DSpace Coleção: Artigo em periódico indexado (CNPC)

Reuse of progesterone device did not affect embryo yield in locally adapted Brazilian sheep.

Thu, 01 Jan 2026 00:00:00 GMT

Título: Reuse of progesterone device did not affect embryo yield in locally adapted Brazilian sheep. Autoria: FIGUEIRA, L. M.; LIMA, M. S. D.; VERGANI, G.; SILVA, K. de M.; MONTEIRO, A. W. U.; BATISTA, R. I. T. P.; TEIXEIRA, D. I. A.; OLIVEIRA, M. E. F.; FONSECA, J. F. da Conteúdo: Abstract: The reuse of progesterone devices is safe, efficient, and economically viable in small ruminants, supporting the evaluation of extended reuse protocols in superovulated ewes. Thus, the objective of this study was to test the efficiency of progesterone device reuse in superovulated ewes. Morada Nova (MN, n = 20) and Santa Inês (SI, n = 20) ewes were equally assigned to G-New (n = 20) and G-Used (n = 20) treatments. Ultrasound examinations in B- and Color Doppler modes were performed on DO (device insertion), D7, D9 (device removal), D11, and D15 to assess ovarian dynamics. Superovulation consisted of six decreasing doses of p FSH (133 mg total) administered intramuscularly at 12 h intervals, starting 60 h before device removal. On D16, nonsurgical embryo recovery (NSER) was performed in ewes presenting at least one corpus luteum (CL). The number of follicles after p FSH treatment was greater (P< 0.05) in SI ewes. A breed-by-treatment interaction (P < 0.05) was observed for the interval to estrus, number of corpora lutea, and luteinized structures. Notably, a slight delay in estrus onset and poorer superovulatory responses occurred only in G-New MN ewes (60% with CL and 4.7 ± 0.7 CL). However, they presented satisfactory numbers of CL and viable embryos per ewe flushed (7.8 ± 0.9 and 6.6 ± 1.1, respectively). The average total number of viable embryos in this trial was 9.2 ± 1.7. Positive correlations (P < 0.05) were found between ovarian blood perfusion on D15 and the number of luteinized structures/CL in G-New (r = 0.79) and G-Used (r = 0.54). However, correlations with recovered structures (r = 0.63) and viable embryos (r = 0.63) were observed only in G-New. In conclusion, reusing a progesterone device once for nine days (mid-term protocol) was as effective as using a new device in terms of embryo yield in locally adapted Brazilian ewes submitted to a superovulatory protocol and NSER. Resumo: A reutilização de dispositivos de progesterona é segura, eficiente e economicamente viável em pequenos ruminantes, o que respalda a avaliação de protocolos de reutilização estendida em ovelhas superovuladas. Assim, o objetivo deste estudo foi avaliar a eficiência da reutilização de dispositivos de progesterona em ovelhas superovuladas. Ovelhas das raças Morada Nova (MN, n = 20) e Santa Inês (SI, n = 20) foram igualmente distribuídas nos tratamentos G-Novo (n = 20) e G-Usado (n = 20). Exames ultrassonográficos nos modos B e Doppler colorido foram realizados nos dias D0 (inserção do dispositivo), D7, D9 (remoção do dispositivo), D11 e D15 para avaliar a dinâmica ovariana. A superovulação consistiu na administração de seis doses decrescentes de p-FSH (133 mg no total), por via intramuscular, em intervalos de 12 h, iniciando-se 60 h antes da remoção do dispositivo. No D16, realizou-se a recuperação não cirúrgica de embriões (RNCE) nas ovelhas que apresentaram pelo menos um CL. O número de folículos após o tratamento com p-FSH foi maior (p < 0,05) na raça SI. Houve interação raça x tratamento (p < 0,05) para o intervalo até o estro, número de corpos lúteos e estruturas luteinizadas. Observou-se discreto atraso no início do estro e menor resposta superovulatória apenas nas ovelhas MN do grupo G-Novo (60% com CL e 4,7 ± 0,7 CL), embora tenham apresentado números satisfatórios de CL e de embriões viáveis por ovelha submetida à lavagem uterina (7,8 ± 0,9 e 6,6 ± 1,1, respectivamente). O número médio total de embriões viáveis neste ensaio foi de 9,2 ± 1,7. Além disso, foram observadas correlações positivas (p < 0,05) entre a perfusão sanguínea ovariana no D15 e o número de estruturas luteinizadas/CL no G-Novo (r = 0,79) e no G-Usado (r = 0,54); contudo, apenas no G-Novo houve correlação com o número de estruturas recuperadas (r = 0,63) e de embriões viáveis (r = 0,63). Conclui-se que a reutilização do dispositivo de progesterona por nove dias (protocolo de médio prazo) apresentou eficácia equivalente à do dispositivo novo quanto à produção de embriões em ovelhas brasileiras localmente adaptadas submetidas a protocolo superovulatório e à recuperação não cirúrgica de embriões.

Modeling herbaceous biomass and assessing degradation risk in the Caatinga biome using Monte Carlo Simulation.

Wed, 01 Jan 2025 00:00:00 GMT

Título: Modeling herbaceous biomass and assessing degradation risk in the Caatinga biome using Monte Carlo Simulation. Autoria: FIGUEIREDO, J. R. de O.; PEREIRA FILHO, J. M.; FEITOSA, J. F. de F.; CÂNDIDO, M. J. D.; GILLES, S.; BAKKE, O. A.; MARANHÃO, S. R.; CAVALCANTE, A. C. R.; EDVAN, R. L.; BEZERRA, L. R. Conteúdo: Abstract: Simulating scenarios under climate change is essential to understanding vegetation dynamics, ensuring the survival of forage species, and minimizing uncertainties in project costs and timelines. This study aimed to simulate historical probabilities and develop a biomass production model using PHYGROW software (Texas A&M University, College Station, TX, USA), combined with Monte Carlo Simulation (MCS) in the @RISK program (Ithaca, NY, USA), to evaluate long-term biomass production in a native pasture area of the Caatinga biome. The results show strong agreement between software estimates and field data. For 2016, PHYGROW estimated 883 kg/ha, while field measurements reached 836.8 kg/ha; for 2017, 1117 kg/ha was estimated, while 992.15 kg/ha was observed. For 2018, the model estimated 1200 kg/ha compared with 1763.5 kg/ha in the field, and for 2019, 1230 kg/ha was estimated versus the 1294.3 kg/ha observed. The Monte Carlo simulations indicated that the Weibull distribution best fitted the synthetic series, with 90% adherence. Biomass production values ranged from 618 to 1427 kg/ha with a 90% probability. Only 5% of the simulations projected values below 600 kg/ha or above 1400 kg/ha. Moreover, there was a 95% risk of production issues if planning was based on biomass values above 1000 kg/ha. These findings highlight PHYGROW’s potential for pasture management under semi-arid conditions for predicting and avoiding degradation scenarios that could even lead to areas of desertification.

GHG production and energy partitioning in female and male Somali sheep under different diets.

