https://www.alice.cnptia.embrapa.br/alice/handle/item/30 Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) Tue, 11 Aug 2026 20:41:02 GMT 2026-08-11T20:41:02Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1189084 Título: A comprehensive review on food-grade electrospinning of natural biopolymers for cultivated meat applications. Autoria: SILVA, N. M. A. da; SILVA, L. P. da Conteúdo: The production of cultivated meat relies on in vitro animal cell growth and requires the use of scaffolds that structurally resemble key features of the extracellular matrix (ECM), providing mechanical support and biochemical cues for cell adhesion, proliferation, and differentiation. Electrospinning has emerged as a promising technique for manufacturing three-dimensional edible scaffolds because it is robust, versatile, and capable of producing nanofibers with a high surface area-to-volume ratio, tunable porosity, and ECM-like fibrous architectures. Natural biopolymers are promising candidates for the fabrication of electrospun scaffolds, combining biocompatibility, biodegradability, and processing compatibility with food-grade requirements. However, the absence of fully food-grade electrospinning systems, coupled with limited scalable green-processing strategies, remains a critical barrier to industrial translation. In this context, this review presents recent advances in the food-grade electrospinning of natural biopolymers focused on cultivated meat production. Furthermore, scientific gaps in the development of fully edible scaffolds are discussed, along with the need for alternatives to animal-derived materials and synthetic carrier polymers, considering sustainability, consumer acceptance, and the translation from laboratory-scale studies to industrial systems. Finally, this review outlines a strategic roadmap to accelerate the transition from proof-of-concept studies toward scalable, regulatory-compliant, and industrially viable electrospinning technologies for cultivated meat production. Thu, 01 Jan 2026 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1189084 2026-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1189040 Título: Seed transmission of Cowpea mild mottle virus in common beans in Brazil. Autoria: PINHEIRO-LIMA, B.; VIDAL, A. H.; FELIX, G. P.; ALVES-FREITAS. D. M. T.; LACORTE, C. C.; FARIA, J. C.; ABREU, E. F. M.; PINHEIRO, P. V.; MELO, F. L. de; RIBEIRO, S. da G. Conteúdo: Cowpea mild mottle virus (CPMMV) is a carlavirus that is transmitted by whiteflies and can also be spread through seeds in cowpea, soybean, and common bean. Seed transmission of CPMMV has been described in various countries; however, it was only recently reported in Brazil as a seed-transmitted virus in soybean. CPMMV has spread widely in bean-growing areas in recent years. Most Brazilian farmers use harvested grains as seed for reseeding, increasing the risk of seedborne infection. In this study, we examine seed transmission of CPMMV in two bean cultivars using RT-PCR in combination with nucleic acid hybridization. The plants evaluated were obtained from seeds harvested in commercial and experimental fields and from seeds collected in transmission experiments using either mechanical or whitefly inoculation. The observed seed transmission was estimated between 10 to 45% for ‘BRS FC 401 RMD’ and 13 to 22% for ‘Pérola’. In addition, evidence of secondary transmission (23.3%) by Bemisia tabaci MEAM1 was observed. These results suggest that CPMMV could be spread in bean fields by sowing infected seeds and that germinated plants could serve as an inoculum for whitefly transmission. This is the first report of seed transmission of CPMMV in common bean in Brazil. Thu, 01 Jan 2026 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1189040 2026-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1188953 Título: Overexpression of a major latex-like protein from wild Arachis (AdMLP11) confers tolerance to recurrent drought stress. Autoria: SPECK, A.; GOMES, H. T.; SARAIVA, M. A. de P.; GUIMARAES, P. M.; BRASILEIRO, A. C. M. Conteúdo: Recurrent drought episodes, increasingly intensified by climate change, pose a growing threat to global food security by severely limiting crop productivity. Major latex-like proteins (MLPs) play crucial roles in drought tolerance, acting as regulators of stress responses. However, their involvement in adaptation to recurrent drought stress remains poorly understood. In this study, we investigated the transcriptional dynamics of MLP genes during repeated dehydration and rehydration cycles in Arachis duranensis, a tropical wild species highly resilient to drought. In silico expression profiling of 36 A. duranensis MLP genes revealed their broad involvement in recurrent drought responses, with most patterns consistent with the ‘revised-response’ category of dehydration memory genes. qRT-PCR analysis further confirmed the activation of the abscisic acid (ABA) signaling pathway during recurrent drought in A. duranensis. Functional characterization of the candidate memory gene AdMLP11 in transgenic tobacco showed that its overexpression enhances tolerance to moderate and severe recurrent drought, likely through its role as a positive regulator of phytohormone-mediated defense pathways. These findings provide novel insights into the role of MLPs in transcriptional memory and drought adaptation in wild Arachis, highlighting AdMLP11 as a promising target for biotechnological strategies to develop climate-resilient crops. Thu, 01 Jan 2026 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1188953 2026-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1188940 Título: Characterization of the insect assemblage associated with two weed species in a rice-growing area. Autoria: GRECO, M. G. C. E.; ABEIJON, L. M.; WEBER, E. M. D.; SILVA, E.; SPECHT, A.; LUTINSKI, J. A.; AGOSTINETTO, D.; GARCIA, F. R. M. Conteúdo: Rice is essential to global food security, and its crops are challenged by the presence of Aeschynomene Fabaceae (jointvetch), whose control by herbicides generates environmental, health and resistance risks. Thus, this study aims to characterizing the assemblage of phytophagous insects associated with A. denticulata and A. indica during two rice harvests, and cooperates in identifying possible biocontrol agents and understanding the role these plants play as alternative hosts of insects that have agricultural pest status. A total of 300 plants were inspected where phytophagous entomofauna was collected. Faunal analysis was performed, including its abundance, constancy, dominance, frequency, richness, diversity, equitability, rarefaction and principal component analysis (PCA). Acanthoscelides atrocephalus, Morphospecie 6, Ceresa sp., Morphospecie 2 and Euschistus heros were predominant when collecting the two plants. For the first time, 15 species of insects associated with A. denticulata and 16 with A. indica were recorded. Among the insects found, species such as A. atrocephalus present in the seeds and Tyloderma sp. (observed in the roots) showed potential for future research in Integrated Weed Management as bioagents. The observed association between jointvetch and economic interest insects, such as E. heros, suggests that these weeds may favor the maintenance of these populations in the rice-growing area. These results contribute to the development of sustainable strategies in weed management for rice cultivation, and expand knowledge about ecological interactions of these species. Thu, 01 Jan 2026 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1188940 2026-01-01T00:00:00Z