https://www.alice.cnptia.embrapa.br/alice/handle/item/295 Artigo em periódico indexado (CENARGEN) Wed, 17 Jun 2026 02:55:22 GMT 2026-06-17T02:55:22Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187553 Título: An annotated list of viruses infecting or associated with passion fruit (Passiflora spp.). Autoria: VIDAL, A. H.; KITAJIMA, E. W.; RIBEIRO, S. da G. Conteúdo: Plants belonging to the genus Passiflora (family Passifloraceae) are found in tropical regions and are cultivated worldwide. Among them, the yellow passion fruit (P. edulis f. flavicarpa Deg.) is the most economically important species. This fruit is widely consumed fresh and is used in the juice industry. Additionally, it is employed as a natural ingredient in cosmetic and medicinal products. The yellow passion fruit is also grown as an ornamental plant in gardens and landscaping. One of the primary challenges in passion fruit cultivation is the occurrence of viral diseases, which can decrease both fruit production and quality, posing an ongoing threat to growers. Efficient strategies for managing and controlling viruses in these crops, particularly those that focus on developing resistant genotypes, rely on an extensive understanding of the viral diversity affecting the crop. In recent years, advances in technology and the development of new tools for virus detection and identification have led to a significant increase in the number of viruses reported to infect or be associated with Passiflora species and passion fruit hybrids. This review catalogs the known diversity of DNA and RNA viruses affecting the passion fruit crop. To date, this diversity includes 74 species (accepted and proposed) across 17 families and 25 genera of viruses. Thu, 01 Jan 2026 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187553 2026-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187409 Título: Genetic structure and diversity of Gliricidia sepium in Brazil: implications for conservation and breeding. Autoria: CARVALHO, A. M. de; SOUZA, J. L.; RANGEL, J. H. A.; SOUZA, S. F. de; MUNIZ, E. N.; SILVA, A. V. C. da Conteúdo: Gliricidia sepium is a tree species native to the Americas that has been disseminated throughout various regions of the world due to its drought adaptability. In response to the productive sector's demand for the development of cultivars, Empresa Brasileira de Pesquisa Agropecuária (Embrapa) has invested in breeding initiatives. These include establishing a germplasm bank and identifying individuals with agronomic traits of interest. This study aimed to estimate the genetic diversity of gliricidia in the different regions of Brazil and to establish a germplasm bank using inter simple sequence repeat (ISSR) markers. Plant material was obtained through field collections and exchanges with other institutions from 17 populations from five Brazilian states, and young leaves were collected from a total of 115 individuals. The ISSR markers yielded 36 bands with 88.9 % polymorphism and an average polymorphism information content (PIC) of 0.27. The evaluated parameters indicated low genetic diversity, with a Shannon index (I) of 0.19, Nei's Genetic Distance of 0.21, and an expected heterozygosity (He) of 0.13. A unweighted pair group method with arithmetic mean (UPGMA) analysis clustered the individuals into six groups, indicating a restricted origin for the gliricidia genetic material. Genetic structure analysis using the Structure software indicated the formation of two main groups, along with individuals with mixed ancestry, suggesting partial genetic admixture between the groups. The results of this study will guide the breeding program for gliricidia, with the germplasm bank continuously evaluated and enriched with new accessions. Thu, 01 Jan 2026 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187409 2026-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187303 Título: Computer-aided design of Plasmodium chabaudi-derived peptides with dual antibiofilm and anti-inflammatory activities. Autoria: OROZCO, R. M. Q.; SANTOS, A. D. O.; ROSSETTO, J. P.; MADALOZZO, E. S.; LUCHI, L. V.; REZENDE, S. B.; CHAN, L. Y.; BUCCINI, D. F.; MACEDO, M. L. R.; REIS, A. M. dos; CRAIK, D. J.; FRANCO, O. L.; CARDOSO, M. H. Conteúdo: Advances in computational design have greatly accelerated antimicrobial peptide engineering. In this study, three Plasmodium chabaudi-derived peptides (PcDBS1R1, PcDBS1R5, and PcDBS1R9), generated using the Joker computational design algorithm, were synthesized and characterized for their structural and functional properties. Biophysical analyses revealed that PcDBS1R5 and PcDBS1R9 predominantly adopted α-helical structures with high amphipathicity, whereas PcDBS1R1 exhibited greater structural plasticity. PcDBS1R5 and PcDBS1R9 displayed antibacterial activity against an Acinetobacter baumannii clinical isolate, whereas PcDBS1R1 showed pronounced antibiofilm effects. None of the peptides exhibited cytotoxicity toward murine macrophages, and all significantly reduced nitric oxide production in lipopolysaccharide-stimulated macrophages, suggesting potential anti-inflammatory activity. Overall, these findings demonstrate that computer-aided design of P. chabaudi-derived peptides can yield molecules with antibiofilm, and immunomodulatory properties, minimal cytotoxicity, and promising therapeutic potential as scaffolds for next-generation peptide-based treatments targeting biofilm-associated bacterial infections. Thu, 01 Jan 2026 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187303 2026-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187265 Título: Parity and season affect the performance of an automated activity monitoring system for estrus detection in grazing girolando cows. Autoria: OLIVEIRA, S. A. S. de; FIGUEIREDO, R. A.; VIANA, J. H. M.; FÉRES, L. L. dos S.; FÉRES, L. F. R.; SANTOS, R. M. dos; MARTINS, C. F.; FERREIRA, I. C. Conteúdo: Accurate estrus detection in grazing crossbred dairy cows (Zebu × Taurine) remains challenging in Brazil, and automated activity monitoring (AAM) systems may be a viable alternative. This study evaluated the effects of management and environmental factors on AAM performance for estrus detection in grazing Girolando cows. Nonpregnant 5/8 Gir × Holstein cows (n = 25) were monitored during both dry and rainy seasons. During the dry season, cows were managed in semiconfinement with corn silage supplementation. In the rainy season, they grazed Panicum maximum cv. Mombaça pastures with or without eucalyptus trees. Each cow wore an AAM collar (CowMed, Brazil), and after individual baseline calibration, cows followed a timed artificial insemination (TAI) protocol. A statistical model included season, location, parity, and detection method (AAM, visual observation [VO], adhesive patches [APs]) as fixed effects. Cow and cow × season interaction were random effects. Multiparous cows had higher estrus detection probabilities (97%) compared to primiparous cows (85%), regardless of method, location, or season. AAM detection probability was higher during the rainy season (98%) than during the dry season (73%). For AP, the detection probability was 80% in the rainy season and 97% in the dry season. VO detection remained stable at 92% in the dry season and 95% in the rainy season. Based on corpus luteum (CL) presence, AAM sensitivity and accuracy were 85% and 75%, respectively, while specificity was 33%. Parity and seasonal management significantly influenced AAM performance. The generally low specificity across detection methods highlights the need for improved algorithms and integrated estrus detection strategies in grazing systems. Thu, 01 Jan 2026 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187265 2026-01-01T00:00:00Z