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|Title:||Marker-assisted pyramiding of multiple disease resistance genes in coffee genotypes (coffea arabica).|
|Authors:||ALMEIDA, D. P. de|
CAIXETA, E. T.
MOREIRA, K. F.
OLIVEIRA, A. C. B. de
FREITAS, K. N. P. de
PEREIRA, A. A.
ROSADO, R. D. S.
CRUZ, C. D.
|Affiliation:||DÊNIA PIRES DE ALMEIDA, UFV; EVELINE TEIXEIRA CAIXETA MOURA, CNPCa; KAROLINY FERREIRA MOREIRA, UFV; ANTONIO CARLOS BAIAO DE OLIVEIRA, CNPCa; KÁTIA NOGUEIRA PESTANA DE FREITAS, UFV; ANTÔNIO ALVES PEREIRA, EPAMIG; RENATO DOMICIANO SILVA ROSADO, UFV; LAÉRCIO ZAMBOLIM, UFV; COSME DAMIÃO CRUZ, UFV.|
|Citation:||Agronomy, v. 11, n. 9, 1763, 2021.|
|Description:||The use of resistant cultivars is the most effective strategy for controlling coffee leaf rust caused by the fungus Hemileia vastatrix. To assist the development of such cultivars, amplified fragment-length polymorphism (AFLP) markers linked to two loci of coffee resistance to races I and II as well as pathotype 001 of H. vastatrix were converted to sequence-characterized amplified region (SCAR) and cleaved amplified polymorphic site (CAPS) markers. In total, 2 SCAR markers and 1 CAPS marker were validated in resistant and susceptible parents as well as in 247 individuals from the F2 population. The efficiency of these markers for marker-assisted selection (MAS) was evaluated in F2:3 and backcross (BCrs2) populations genotyped with the developed markers and phenotyped with race II of H. vastatrix. The markers showed 90% efficiency in MAS. Therefore, the developed markers, together with molecular markers associated with other rust resistance genes, were used for F3:4 and BCrs3 coffee selection. The selected plants were analyzed using two markers associated with coffee berry disease (CBD) resistance, aiming for preventive breeding. MAS of F3:4 and BCrs3 individuals with all resistance loci was feasible. Our phenotypic and genotypic approaches are useful for the development of coffee genotypes with multiple genes conferring resistance to coffee leaf rust and CBD.|
|NAL Thesaurus:||Plant breeding|
|Type of Material:||Artigo de periódico|
|Appears in Collections:||Artigo em periódico indexado (SAPC)|
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