https://www.alice.cnptia.embrapa.br/alice/handle/item/205 Artigo em periódico indexado (CNPDIA) Thu, 11 Jun 2026 21:30:52 GMT 2026-06-11T21:30:52Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187436 Título: Natural deep eutectic solvent-assisted incorporation of anthocyanins into cellulose acetate nanofibers for halochromic sensors. Autoria: BERTOLO-CAGNOTO, M. R. V.; ALVARENGA, A. D.; CAMARGO, L. A. de; COSTA, K. B. T.; CORREA, D. S. Conteúdo: Intelligent food packaging can help reduce food waste by providing consumers real-time information about product freshness. They can be produced using natural pigments with pH-dependent color changes, which makes them promising candidates for colorimetric sensors. This study reports the development, through solution blow spinning (SBS), of cellulose acetate (CA)-based nanofibers loaded with anthocyanins solubilized in natural deep eutectic solvents (NADES). CA nanofibers were obtained by SBS using CA/PEO (polyethylene oxide) co-solutions, followed by PEO removal. Anthocyanins from grape pomace (GP), sweet potato (SP), and black carrot (BC) were incorporated into pectin solutions solubilized in a choline chloride–glycerol NADES (CC-GLY), which were then used to modify the fibrous membranes by immersion, yielding halochromic sensors. These sensors were char acterized regarding their morphological, thermal, and water-related properties. Anthocyanins incorporation decreased CA onset degradation temperatures and increased its melting enthalpy, suggesting improved chain packing. The fibers containing anthocyanins were more hydrophilic and more soluble. Exposure to ammonia vapor produced visible colorimetric responses, with CA/BC achieving the highest ΔE values (24.60 ± 0.50) and a detection limit as low as 1.73 × 10 6 mol L 1 . Finally, a proof-of-concept assay confirmed CA/BC applicability for monitoring fish (salmon) spoilage via headspace exposure (ΔE = 26.52 ± 0.97 after 144 h at 4 ◦ 28.49 ± 1.04 after 48 h at 25 ◦ C and ΔE = C). The results demonstrate that CA nanofibers doped with anthocyanins are promising natural materials for intelligent packaging applications, enabling rapid and sensitive detection of food spoilage. Thu, 01 Jan 2026 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187436 2026-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187455 Título: Selective electrochemical CO2 reduction to formate at industrial current densities using 2D Bi2O2CO3 nanosheets. Autoria: TOMAS, M. J.; SEVENE, D. R.; MARRENJO, G. J.; SILVA, C. P. M.; OLIVEIRA, J. A. de; RIBEIRO, C.; LOPES, O. F. Conteúdo: Electrochemical CO2 reduction to formate (HCOO-) represents an efficient strategy to mitigate CO2 emissions while producing value-added chemicals. However, the reaction typically requires high overpotential and suffers from low selectivity and current density. This work reports the development of Bi2O2CO3 (BOC) nanosheets synthesized hydrothermally with urea at 90 ◦C (BOC-90) and 150 ◦C (BOC-150), evaluated in a flow-cell configuration with gas diffusion electrodes. A Bi2O2CO3 sample calcined at 400 ◦C (Bi2O3–400) was also investigated for comparison. Both BOC-90 and BOC-150 outperformed Bi2O3–400 and a commercial BOC sample in HCOO- production. Notably, BOC-150 achieved a current density of -140 mA cm-2 and a Faradaic efficiency (FE) of 95% for HCOO- at -1.2 V vs. RHE in 0.5 M KOH. Optimizing the electrolyte concentration (1.0 M KOH) and electrode composition (75% BOC-150, 25% carbon black) further enhanced performance, yielding -250 mA cm-2 with FE for HCOO- (FEHCOO-) above 90%. EIS showed that BOC-150 has the lowest charge-transfer resis tance, while ECSA measurements confirmed its highest active surface area, jointly explaining its enhanced ECR performance. NIR and XPS analyses revealed that strongly adsorbed water and Bi dissolution reduced BOC-90 stability, while BOC-150 maintained structural integrity and favorable surface composition. The electrode exhibited stable operation for 6 h, maintaining a Faradaic efficiency above 80% throughout the