https://www.alice.cnptia.embrapa.br/alice/handle/item/160 Artigo em periódico indexado (CNPGC) Thu, 21 May 2026 16:56:30 GMT 2026-05-21T16:56:30Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1186955 Título: Emergence of distinct Burkholderia pseudomallei lineages in Brazil: Phenotypic, genotypic, and phylogenetic agreement. Autoria: VELOSO, D. S.; ARAUJO, F. R.; SANTOS, L. R. dos; SUNIGA, P. A. P.; MORIYA, J. C. K.; VERBISCK, N. V.; GASQUÉ, M.; LAROUCAU, K.; LEMOS, E. R. S.; HORTA, M. A. Conteúdo: ABSTRACT - Burkholderia pseudomallei is a Gram-negative facultative intracellular bacterium that causes melioidosis, a neglected tropical disease that spreads beyond traditional endemic regions. This study examined B. pseudomallei isolates obtained from five melioidosis cases in Piauí, Brazil, using phenotypic, molecular, and genomic methods. Isolates were identified as B. pseudomallei using VITEK 2 system and MALDI-TOF mass spectrometry. Conventional PCR targeting the orf11, burk17, and burk475 gene regions confirmed species-level identification, with sequences showing 98.78–100% identity to GenBank B. pseudomallei references. Whole-genome sequencing and multi-locus sequence typing (MLST) revealed two novel sequence types; ST2226 (four isolates), clustering closely with strains from Cear´ a (ST1455 and ST1458), ST2234 (one isolate), related to previously reported ST95 and ST1355 strains, previously detected in Brazil. Phylogenetic analysis based on single nucleotide polymorphism (SNPs) and concatenated MLST data showed clear genetic distinction between the two lineages, suggesting regional spread or shared ancestry. These findings confirm the presence of at least two distinct genetic B. pseudomallei lineages in Piauí, providing insights into bacterial diversity and regional epidemiology in Brazil and reinforcing the importance of molecular surveillance and improved diagnostic capacity for melioidosis. Thu, 01 Jan 2026 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1186955 2026-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1186958 Título: Meat species identification and classification by MALDI-TOF mass spectrometry. Autoria: VERBISCK, N. V.; SOUZA, L. B. de; CARDOZO, M. V.; GUIMARAES, N. G. P.; FEIJO, G. L. D. Conteúdo: ABTRACT - Protein profiling generated by MALDI-TOF (matrix-assisted laser desorption ionizationtime-of-flight) was used to distinguish meat from different livestock species and identify meat species. Meat proteins from fresh beef (n = 12), pork (n = 7), chicken (n = 5) and tilapia fish (n = 3) were extracted and analyzed in a MALDI Biotyper mass spectrometer (Bruker Daltonics, Bremen, Germany) with alpha-cyano-4-hydroxycinnamic acid as the matrix. Mass spectra allowed the distinction of meat species, including between Nellore and Angus bovine breeds, and PCA classification revealed possible biomarkers for meat types. Our results corroborate MALDI-TOF mass spectrometry as an interesting tool for meat identification, which is useful for quality control and the certification of meat products. Thu, 01 Jan 2026 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1186958 2026-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1186361 Título: Nutrition management strategies for the adaptation of cull beef heifers to feedlot. Autoria: SILVA, M. G. P. da; ÍTAVO, L. C. V.; GOMES, R. da C.; ÍTAVO, C. C. B. F.; ARCANJO, A. H. M.; SANTANA, J. C. S.; TEIXEIRA, P. D.; GURGEL, A. L. C. Conteúdo: ABSTRACT - Providing diets with high roughage in the first days of confinement is the common way of adapting beef cattle to intensive production. However, it is possible to offer the final finishing diet from the first day of adaptation. Our objective was to compare a traditional adaptation protocol, in which animals received a diet with a higher proportion of roughage, with an experimental protocol, in which animals received the final finishing diet in restricted amounts, on the productive performance of beef heifers in the first 42 days ofconfinement, divided into two periods (0–14 days, 15–42 days). A total of 552 heifers were evaluated and subjected to 2 adaptation strategies: (1) traditional protocol—ad libitum feed with a quantified greater proportion of roughage in the first 14 days, and (2)experimental protocol—gradual increase in the amount of final finishing feed. The nutrient intake and productive performance were evaluated. The intake was greater in the traditional protocol in all periods, 8.2 vs. 7.9 kg/day, 9.6 vs. 9.2 kg/day, and 9.2 vs. 8.8 kg/day,to 0–14, 15−42, and 0–42 days, respectively. However, performance was better in the experimental protocol in the initial period(0–14 days), 0.7 vs. 0.3 kg/day. After adaptation (15–42 days), the traditional protocol performed better, 1.5 vs. 1.3 kg/day. The nutritional protocol of gradual adaptation to total feed is recommended for heifers adapting to confinement. Thu, 01 Jan 2026 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1186361 2026-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1184738 Título: Management practices for the control of Haematobia irritans, Dermatobia hominis, and Cochliomyia hominivorax in cattle across Latin America: a sustainable, collective approach. Autoria: RODRIGUEZ-VIVAS, R. I.; CUTOLO, A. A.; BARROS, A. T. M. de; CUORE, U. D.; MOLENTO, M. B.; LÓPEZ-OSORIO, S.; RODRIGUES, D. S.; SPINA, M.; BORGES, F. A.; LOPES, W. D. Z.; PULIDO-MEDELLIN, M. O.; FIEL, C. A.; COSTA-JUNIOR, L. M.; ANZIANI, O. S.; SAN MARTÍN, L. M.; SABATINI, G. A. Conteúdo: ABSTRACT - Bovines are suitable hosts and can be affected by fly infestations. Flies pose a significant threat to cattle livestock in Latin America (LA), causing substantial economic repercussions to animal production (reduced productivity, veterinary expenses, and decreased animal welfare) and damage to human health. The most important flies affecting cattle in Argentina, Brazil, Colombia, Mexico, and Uruguay are Haematobia irritans, Dermatobia hominis, and Cochliomyia hominivorax. Due to production losses and the consequent economic costs associated with these flies, control measures must be implemented, primarily relying on insecticidal products. However, decision-making for preventing and treating animals with insecticides varies due to differences in environmental conditions across countries and regions, production systems, animal populations, infestation levels, animal welfare, and the prevalence of myiasis, among other factors. Although insecticides remain the most effective option for fly control in cattle, resistant populations have developed, rendering them less effective. To overcome fly resistance to insecticides, non-chemical (mechanical, environmental, biological, and genetic) methods are being integrated into alternative control and eradication strategies. The use of integrated livestock fly control contributes to safeguarding animal, public, and environmental health. This review is designed to support individuals and institutions, both civil and governmental, addressing the ongoing challenge posed by flies affecting livestock. Thu, 01 Jan 2026 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1184738 2026-01-01T00:00:00Z