https://www.alice.cnptia.embrapa.br/alice/handle/item/172 Nota Técnica/Nota científica (CNPGL) Mon, 13 Apr 2026 23:14:52 GMT 2026-04-13T23:14:52Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1178364 Título: A respirometry system designed for small ruminants. Autoria: SILVA, C. S.; DIAVÃO, J.; MOTTA, E. F.; SILVA, A. S.; TONUCCI, R. G.; TOMICH, T. R.; MACHADO, F. S.; CAMPOS, M. M. Conteúdo: This technical note describes a small ruminant respiration chamber system designed to accurately quantify the production of carbon dioxide (CO2) and methane (CH4). The system consists of 3 open-circuit respiration chambers, flow meters, gas analyzers, and an accessible environmental control system. To validate its performance, gas recovery tests were conducted by injecting CO2 and CH4 at 4 constant flow rates: 0.4, 0.8, 1.2, and 1.6 standard liters per minute (SLPM) for CO2, and 0.16, 0.20, 0.24, and 0.28 SLPM for CH4. Each injection level was tested across the 3 chambers. The average percentage recoveries for CO2 and CH4 were 97.8% ± 1.46% for chamber 1, 101.1% ± 1.49% for chamber 2, and 104.1% ± 1.98% for chamber 3 and 98.7% ± 0.97% for chamber 1, 99.4% ± 2.06% for chamber 2, and 101.6% ± 2.90% for chamber 3, respectively, demonstrating the system’s accuracy in quantifying livestock GHG emissions from small ruminants, such as young cattle, goats, and sheep. Therefore, this system represents a viable methodology for nutritional and environmental studies with small ruminants. Future studies conducted in this facility can contribute to a better understanding of the dynamic and flow of GHG emissions in small ruminants and target measures to increase the sustainability of small ruminant production systems. Wed, 01 Jan 2025 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1178364 2025-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1168458 Título: Screening pre-emergence herbicides for weed control during early elephant grass growth. Autoria: BRIGHENTI, A. M. Conteúdo: ABSTRACT- Two experiments were carried out in the municipality of Coronel Pacheco, Minas Gerais State, Brazil in 2020 and 2021 to identify additional herbicide options for weed control during early elephant grass pasture establishment. Thirteen pre-emergence herbicides were compared to weed-free and weedy controls in a randomized complete block design with 4 replications. Forage yield losses were significant as a result of weed interference throughout the entire crop cycle. The most phytotoxic treatments were trifluralin and diuron + hexazinone. Weed control was effective for all treatments, except for trifluralin applied alone. Elephant grass dry matter yield was not influenced by diuron, ametryne, flumioxazin and metribuzin, identifying them as potential pre-emergence herbicides for weed control in elephant grass pastures. RESUMEN- Se realizaron dos experimentos en el município de Coronel Pacheco, Estado de Minas Gerais, Brasil, el 17 de marzo de 2020 (experimento 1) y el 26 de febrero de 2021 para identificar opciones adicionales de herbicidas para el control de malezas en el desarrollo temprano del pasto elefante. El diseño experimental fue de bloques completos al azar, con cuatro repeticiones. Se compararon trece herbicidas preemergentes versus tratamientos control desmalezado y enmalezado. Las pérdidas en el rendimiento del forraje fueron significativas como resultado de la interferencia de malezas durante todo el ciclo de cultivo. Los tratamientos más fitotóxicos fueron trifluralina y diurón + hexazinona. El control de malezas fue efectivo para todos los tratamientos, excepto para la aplicación de trifluralina sola. El redimiento de materia seca de pasto elefante no fue influenciado por el diurón, ametrina, flumioxazina y metribuzina, siendo estos herbicidas preemergentes de uso potencial para el control de malezas en pasto elefeante. Mon, 01 Jan 2024 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1168458 2024-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1148220 Título: Development of microsatellite panels for molecular fingerprinting of Napier grass (Cenchrus purpureus) cultivars. Autoria: AZEVEDO, A. L. S.; SOUZA, F. R. de; CAMPOS, R. A.; REIS, D. R. de L.; MACHADO, J. C.; MACHADO, M. A.; LEDO, F. J. da S.; RESENDE, M. Conteúdo: Napier grass is a perennial tropical forage that is used in beef and dairy production systems. Despite its significance in animal nutrition, molecular information available, such as microsatellite or simple sequence repeat (SSR) or single nucleotide polymorphism (SNP) markers, is limited. Using an assembled transcriptome, 50 novel SSR markers were developed, of which 21 were found to be polymorphic. These polymorphic markers were tested for DNA fingerprinting of Embrapa cultivars, five of which revealed distinct allele patterns for cultivar identification. SSR markers 05, 17, and 44 identified a unique pattern in the BRS Kurumi cultivar. The BRS Capiaçu cultivar was identified using SSR markers 17, 43, and 44. The Pioneiro cultivar exhibited a rare fragment amplification pattern using SSR marker 46, while SSR marker 44 revealed a distinct allele in the BRS Canará cultivar. SSR marker panels could be utilized as DNA fingerprinting tools to assist in cultivar identification. Sat, 01 Jan 2022 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1148220 2022-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1134616 Título: Lentivirus vectors fail to deliver transgenes into bovine zygotes after co-incubation with sperm during in vitro fertilization. Autoria: LEMOS, D. R.; SOUZA, G. T.; SOUZA, V. G. P.; RIBEIRO, L. S.; SARAIVA, N. Z.; QUINTAO, C. C. R.; CAMARGO, L. S. de A. Fri, 01 Jan 2021 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1134616 2021-01-01T00:00:00Z