTA, P. H. C. da; BRITO, D. T. de A.; SILVA, V. R. C. da; TAVARES, Y. F.; QUIRINO, B. F.; NORONHA, E. F. Description: Abstract: Forests and natural environments are valuable sources of organisms capable of degrading plant cell wall carbohydrates. In the present study, two isolates, Penicillium polonicum and Penicillium chrysogenum, obtained from Brazilian Cerrado soil, along with a previously characterized isolate of Trichoderma harzianum, were evaluated for their ability to produce carbohydrate-active enzymes when grown in the presence of wheat bran. P. chrysogenum exhibited higher production of endoglucanases, xylanases, and pectinases. Biochemical characterization showed that most enzymes were active at pH 5 and within a temperature range of 30–50 °C, with the exception of P. chrysogenum endoglucanases, which displayed optimal activity at 60 °C. Hydrolysis of cellulose and carboxymethyl cellulose by the three enzyme sources demonstrated that supplementation with recombinant β-glucosidase from Clostridium thermocellum (BglA) significantly enhanced reducing sugar release; specifically, when applied to P. chrysogenum, the reducing sugar yield from cellulose hydrolysis increased by 2.53-fold. In the hydrolysis of wheat bran, enzymes from P. chrysogenum and T. harzianum supplemented with BglA resulted in a 1.6- and 3.93-fold increase in D-glucose yield, respectively. The results presented in this study contribute to the development of more robust enzyme cocktails for wheat bran valorization. 2026-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187275 Title: Obtenção, caracterização e avaliação de membranas poliméricas à base de lignina e amido termoplástico para separação de CO2. Authors: SOUZA, A. P. R. de; PASQUINI, D.; VAZ JUNIOR, S. Description: Resumo: A tecnologia de separação de gases através de membranas se mostra promissora diante da sua processabilidade e aplicação. Esse trabalho tem como objetivo obter e caracterizar membranas poliméricas renováveis (MPR) para separação de dióxido de carbono (CO2). A metodologia consiste em duas etapas: 1) oxipropilação de ligninas e 2) obtenção de MPR a partir da adição de amido termoplástico (ATP). FTIR, TGA e MEV foram realizadas nas MPR. Dióxido de titânio (TiO2) foi adicionado ao material visando a otimização dos parâmetros de permeação. As MPR sem TiO2 não se mostraram favoráveis à permeação de nitrogênio (N2) e CO2, enquanto as MPR com TiO2 apresentaram resultados promissores para a permeação de CO2. Diante disso, a utilização de TiO2 se faz essencial na composição das MPR. 2025-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187279 Title: A new GH3 B-Glucosidase from Chryseobacterium sp. with applications in cellulosic ethanol production and agri-biotechnological processes. Authors: BERGMANN, J. C.; LACERDA, V. A. M.; ALENCAR, K. L. C.; FAVARO, L. C. de L.; RODRIGUES, D. de S.; MARINS, L. F.; QUIRINO, B. F. Description: Abstract: β-Glucosidases catalyze the hydrolysis of β-glycosidic bonds and play key roles in biomass conversion and glycoside processing. We report the identification and characterization of Cr_B1, a GH3 β-glucosidase from Chryseobacterium sp. containing a predicted signal peptide. Cr_B1 hydrolyzed pNPG, cellobiose, salicin, and daidzin, showing optimal activity at pH 5.0−5.5 and 45−55 °C. The enzyme retained over 90% activity after 190 days at 4°C and 25 °C and above 80% activity after 24 h at 50 °C, indicating remarkable long-term and thermal stability. Cr_B1 exhibited high glucose tolerance (IC50: 1.5−1.8 M) and substrate-dependent kinetics. In synergy with Celluclast, it increased glucose release from CMC by 69%, demonstrating its potential to enhance enzymatic saccharification. These properties highlight Cr_B1 as a promising biocatalyst for improving saccharification, enhancing isoflavone bioavailability, and reducing bitterness in food and feed applications. 2026-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1186824 Title: Visualizando a difratometria. Authors: VALADARES, L. F.; VALADARES, N. F. Description: This classroom-oriented study goes beyond simple visualization by directly comparing laser diffractometry and light microscopy measurements to quantify micron-scale spacings in commercial CDs/DVDs (compact disc/digital versatile disc) and electron-microscopy grids. Diffraction angles from reflection (CD/DVD) and transmission (grids) patterns were captured using a digital camera. We systematically validate the diffractometry results with microscopy, teaching the scientific principle of cross-validation. Furthermore, by employing grids with square, rectangular, and hexagonal symmetries, we provide a tangible introduction to symmetry and reciprocal space, demonstrating the inverse relationship between grating spacing and diffraction pattern scale. Designed for educational settings, these experiments use affordable materials to teach core principles of diffraction with applications in material science and chemistry. 2026-01-01T00:00:00Z