Title:	Development of an enzyme-coated microcantilever-based biosensor for specific detection of short-chain alcohols.
Authors:	MARGARIDO, A. MANZINE, L. R. ARAUJO-MOREIRA, F. M. GONÇALVES, R. V. HERRMANN JUNIOR, P. S. de P.
Affiliation:	PAULO SERGIO DE P HERRMANN JUNIOR, CNPDIA.
Date Issued:	2021
Citation:	Engineering Proceedings, v. 6, n. 75 2021.
Pages:	1 - 5
Description:	This paper describes the development of a biosensor designed for the enzymatic detection of short-chain alcohols. The biorecognition element, alcohol dehydrogenase, was immobilized on selfassembled monolayers deposited on top of silicon nitride microcantilevers. The self-assembly process was performed by surface activation using 3-aminopropyltriethoxysilane, followed by glutaraldehyde and biomolecule binding. X-ray photoelectron spectroscopy and atomic force microscopy were used. The biosensor showed a lower response time and sensibility from 0.03 to 1.2 mL/L. Its selectivity was analyzed through exposure to pure and mixed volatile solvents. Sensor sensibility was higher in the presence of short-chain alcohols and practically null involving other polar or nonpolar solvents.
Keywords:	Biosensor Microcantilever Biorecognition element
ISSN:	2359-1757
DOI:	https://doi.org/10.3390/I3S2021Dresden-10175
Notes:	Presented at the 8th International Symposium on Sensor Science.
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPDIA)