Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/105490
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dc.contributor.authorMOURA-COSA, P.H.pt_BR
dc.contributor.authorMANTELL, S. H.pt_BR
dc.date.accessioned2011-04-09T12:13:38Z-
dc.date.available2011-04-09T12:13:38Z-
dc.date.created2001-08-22pt_BR
dc.date.issued1993pt_BR
dc.identifier.citationPesquisa Agropecuaria Brasileira, Brasilia, v.28, n.11, p.1297-1306,nov.1993pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/105490pt_BR
dc.descriptionEffect of different temperatures (15, 19, 25 and 29 °C), media type (PDA and Czapek Dox agar) and light on sporulation of a Caribbean pathovar of Colletotrichum gloe-osporioides f.sp. dioscoreae were studied. Czapek Dox agar and temperature of 25 °C were found to be best conditions for supporting growth and sporulation of this organism. Long-term fungal cultures (2 years axenic culture) did not sporulate effectively under the above conditions unless they were first brought into contact with living tissues of Dioscorea. Methods for inoculation of yams with conidiospores of C. gloeosporioides were developed at the plant, mi-croplant and cell levels. In vitro shoot culture clones showed a similar range of disease reactions to the pathovar as field-grown plants. D. alata showed marked sensitivity to the disease while D. esculenta was the most tolerant species followed by D. composita and D. cayenensis. Dioscorea protoplasts responded differently when cultured in the presence of mycelial culture filtrates and Czapek Dox liquid medium. Glasshouse-raised plants developed anthracnose symptoms only after wounding.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectColletotrichum gloesporioides fpt_BR
dc.subjectsppt_BR
dc.subjectdioscoreaept_BR
dc.subjectCzapek Dox agapt_BR
dc.subjectmicroplantpt_BR
dc.titleA Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2011-04-10T11:11:11Zpt_BR
dc.subject.nalthesaurusclonespt_BR
riaa.ainfo.id105490pt_BR
riaa.ainfo.lastupdate2004-09-27pt_BR
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