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dc.contributor.authorMARTINS, K. B.pt_BR
dc.contributor.authorFACCIOLI-MARTINS, P. Y.pt_BR
dc.contributor.authorRIBOLI, D. F. M.pt_BR
dc.contributor.authorPEREIRA, V. C.pt_BR
dc.contributor.authorFERNANDES, S.pt_BR
dc.contributor.authorOLIVEIRA, A. A.pt_BR
dc.contributor.authorDANTAS, A.pt_BR
dc.contributor.authorZAFALON, L. F.pt_BR
dc.contributor.authorCUNHA, M. de L. R. de S. dapt_BR
dc.date.accessioned2015-11-26T11:11:11Zpt_BR
dc.date.available2015-11-26T11:11:11Zpt_BR
dc.date.created2015-11-26pt_BR
dc.date.issued2015pt_BR
dc.identifier.citationBrazilian Journal of Microbiology, v. 46, n. 2, p. 535-543, apr./jun. 2015.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1029706pt_BR
dc.descriptionThe objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S. aureus was investigated by pulsed-field gel electrophoresis. A total of 473 milk samples were collected from 242 animals on three farms and 20 S. aureus strains were isolated and none carried the mecA gene. The two sec gene-positive isolates and the isolates carrying the tst and luk-PV genes were positive by RT-PCR. Staphylococcus aureus isolated from the three flocks studied showed high susceptibility to the drugs tested and none was biofilm producer, indicating that biofilm formation was not a virulence factor causing infection by these strains. The typing of 17 S. aureus isolates revealed the presence of a common clone on the three farms studied, and the presence and expression of the sec and tst genes in one strain of this clone suggest the possible acquisition of virulence genes by this clone, a fact that is important for animal health and food hygiene.eng
dc.language.isoengpt_BR
dc.rightsopenAccesspt_BR
dc.subjectVirulence factorspt_BR
dc.titleClonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2016-03-02T11:11:11Zpt_BR
dc.subject.thesagroStaphylococcus Aureuspt_BR
dc.subject.nalthesaurusantibiotic resistancept_BR
dc.subject.nalthesaurusmastitispt_BR
dc.subject.nalthesaurussheeppt_BR
riaa.ainfo.id1029706pt_BR
riaa.ainfo.lastupdate2016-03-02pt_BR
dc.identifier.doi10.1590/S1517-838246220131164pt_BR
dc.contributor.institutionKATHERYNE BENINI MARTINS, Universidade Estadual Paulistapt_BR
dc.contributor.institutionPATRICIA YOSHIDA FACCIOLI-MARTINS, Universidade Estadual Paulistaeng
dc.contributor.institutionDANILO FLÁVIO MORAES RIBOLI, Universidade Estadual Paulistaeng
dc.contributor.institutionVALÉRIA CATANELI PEREIRA, Universidade Estadual Paulistaeng
dc.contributor.institutionSIMONE FERNANDES, Universidade Estadual Paulistaeng
dc.contributor.institutionALINE A. OLIVEIRA, Universidade Estadual Paulistaeng
dc.contributor.institutionARIANE DANTAS, Universidade Estadual Paulistaeng
dc.contributor.institutionLUIZ FRANCISCO ZAFALON, CPPSEeng
dc.contributor.institutionMARIA DE LOURDES RIBEIRO DE SOUZA DA CUNHA, Universidade Estadual Paulista.eng
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