Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1131989
Research center of Embrapa/Collection: Embrapa Arroz e Feijão - Capítulo em livro científico (ALICE)
Date Issued: 2021
Type of Material: Capítulo em livro científico (ALICE)
Authors: PRADO, G. S.
PINHEIRO, T. T.
FARIA, J. C. de
VIANELLO, R. P.
Additional Information: GUILHERME SOUZA PRADO, especialista visitante CNPAF; THAÍSA TESSUTTI PINHEIRO, especialista visitante CNPAF; JOSIAS CORREA DE FARIA, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF.
Title: Genome editing via non-homologous end-joining (NHEJ) and ribonucleoproteins (RNP).
Publisher: In: MOLINARI, H. B. C.; VIEIRA, L. R.; SILVA, N. V. e; PRADO, G. S.; LOPES FILHO, J. F. (Ed.). CRISPR technology in plant genome editing: biotechnology applied to agriculture. Brasília, DF : Embrapa, 2021.
Pages: p. 47-88.
Language: Ingles
Keywords: CRISPR
CRISPRevolution
Non-homologous
Description: We will focus here on the use of the CRISPR/Cas system to induce mutations via NHEJ. The NHEJ mechanism acts in most of the cell cycle and is not based on homology to repair DNA, causing mutations such as insertions or deletions (indels) or even substitutions. In this way, the expression of the genes in the place where the repair occurred by NHEJ can, in general, be interrupted, and the encoded proteins may not be functional. To clarify the process of genome editing by CRISPR, this chapter aims to address the details of the NHEJ mechanism, as well as the use of ribonucleoprotein systems (RNPs) to promote editing, in addition to providing a practical procedure for the design of experiments.
Thesagro: Biotecnologia
DNA
RNA
Engenharia Genética
Genoma
NAL Thesaurus: Biotechnology
Genetic engineering
Nucleases
Plant genetics
Ribonucleoproteins
RNA editing
Data Created: 2021-05-24
ISBN: 978-65-87380-10-0
Appears in Collections:Capítulo em livro científico (CNPAF)

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