Title:	Genome editing via non-homologous end-joining (NHEJ) and ribonucleoproteins (RNP).
Authors:	PRADO, G. S. PINHEIRO, T. T. FARIA, J. C. de VIANELLO, R. P.
Affiliation:	GUILHERME SOUZA PRADO, especialista visitante CNPAF; THAÍSA TESSUTTI PINHEIRO, especialista visitante CNPAF; JOSIAS CORREA DE FARIA, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF.
Date Issued:	2021
Citation:	In: MOLINARI, H. B. C.; VIEIRA, L. R.; SILVA, N. V. e; PRADO, G. S.; LOPES FILHO, J. F. (Ed.). CRISPR technology in plant genome editing: biotechnology applied to agriculture. Brasília, DF : Embrapa, 2021.
Pages:	p. 47-88.
Description:	We will focus here on the use of the CRISPR/Cas system to induce mutations via NHEJ. The NHEJ mechanism acts in most of the cell cycle and is not based on homology to repair DNA, causing mutations such as insertions or deletions (indels) or even substitutions. In this way, the expression of the genes in the place where the repair occurred by NHEJ can, in general, be interrupted, and the encoded proteins may not be functional. To clarify the process of genome editing by CRISPR, this chapter aims to address the details of the NHEJ mechanism, as well as the use of ribonucleoprotein systems (RNPs) to promote editing, in addition to providing a practical procedure for the design of experiments.
Thesagro:	Biotecnologia DNA RNA Engenharia Genética Genoma
NAL Thesaurus:	Biotechnology Genetic engineering Nucleases Plant genetics Ribonucleoproteins RNA editing
Keywords:	CRISPR CRISPRevolution Non-homologous
ISBN:	978-65-87380-10-0
Type of Material:	Parte de livro
Access:	openAccess
Appears in Collections:	Capítulo em livro científico (CNPAF)