electrolysis. These findings demonstrate that BOC nanosheets, particularly those obtained under optimized hydrothermal conditions, are highly efficient and durable electrocatalysts for CO2 reduction to HCOO- under industrially relevant conditions. Thu, 01 Jan 2026 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187455 2026-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187457 Título: Efficiency of Imazapic Degradation: an Assessment of LacMeta Treatments Utilizing Whole Cell. Autoria: LIMA, N. S. M.; GOMES-PEPE, E. S.; KOCK, F. V. C.; COLNAGO, L. A.; ALVES, P. L. da C. A.; LEMOS, E. G. de M. Conteúdo: The extensive use of herbicides such as imazapic, from the imidazolinone class, raises environmental concerns due to its persistence and toxicity in ecosystems and subsequent crops. Enzymatic bioremediation emerges as a sustainable alterna tive for the mitigation of these contaminants. This study investigated the potential of the metagenomic laccase, LacMeta, expressed in Escherichia coli BL21 (DE3), to degrade imazapic using a whole-cell approach. LacMeta expression was optimized with CuSO₄, which proved to be four times more effective than IPTG. The E. coli + LacMeta cells demonstrated high tolerance to the herbicide, maintaining cell viability even at high doses (350 g/ha). Notably, the enzymatic activity of LacMeta was not inhibited by imazapic; on the contrary, it was stimulated, reaching a specific activity nearly three times higher in the presence of the herbicide compared to the control. Degradation was confirmed by UV-Visible spec troscopy, which showed the disappearance of imazapic’s characteristic peaks (200–280 nm) over 15 days. ¹H-NMR and FTIR analyses corroborated the degradation, indicating structural changes in the herbicide molecule, particularly in the aromatic ring region (signals at 8.25 and 8.50 ppm). Phytotoxicity assays with lettuce seeds (Lactuca sativa) confirmed that treatment with the LacMeta-containing supernatant cell free significantly reduced the toxicity of imazapic in the soil. The results demonstrate that LacMeta has high potential for the bioremediation of imazapic, and the whole-cell approach represents a promising and cost-effective strategy for the decontamination of environments impacted by this herbicide Thu, 01 Jan 2026 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187457 2026-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187460 Título: Ucuúba Fat (Virola surinamensis) and Cellulose Nanocrystals Functionalizing Cassava (Manihot esculenta) Starch Films. Autoria: RODRIGUES, G. C. P.; PROCOPIO, F. R.; HENRIQUE, J. F.; BREXÓ, R. P.; FERREIRA, M. D. Conteúdo: Foodloss remainsamajorchallengealongtheproductionchain.Plant-basedediblecoatingshaveemergedasaneffective, sustainablestrategytoextendshelflife.Starch-basedcoatingshavedesirableopticalproperties,buttheirhighhydrophilicitylimits performance. IncorporatingnaturaladditivesfromAmazondiversity, suchaslipidsandcellulosenanotechnologyderivatives, canenhance their functionality. Themaingoalwas todevelopedible coatings fromBrazilian flora composedof cassava (Manihotesculenta)starch(CS)andUcuúba(Virolasurinamensis)(Ucu)fat,combinedwithcellulosenanocrystals(CNC).Eight formulationswerepreparedbycasting,varyingglycerol(25%and30%)andadditivecomposition(Ucu,CNC,andUcu+CNC). Theresultingfilmswerecharacterizedforphysical-chemical,optical,mechanical,andmicrostructuralproperties.Ucuúbafat increasedopacity, reducingglossandmechanicalstrength.CNC, incontrast,enhancedbrightnessandtensilestrength.Films withbothUcuandCNCshowedreducedwatercontactangle(WCA),suggestingincreasedwettability.TheyalsoimprovedUV Vislightblockingby60%comparedtocontrols.Microscopyrevealedthatcombinedfilmshadamorecontinuousandregular structurethanthosewithonlyUcuúbafat.TheseresultsdemonstratethatcombiningUcuandCNCcantailorthepropertiesof starch-basedcoatings.Thisapproachenablesthedevelopmentofsustainable,plant-derivedediblefilmstoprotectfreshfoods Thu, 01 Jan 2026 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187460 2026-01-01T00:00:00Z