Title:	Genome sequencing and transcript analysis of Hemileia vastatrix reveal expression dynamics of candidate effectors dependent on host compatibility.
Authors:	PORTO, B. N. CAIXETA, E. T. MATHIONI, S. M. VIDIGAL, P. M. P. ZAMBOLIM, L. ZAMBOLIM, E. M. DONOFRIO, N. POLSON, S. W. MAIA, T. A. CHEN, C. ADETUNJI, M. KINGHAM, B. DALIO, R. J. D. RESENDE, M. L. V. de
Affiliation:	Brenda Neves Porto, Universidade Federal de Lavras - UFLA. Progama de Pós-graduação em Biotecnologia Vegetal EVELINE TEIXEIRA CAIXETA MOURA, CNPCa Sandra Marisa Mathioni, Universidade Federal de Lavras - UFLA/Departamento de Fitopatologia Pedro Marcus Pereira Vidigal, Universidade Federal de Viçosa - UFV/Núcleo de Análises de Biomoléculas Laércio Zambolim, Universidade Federal de Viçosa - UFV/Departamento de Fitopatologia Eunize Maciel Zambolim, Universidade Federal de Viçosa - UFV/Departamento de Fitopatologia Nicole Donofrio, University of Delaware/Department of Plant and Soil Sciences Shawn W. Polson, Delaware Biotechnology Institute/Center for Bioinformatics and Computational Biology Thiago Andrade Maia, Universidade Federal de Viçosa - UFV/Departamento de Fitopatologia Chuming Chen, Delaware Biotechnology Institute/Center for Bioinformatics and Computational Biology Modupe Adetunji, Delaware Biotechnology Institute/Center for Bioinformatics and Computational Biology Brewster Kingham, University of Delaware, Newark/Delaware Biotechnology Institute/Sequencing and Genotyping Center Ronaldo José Durigan Dalio, Instituto Agronoˆmico de Campinas - IAC/Centro de Citricultura “Sylvio Moreira” Mário Lúcio Vilela de Resende, Departamento de Fitopatologia, Universidade Federal de Lavras.
Date Issued:	2019
Citation:	PLOSONE, April 18, 2019.
Description:	Coffee leaf rust caused by the fungus Hemileia vastatrix is one of the most important leaf diseases of coffee plantations worldwide. Current knowledge of the H. vastatrix genome is limited and only a small fraction of the total fungal secretome has been identified. In order to obtain a more comprehensive understanding of its secretome, we aimed to sequence and assemble the entire H. vastatrix genome using two next-generation sequencing platforms and a hybrid assembly strategy. This resulted in a 547 Mb genome of H. vastatrix race XXXIII (Hv33), with 13,364 predicted genes that encode 13,034 putative proteins with transcriptomic support. Based on this proteome, 615 proteins contain putative secretion peptides, and lack transmembrane domains. From this putative secretome, 111 proteins were identified as candidate effectors (EHv33) unique to H. vastatrix, and a subset consisting of 17 EHv33 genes was selected for a temporal gene expression analysis during infection. Five genes were significantly induced early during an incompatible interaction, indicating their potential role as pre-haustorial effectors possibly recognized by the resistant coffee genotype. Another nine genes were significantly induced after haustorium formation in the compatible interaction. Overall, we suggest that this fungus is able to selectively mount its survival strategy with effectors that depend on the host genotype involved in the infection process.
Thesagro:	Hemileia Vastatrix Fungo Ferrugem
NAL Thesaurus:	Genome Leaf rust Infection
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (SAPC)