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Unidade da Embrapa/Coleção:: Embrapa Pecuária Sudeste - Artigo em periódico indexado (ALICE)
Data do documento: 26-Nov-2009
Tipo do Material: Artigo em periódico indexado (ALICE)
Autoria: MEO, S. C.
FERREIRA, C. R.
PERECIN, F.
SARAIVA, N. Z.
TETZNER, T. A. D.
YAMAZAKI, W.
LEA, C. L. V.
MEIRELLES, F. V.
GARCIA, J. M.
Informaçães Adicionais: SIMONE CRISTINA MEO NICIURA, CPPSE; CHRISTINA RAMIRES FERREIRA, FZEA – USP/PIRASSUNUNGA; FELIPE PERECIN, FZEA – USP/PIRASSUNUNGA; NAIARA ZOCCAL SARAIVA, FCAV – UNESP/JABOTICABAL; TATIANE ALMEIDA DRUMMOND TETZNER, FCAV – UNESP/JABOTICABAL; WALT YAMAZAKI, FCAV–UNESP/JABOTICABAL; CLÁUDIA LIMA VERDE LEA, FZEA–USP/PIRASSUNUNGA; FLÁVIO VIEIRA MEIRELLES, FZEA–USP/PIRASSUNUNGA; JOAQUIM MANSANO GARCIA, FCAV–UNESP/JABOTICABAL.
Título: Karyoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle.
Edição: 2009
Fonte/Imprenta: Animal Reproduction Science, v. 116, n. 3-4, p. 381?385, 2009
Idioma: pt_BR
Palavras-chave: Artificial activation
Bovine
Embryonic development
Pronuclear transfer.
Conteúdo: Ooplasmic factors drive nuclear organization after fertilization and are also important for re-programming in nuclear transfer procedures, in which artificial activation is essential for reconstructed embryos to progress in development. The present research evaluated the effect of pronuclear transfer (PT) between zygotes parthenogenetically activated with ionomycin followed by strontium (S) or 6-DMAP (D) on early embryonic development. PT was performed in the same zygote to obtain embryos in control groups (S-PT and D-PT) and between zygotes activated with S and D to achieve embryos with differentially activated cytoplasm (C) and nucleus (N) (SCDN and DCSN). PT procedure did not affect cleavage and blastocyst rates, respectively, in PT control groups compared to non-manipulated control (S-PT: 73.6% and 7.3% compared with S-Control: 77.9% and 7.8%; and D-PT: 73.3% and 31.7% compared with D-Control: 83.1% and 41.5%). Cleavage, eight-cell, and blastocyst rates, respectively, were similar between SCDN (76.5%, 36.4%, and 6.8%) and DCSN (69.5%, 25.0%, and 4.9%) embryos. Developmental rates in SCDN were similar to S-PT, but inferior to D-PT.
NAL Thesaurus: beef cattle.
Ano de Publicação: 2009
Aparece nas coleções:Artigo em periódico indexado (CPPSE)

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