Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/576277
Title: Karyoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle.
Authors: MEO, S. C.
FERREIRA, C. R.
PERECIN, F.
SARAIVA, N. Z.
TETZNER, T. A. D.
YAMAZAKI, W.
LEA, C. L. V.
MEIRELLES, F. V.
GARCIA, J. M.
Affiliation: SIMONE CRISTINA MEO NICIURA, CPPSE; CHRISTINA RAMIRES FERREIRA, FZEA – USP/PIRASSUNUNGA; FELIPE PERECIN, FZEA – USP/PIRASSUNUNGA; NAIARA ZOCCAL SARAIVA, FCAV – UNESP/JABOTICABAL; TATIANE ALMEIDA DRUMMOND TETZNER, FCAV – UNESP/JABOTICABAL; WALT YAMAZAKI, FCAV–UNESP/JABOTICABAL; CLÁUDIA LIMA VERDE LEA, FZEA–USP/PIRASSUNUNGA; FLÁVIO VIEIRA MEIRELLES, FZEA–USP/PIRASSUNUNGA; JOAQUIM MANSANO GARCIA, FCAV–UNESP/JABOTICABAL.
Date Issued: 2009
Citation: Animal Reproduction Science, v. 116, n. 3-4, p. 381?385, 2009
Description: Ooplasmic factors drive nuclear organization after fertilization and are also important for re-programming in nuclear transfer procedures, in which artificial activation is essential for reconstructed embryos to progress in development. The present research evaluated the effect of pronuclear transfer (PT) between zygotes parthenogenetically activated with ionomycin followed by strontium (S) or 6-DMAP (D) on early embryonic development. PT was performed in the same zygote to obtain embryos in control groups (S-PT and D-PT) and between zygotes activated with S and D to achieve embryos with differentially activated cytoplasm (C) and nucleus (N) (SCDN and DCSN). PT procedure did not affect cleavage and blastocyst rates, respectively, in PT control groups compared to non-manipulated control (S-PT: 73.6% and 7.3% compared with S-Control: 77.9% and 7.8%; and D-PT: 73.3% and 31.7% compared with D-Control: 83.1% and 41.5%). Cleavage, eight-cell, and blastocyst rates, respectively, were similar between SCDN (76.5%, 36.4%, and 6.8%) and DCSN (69.5%, 25.0%, and 4.9%) embryos. Developmental rates in SCDN were similar to S-PT, but inferior to D-PT.
NAL Thesaurus: beef cattle.
Keywords: Artificial activation
Bovine
Embryonic development
Pronuclear transfer.
Language: pt_BR
Type of Material: Separatas
Access: openAccess
Appears in Collections:Artigo em periódico indexado (CPPSE)

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