Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1083330
Research center of Embrapa/Collection: Embrapa Café - Artigo em periódico indexado (ALICE)
Date Issued: 2017
Type of Material: Artigo em periódico indexado (ALICE)
Authors: KOBAYASHI, A. K.
VIEIRA, L. G. E.
BESPALHOK FILHO, J. C.
LEITE, R. P.
PEREIRA, L. F. P.
MOLINARI, H. B. C.
MARQUES, V. V.
Additional Information: Adilson K. Kobayashi; Luiz Gonzaga E. Vieira; João Carlos Bespalhok Filho; Rui Pereira Leite; LUIZ FILIPE PROTASIO PEREIRA, SAPC; Hugo Bruno C. Molinari; Viviani V. Marques.
Title: Enhanced resistance to citrus canker in transgenic sweet orange expressing the sarcotoxin IA gene.
Publisher: European Journal of Plant Pathology, v. 149, n. 4, p. 865–873, Dezembro 2017.
Language: en
Keywords: Citrus canker
Mature tissue transformation
Bacterial disease resistance
Description: Citrus canker, caused by the bacterial pathogen Xanthomonas citri subp. Citri (Xcc), is a serious disease reported in most citrus-producing areas around the world. Although different levels of field resistance to citrus canker have been reported in sweet oranges, they are usually not sufficient to provide adequate control of the disease. Ectopic over-expression of antibacterial genes is one of the potential strategies to increase plant resistance to bacterial diseases. Previous in vitro results showed that sarcotoxin IA, an antimicrobial peptide isolated from the flesh fly (Sarcophaga peregrina), can be efficient to control different plant pathogenic bacteria, including Xcc. Transgenic BPera^ sweet orange (Citrus sinensis [L.] Osbeck) plants constitutively expressing the sarcotoxin IA peptide fused to the PR1a signal peptide from Nicotiana tabacum for secretion in the intercellular space were obtained by Agrobacteriummediated transformation using thin sections of mature explants. Citrus canker resistance evaluation in leaves of transgenic and non-transgenic plants was performed through inoculations with Xcc by infiltration and spraying. The Xcc population was up to 2 log unit lower in leaves of the transgenic plants compared to those of non-transgenic controls. Incidence of canker lesions was significantly higher in non-transformed controls (>10 lesions/cm2) than in the transgenic plants (<5 lesions/ cm2) after injection infiltration or spraying with Xcc inoculum. Accumulation of sarcotoxin IA peptide in sweet orange tissue did not cause any deleterious effects on the growth and development of the transgenic plants, indicating this approach is suitable to provide resistance to citrus canker.
NAL Thesaurus: Antimicrobial peptides
Agrobacterium radiobacter
Data Created: 2017-12-21
Appears in Collections:Artigo em periódico indexado (SAPC)

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