Wed, 01 Jan 2025 00:00:00 GMT

Título: GHG production and energy partitioning in female and male Somali sheep under different diets. Autoria: ALVES, F. G. da S.; ROGERIO, M. C. P.; FERREIRA, A. L.; NEIVA, J. N. M.; POMPEU, R. C. F. F.; LIMA, L. D. de Conteúdo: Abstract: The objective of this work was to evaluate greenhouse gas (GHG) production and energy partitioning in growing female and male Brazilian Somali sheep subjected to different diets. The experiment was completely randomized, in a 3×2 (three diets combining crude protein and total digestible nutrients x sex classes) factorial arrangement, with four replicates. The factorial analysis of variance was used, and means were compared by Tukey’s test. GHG emissions and energy partitioning were determined using open-circuit respirometry chambers. No effect of sex was observed. Sheep fed the diet that met 100% of the recommendations of National Research Council (NRC) showed a higher gross energy intake, digestible energy intake, oxygen consumption, carbon dioxide production, heat production, and respiratory quotient than sheep fed the diet that met only 85%. However, both diets showed similar results for gross energy loss via feces, metabolizable energy intake, energy balance, metabolizability, metabolizable:digestible energy ratio, and daily methane production. The net energy requirement of Brazilian Somali sheep did not differ between sex classes during their early growth phase. At this phase, the diet that met 85% of the NRC recommendations can be used instead of the one that met 100%, without reducing energy efficiency or increasing methane emissions. RESUMO – O objetivo deste trabalho foi avaliar a produção de gases de efeito estufa (GEE) e a partição de energia em fêmeas e machos de ovinos da raça somalis brasileira, em crescimento, submetidos a diferentes dietas. O experimento foi em delineamento inteiramente casualizado, em esquema fatorial 3×2 (três dietas combinando proteína bruta e nutrientes digestíveis totais x sexos), com quatro repetições. Utilizou-se a análise de variância fatorial, e compararam-se as médias com o teste de Tukey. As emissões de GEE e a partição de energia foram determinadas com uso de câmaras de respirometria em circuito aberto. Não foi observado efeito do sexo. As ovelhas alimentadas com a dieta que atendia 100% das recomendações do National Research Council (NRC) apresentaram maior ingestão bruta de energia, ingestão de energia digestível, consumo de oxigênio, produção de dióxido de carbono, produção de calor e quociente respiratório do que as que receberam a dieta que atendia 85%. No entanto, ambas as dietas apresentaram resultados semelhantes para perda bruta de energia via fezes, ingestão de energia metabolizável, balanço energético, metabolizabilidade, relação energia metabolizável:energia digestível e produção diária de metano. A necessidade líquida de energia dos ovinos da raça somalis brasileira não diferiu entre os sexos durante a fase inicial de seu crescimento. Nessa fase, a dieta que atingiu 85% das recomendações do NRC pode ser utilizada em vez da que atingiu 100%, sem reduzir a eficiência energética ou aumentar as emissões de metano.

Genome-scale mining of simple sequence repeats (SSRs) in the forage grass Urochloa mosambicensis using low-coverage whole-genome sequencing data: abundance, distribution, and composition abundance, distribution, and composition.

Wed, 01 Jan 2025 00:00:00 GMT

Título: Genome-scale mining of simple sequence repeats (SSRs) in the forage grass Urochloa mosambicensis using low-coverage whole-genome sequencing data: abundance, distribution, and composition abundance, distribution, and composition. Autoria: LEÃO, E. S.; SILVA, G. R. da; BUENO, L. G.; NEGREIROS, A. B.; DINIZ, F. M. Conteúdo: Abstract: Simple sequence repeats (SSRs), also known as microsatellites, are common components of prokaryotic and eukaryotic genomes. Microsatellite loci are widely applied as molecular marker systems in plant population studies and genetic breeding due to their codominant inheritance, high polymorphism, and reproducibility. The development of these markers, however, requires prior genomic information. Although low-coverage whole-genome sequencing using the Illumina MiSeq platform provides limited sequencing depth, it is usually sufficient to identify thousands of SSR regions. In this study, low-coverage sequencing was performed on the Urochloa mosambicensis genome using the Illumina MiSeq platform. This tropical forage grass shows strong potential for adaptability and persistence in dry environments, making it a promising feed source for ruminants in harsh conditions. The U. mosambicensis genome was screened for SSRs to evaluate their potential for molecular marker development. The high-quality Illumina sequencing reads were assembled into 32,931 contigs (N50 = 395 bp) using the CLC Genomics Workbench. The software Krait and MSDB were used to analyze the low-coverage U. mosambicensis sequencing data in order to identify and evaluate pure, interrupted pure, compound, and interrupted compound SSRs. A total of 2,665 pure microsatellites were identified, with the highest relative abundances found in trinucleotides (31.52 loci/Mb) and pentanucleotides (16.62 loci/Mb). Hexanucleotides (87.18%) and trinucleotides (86.96%) exhibited the highest proportion of sequences suitable for amplification. A greater abundance of interrupted compound motifs (1.81 loci/Mb) and pure compound SSRs (1.15 loci/Mb) was observed compared to interrupted pure SSRs (0.48 loci/Mb). This study also suggests that non-consensus bases positively influence the size of interrupted pure microsatellites, indicating greater stability against potential mutations. These findings provide a valuable resource for the selection of potential molecular markers for genetic breeding and population studies of the tropical forage species U. mosambicensis.