Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1046809
Unidade da Embrapa/Coleção:: Embrapa Soja - Artigo em periódico indexado (ALICE)
Data do documento: 13-Jun-2016
Tipo do Material: Artigo em periódico indexado (ALICE)
Autoria: ARDISSON-ARAÚJO, D. M. P.
LIMA, R. N.
MELO, F. L.
CLEM, R. J.
HUANG, N.
BÁO, S. N.
SOSA-GÓMEZ, D. R.
RIBEIRO, B. M.
Informaçães Adicionais: DANIEL M. P. ARDISSON-ARAÚJO, UNB; RAYANE NUNES LIMA, UNB; FERNANDO L. MELO, UNB; ROLLIE J. CLEM, KANSAS STATE UNIVERSITY; NING HUANG, Kansas State University; SÔNIA NAIR BÁO, UNB; DANIEL RICARDO SOSA GOMEZ, CNPSO; BERGMANN M. RIBEIRO, UNB.
Título: Genome sequence of Perigonia lusca single nucleopolyhedrovirus: insights into the evolution of a nucleotide metabolism enzyme in the family Baculoviridae.
Edição: 2016
Fonte/Imprenta: Scientific Reports, v. 6, n. 24612, jun. 2016.
Idioma: en
Conteúdo: The genome of a novel group II alphabaculovirus, Perigonia lusca single nucleopolyhedrovirus (PeluSNPV), was sequenced and shown to contain 132,831bp with 145 putative ORFs (open reading frames) of at least 50 amino acids. An interesting feature of this novel genome was the presence of a putative nucleotide metabolism enzyme-encoding gene (pelu112). The pelu112 gene was predicted to encode a fusion of thymidylate kinase (tmk) and dUTP diphosphatase (dut). Phylogenetic analysis indicated that baculoviruses have independently acquired tmk and dut several times during their evolution. Two homologs of the tmk-dut fusion gene were separately introduced into the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) genome, which lacks tmk and dut. The recombinant baculoviruses produced viral DNA, virus progeny, and some viral proteins earlier during in vitro infection and the yields of viral occlusion bodies were increased 2.5-fold when compared to the parental virus. Interestingly, both enzymes appear to retain their active sites, based on separate modeling using previously solved crystal structures. We suggest that the retention of these tmk-dut fusion genes by certain baculoviruses could be related to accelerating virus replication and to protecting the virus genome from deleterious mutation.
Thesagro: Baculovirus
Virus
Genoma
NAL Thesaurus: Baculoviridae
Genome assembly
Ano de Publicação: 2016
ISSN: 2045-2322
Aparece nas coleções:Artigo em periódico indexado (CNPSO)

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