Title:	Polymorphisms in the DGAT1 gene in buffaloes (Bubalus bubalis) in the Amazon.
Authors:	SILVA, C. S. SILVA FILHO, E. MATOS, A. S. SCHIERHOLT, A. S. COSTA, M. R. MARQUES, L. C. COSTA, J. S. SALES, R. L. FIGUEIRÓ, M. R. MARQUES, J. R. F.
Affiliation:	C. S. Silva, UFPA; E. Silva Filho, UFRA; A. S. Matos, UFPA; A. S. Schierholt, UFRA; MARIA ROSA TRAVASSOS DA ROSA COSTA, CPATU; L. C. Marques, UNAMA; J. S. Costa, UFPA; RODRIGO LIMA SALES, CPATU; MARIVALDO RODRIGUES FIGUEIRO, CPATU; JOSE RIBAMAR FELIPE MARQUES, CPATU.
Date Issued:	2016
Citation:	Genetics and Molecular Research, v. 15, n. 3, 2016.
Description:	Water buffaloes (Bubalus bubalis) are quite well adapted to climatic conditions in the Amazon, and in this biome, they are noted for the considerable amount of meat and milk they produce and how hard they are able to work. Because of a lack of research dedicated to improving the rearing of buffaloes in the Amazon, the objective of this study was to genetically characterize the Murrah and Mediterranean breeds, as well as a mixed-breed population, based on polymorphisms in the diacylglycerol O-acyltransferase 1 gene (DGAT1), and associate the genotypes with milk production. By using the polymerase chain reaction-single-strand conformation polymorphism technique, the alleles A (0.79), B (0.20), and D (0.01) were found in the Murrah breed. In the Mediterranean and mixed-breed buffaloes, we found alleles A (0.69) and (0.77) and B (0.31) and (0.23), respectively. The Murrah breed had the genotypes AA (0.63), AB (0.29), BB (0.05), and AD (0.03), and the Mediterranean and mixed-breed buffaloes had the genotypes AA (0.44) and (0.61), AB (0.50) and (0.31), and BB (0.06) and (0.08), respectively. For the Murrah, Mediterranean, and mixedbreed buffaloes, respectively, the expected heterozygosity values were 0.34, 0.43, and 0.35, the inbreeding coefficients were 0.78, -0.15, and 0.17, and the Hardy-Weinberg probabilities were 0.70, 0.67, and 0.52. The genotypes evaluated did not have an effect on milk production; however, the single nucleotide polymorphisms can be used in studies on genetic variability.
Thesagro:	Búfalo Genótipo Leite
Keywords:	Bubalino
DOI:	http://dx.doi.org/10.4238/gmr.15038720
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPATU)