Title:	Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk.
Authors:	MARTINS, K. B. FACCIOLI-MARTINS, P. Y. RIBOLI, D. F. M. PEREIRA, V. C. FERNANDES, S. OLIVEIRA, A. A. DANTAS, A. ZAFALON, L. F. CUNHA, M. de L. R. de S. da
Affiliation:	KATHERYNE BENINI MARTINS, Universidade Estadual Paulista PATRICIA YOSHIDA FACCIOLI-MARTINS, Universidade Estadual Paulista DANILO FLÁVIO MORAES RIBOLI, Universidade Estadual Paulista VALÉRIA CATANELI PEREIRA, Universidade Estadual Paulista SIMONE FERNANDES, Universidade Estadual Paulista ALINE A. OLIVEIRA, Universidade Estadual Paulista ARIANE DANTAS, Universidade Estadual Paulista LUIZ FRANCISCO ZAFALON, CPPSE MARIA DE LOURDES RIBEIRO DE SOUZA DA CUNHA, Universidade Estadual Paulista.
Date Issued:	2015
Citation:	Brazilian Journal of Microbiology, v. 46, n. 2, p. 535-543, apr./jun. 2015.
Description:	The objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S. aureus was investigated by pulsed-field gel electrophoresis. A total of 473 milk samples were collected from 242 animals on three farms and 20 S. aureus strains were isolated and none carried the mecA gene. The two sec gene-positive isolates and the isolates carrying the tst and luk-PV genes were positive by RT-PCR. Staphylococcus aureus isolated from the three flocks studied showed high susceptibility to the drugs tested and none was biofilm producer, indicating that biofilm formation was not a virulence factor causing infection by these strains. The typing of 17 S. aureus isolates revealed the presence of a common clone on the three farms studied, and the presence and expression of the sec and tst genes in one strain of this clone suggest the possible acquisition of virulence genes by this clone, a fact that is important for animal health and food hygiene.
Thesagro:	Staphylococcus Aureus
NAL Thesaurus:	antibiotic resistance mastitis sheep
Keywords:	Virulence factors
DOI:	10.1590/S1517-838246220131164
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPPSE)