https://www.alice.cnptia.embrapa.br/alice/handle/item/394 Artigo em periódico indexado (CNPAE) Thu, 02 Jul 2026 23:31:27 GMT 2026-07-02T23:31:27Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187917 Título: Agronomic performance and seed quality of canola cultivars grown at high altitudes in the Brazilian Cerrado. Autoria: MELO, S. G. F. de; SILVA, I. J.; SANTANA, R. A.; ZANÚNCIO, J. C.; GUIMARÃES, M. de A.; LAVIOLA, B. G.; EVARISTO, A. B.; NERY, M. C. Conteúdo: Canola (Brassica napus L. var. oleifera) is a major oilseed crop for human consumption and biodiesel production, yet its agronomic performance and seed quality under high-altitude tropical conditions are not well documented. This study evaluated four canola cultivars (Alth B4, Diamond, Hyola 575 CL®, and Nuola 300) grown at an altitude of 1,387 m above sea level in the Brazilian Cerrado, a savanna area in Diamantina, Minas Gerais, Brazil. The experiment followed a completely randomized design with four replications, assessing plant growth, productivity, seed physiological quality, and oil content. The phenological cycle of the cultivars ranged from 140 to 146 days. Productivity was highest for Nuola 300 and Alth B4 (~2,034 kg ha-¹), exceeding the national average, while Diamond and Alth B4 exhibited superior seed vigor and germination performance. Oil content varied from 42.57% to 46.36%, with Alth B4 and Diamond showing the highest values. Principal component analysis highlighted strong associations between agronomic performance and seed quality, distinguishing Diamond, Nuola 300, and Alth B4 as the most adaptable to high-altitude savanna conditions, while Hyola 575 showed lower overall performance. These findings demonstrate that canola can achieve high productivity and seed quality in high-altitude Cerrado regions, identifying cultivars with strong potential for commercial cultivation in these conditions. Thu, 01 Jan 2026 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187917 2026-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187682 Título: Energy–environmental nexus in green hydrogen-assisted conversion of second-crop canola to sustainable aviation fuel: toward low-carbon bioenergy systems. Autoria: LAMAS, G. C.; CARDOSO, A. N.; SABAINI, P. S.; LUZ, S. M.; BORGES, M. dos R. S.; COSTA, T. da S.; GONZALES, T. da S.; MATSUURA, M. I. da S. F.; LAVIOLA, B. G.; RODRIGUES, T. O.; ROUSSET, P.; SILVEIRA, E. A. Conteúdo: Abstract: Aviation fuel remains the main cost and environmental burden in air transport. This study presents a well-to-wake life cycle assessment (LCA) of canola-based Sustainable Aviation Fuel (SAF) under tropical conditions, based on primary data from Brazilian producers. The analysis encompasses agricultural, pre-processing, and conversion stages via the hydroprocessed esters and fatty acids (HEFA) pathway, revealing the potential of second-crop canola for low-carbon aviation. The study integrates process modeling, renewable hydrogen, and land-use efficiency to capture drivers across stages. The ReCiPe method was applied to 1 MJ of biokerosene as the functional unit. Agriculture dominates GHG emissions (34.2 g CO2 eq. MJ−1), driven primarily by fertilizer production and soil N2O emissions, while the HEFA phase contributes 12.8 g CO2 eq. MJ−1. Substituting fossil hydrogen with photovoltaic- and wind-based hydrogen for in HEFA upgrading reduces emissions by 92 to 96.6%, resulting in up to 19.6% lower total life-cycle emissions. Compared to Jet-A1, SAF decreases fossil depletion by 59% and achieves climate benefits; however, it entails higher burdens in selected non-climate impact categories. Freshwater and marine eutrophication reach approximately 0.01 g P eq. MJ−1 and 0.7 g N eq. MJ−1, respectively, while human toxicity is above 1 g 1,4-DB eq. MJ−1, with the agricultural stage accounting for over 90% of these impacts, particularly fertilizer production and use. Land occupation (0.074 m2 yr MJ−1) is optimized through canola soybean rotation, mitigating deforestation risks. The findings demonstrate canola's strategic role in Brazil's decarbonization policies, highlighting the need for improved fertilizer management and renewable hydrogen integration to advance SAF. Thu, 01 Jan 2026 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187682 2026-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187414 Título: Enhancing wheat bran deconstruction with enzyme cocktails from Penicillium spp. and Trichoderma harzianum: the impact of β-glucosidase supplementation. Autoria: HAMANN, P. R. V.; MARTINS, H. S.; COSTA, P. H. C. da; BRITO, D. T. de A.; SILVA, V. R. C. da; TAVARES, Y. F.; QUIRINO, B. F.; NORONHA, E. F. Conteúdo: Abstract: Forests and natural environments are valuable sources of organisms capable of degrading plant cell wall carbohydrates. In the present study, two isolates, Penicillium polonicum and Penicillium chrysogenum, obtained from Brazilian Cerrado soil, along with a previously characterized isolate of Trichoderma harzianum, were evaluated for their ability to produce carbohydrate-active enzymes when grown in the presence of wheat bran. P. chrysogenum exhibited higher production of endoglucanases, xylanases, and pectinases. Biochemical characterization showed that most enzymes were active at pH 5 and within a temperature range of 30–50 °C, with the exception of P. chrysogenum endoglucanases, which displayed optimal activity at 60 °C. Hydrolysis of cellulose and carboxymethyl cellulose by the three enzyme sources demonstrated that supplementation with recombinant β-glucosidase from Clostridium thermocellum (BglA) significantly enhanced reducing sugar release; specifically, when applied to P. chrysogenum, the reducing sugar yield from cellulose hydrolysis increased by 2.53-fold. In the hydrolysis of wheat bran, enzymes from P. chrysogenum and T. harzianum supplemented with BglA resulted in a 1.6- and 3.93-fold increase in D-glucose yield, respectively. The results presented in this study contribute to the development of more robust enzyme cocktails for wheat bran valorization. Thu, 01 Jan 2026 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187414 2026-01-01T00:00:00Z https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187279 Título: A new GH3 B-Glucosidase from Chryseobacterium sp. with applications in cellulosic ethanol production and agri-biotechnological processes. Autoria: BERGMANN, J. C.; LACERDA, V. A. M.; ALENCAR, K. L. C.; FAVARO, L. C. de L.; RODRIGUES, D. de S.; MARINS, L. F.; QUIRINO, B. F. Conteúdo: Abstract: β-Glucosidases catalyze the hydrolysis of β-glycosidic bonds and play key roles in biomass conversion and glycoside processing. We report the identification and characterization of Cr_B1, a GH3 β-glucosidase from Chryseobacterium sp. containing a predicted signal peptide. Cr_B1 hydrolyzed pNPG, cellobiose, salicin, and daidzin, showing optimal activity at pH 5.0−5.5 and 45−55 °C. The enzyme retained over 90% activity after 190 days at 4°C and 25 °C and above 80% activity after 24 h at 50 °C, indicating remarkable long-term and thermal stability. Cr_B1 exhibited high glucose tolerance (IC50: 1.5−1.8 M) and substrate-dependent kinetics. In synergy with Celluclast, it increased glucose release from CMC by 69%, demonstrating its potential to enhance enzymatic saccharification. These properties highlight Cr_B1 as a promising biocatalyst for improving saccharification, enhancing isoflavone bioavailability, and reducing bitterness in food and feed applications. Thu, 01 Jan 2026 00:00:00 GMT https://www.alice.cnptia.embrapa.br/alice/handle/doc/1187279 2026-01-01T00:00:00Z