Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1060930
Research center of Embrapa/Collection: Embrapa Meio Ambiente - Artigo em periódico indexado (ALICE)
Date Issued: 2017
Type of Material: Artigo em periódico indexado (ALICE)
Authors: BAPTISTA, R. C.
FERNANDES, M. A. M.
GILAVERTE, S.
QUEIROZ, S. C. do N. de
ASSALIN, M. R.
FERRACINI, V. L.
MONTEIRO, A. L. G.
REYES, F. G. R.
Additional Information: RAFAELA DE CARVALHO BAPTISTA, FEA-UNICAMP; MARIA ANGELA MACHADO FERNANDES, LAPOC-UFPR; SUSANA GILAVERTE, LAPOC-UFPR; SONIA CLAUDIA DO N DE QUEIROZ, CNPMA; MARCIA REGINA ASSALIN, CNPMA; VERA LUCIA FERRACINI, CNPMA; ALDA LUCIA GOMES MONTEIRO, LAPOC-UFPR; FELIX GUILLERMO REYES REYES, FEA-UNICAMP.
Title: Determination of moxidectin in serum by liquid chromatography-tandem mass spectrometry and its application in pharmacokinetic study in lambs.
Publisher: Journal of the Brazilian Chemical Society, Campinas, v. 28, n. 2, p. 250-256, 2017.
Language: en
Keywords: Serum lamb
LC-MS/MS
Soro sanguíneo.
Description: Abstract: The widespread use of moxidectin (MOX), a parasiticide used in the sheep breeding, has induced the parasite resistance in Brazilian farms. As a consequence, the farmers often increase the dose and frequency of drug utilization, and disregards safety of meat or milk. In order to establish adequate therapeutic treatment it is necessary to know the pharmacokinetics of the drug in the animal's body. Thus, high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) method was developed for the determination of MOX in serum lamb. Serum samples were treated with acetonitrile to precipitate proteins. A clean up by dispersive extraction in solid phase (SPE-d), using primary/secondary amine (PSA) and C18 sorbents, followed by freezing was performed. Method validation presented precision (coefficient of variation) and accuracy (recovery%) between 1.7-6.7 and 80.0-107.3%, respectively. The limit of quantification (LOQ) of the method was 2.0 ng mL-1 and a linear response was obtained over a range of 2.0 to 100 ng mL-1. This method was successfully applied to the determination of MOX in serum from suffolk lamb to evaluate the pharmacokinetic profile.
Thesagro: Cordeiro
Análise de laboratório
Anti-helmíntico
Cromatografia
Espectrometria.
NAL Thesaurus: Blood serum
Lambs
Moxidectin
Anthelmintics
Pharmacokinetics
Veterinary drugs
Tandem mass spectrometry
Liquid chromatography
Data Created: 2017-01-17
Appears in Collections:Artigo em periódico indexado (CNPMA)

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