https://www.alice.cnptia.embrapa.br/alice/handle/item/253 Nota Técnica/Nota científica (CNPMS) Wed, 08 Apr 2026 23:33:16 GMT 2026-04-08T23:33:16Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1155039 Título: Comparison of protocols for chromosome duplication in haploid maize. Autoria: AZEVEDO, T. C.; GUIMARAES, F. F. M.; TRINDADE, R. dos S.; LANA, U. G. de P.; SOUZA, I. R. P. de Conteúdo: Different methods of chromosome duplication are used to obtain doubled haploids in maize; however, comparisons of efficiency among protocols have only begun to appear in the literature. Therefore, this study aimed to compare the efficiency of three protocols of chromosome duplication. A randomized block experimental design was used, with three replications composed of three treatments of chromosome duplication (duplication by colchicine injection in plants, duplication by immersion of roots in colchicine solution, and duplication by immersion of newly germinated seedlings in colchicine solution). Two hundred seventy haploid seeds were obtained from crossing the genotype source 91500212 with the haploid inducer hybrid Tail P1 × Tail P2, with 90 seeds for each treatment. In the chromosome duplication through injection, a colchicine solution at 0.125% was applied to the stem of the seedlings 15 days after germination. In the chromosome duplication through root immersion, 15 days after germination, the roots of the seedlings were immersed in a 1% colchicine solution for six hours, followed by removing colchicine residues and transplanting them in trays with the substrate. Finally, in the chromosome duplication of newly germinated seedlings, three days old seedlings were immersed in a 0.06% colchicine solution for 12 hours, then removed from the solution and transplanted in trays with the substrate. Twenty days after treatment, the plants were transplanted in 20-liter pots with fertilized soil in a greenhouse, beginning data collection. Results indicate that the injection protocol showed better survival, better practicality of application, and lower costs and risks. Sun, 01 Jan 2023 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1155039 2023-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1154201 Título: State-of-the-art molecular plant sciences in Brazil. Autoria: REIS, P. A. B. dos; MAGALHAES, J. V. de; MILLER, R. N. G.; FONTES, E. P. B. Sun, 01 Jan 2023 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1154201 2023-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1144591 Título: Effect of earworm injuries on fumonisins production in Bt and non-Bt maize. Autoria: MENDES, S. M.; PIMENTEL, M. A. G.; QUEIROZ, V. A. V.; SILVA, D. D. da; COSTA, R. V. da Conteúdo: ABSTRACT - Tests were carried out to assess fumonisins production due to lepidopteran feeding on the ears of the hybrid DKB 390VTPRO (Cry1A105/Cry2Ab2) and in its non-Bt isogenic version. Maize ears were harvested and classified according to injury size, and fumonisin levels for each injury class were quantified. There were no significant differences between Bt maize and non-Bt maize in fumonisin production. However, the injury size due to earworm feeding was significant for fumonisin occurrence. The greater the injury, the higher the fumonisins level. RESUMO - Para avaliar a produção de fumonisinas causado pela alimentação de lepidopteros-praga nas espigas de milho Bt e não Bt, foram conduzidos testes, utlizando o híbrido de milho transgênico DKB 390VTPRO (Cry1A105/Cry2Ab2) e sua versão isogênica não-Bt (DKB 390). As espigas foram colhidas e divididas em quatro classes, quanto ao tamanho das injúrias e quantificados os teores de fumonisinas para cada classe. Não se verificou diferença significativa entre o milho Bt e sua versão não Bt, quanto ao níveis de ocorrência de fumonisinas. Contudo, houve efeito do tamanho da injuria na ocorrência de fumonisinas sendo que, quanto maior injúria, maior o nível de fumonisina. Sat, 01 Jan 2022 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1144591 2022-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1141161 Título: BRS Deni: super-sweet corn open-pollinated variety. Autoria: TEIXEIRA, F. F.; PEREIRA FILHO, I. A.; MEIRELLES, W. F.; MACHADO, J. R. de A.; PORTUGAL, A. F.; COTA, L. V. Conteúdo: BRS Deni is a super-sweet corn open-pollinated variety, developed and released by the Brazilian Agricultural Research Corporation (Embrapa) that combines high yield and good ear and grain quality and lower production costs. The grain can be consumed fresh or canned. Sat, 01 Jan 2022 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1141161 2022-01-01T00:00:00Z