Title:	CD4 bovine gene: differential polymorphisms among cattle breeds and a new tool for rapid identification.
Authors:	OKINO, C. H. MALAGO JUNIOR, W. MARCONDES, C. R. GIGLIOTI, R. MONTASSIER, H. J. OLIVEIRA, H. N. DE OLIVEIRA, M. C. de S.
Affiliation:	CINTIA HIROMI OKINO, CPPSE; WILSON MALAGO JUNIOR, CPPSE; CINTIA RIGHETTI MARCONDES, CPPSE; RODRIGO GIGLIOTI, Centro de Pesquisa e Desenvolvimento de Genética e Biotecnologia, Instituto de Zootecnia (IZ), Nova Odessa; HÉLIO JOSÉ MONTASSIER, UNESP; HENRIQUE NUNES DE OLIVEIRA, UNESP; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE.
Date Issued:	2022
Citation:	Veterinary Immunology and Immunopathology, v. 251, sep. 2022, 110462.
Pages:	18 p.
Description:	Two mutations in the CD4 bovine gene (G>T/Q306H; A>C/K310N) were identified as causative for altered staining with anti-CD4 mAb #CC8. We developed a HRM qPCR for genotyping these mutations and compare with immunophenotyping in different cattle breeds. The assay distinguished five genotypes, B (homozygous, G/A) and C (heterozygous, G/A and T/C), found in taurine, A (homozygous, G/C) and D (heterozygous, T/C and G/C), found in zebu. The E genotype (homozygous, T/C) was not observed in tested animals. As expected, B and C presented high/very high and intermediate CD4 staining, respectively. The lack/low CD4 staining was mainly related to the A, while the intermediate staining was mainly related to D genotype. The developed HRM qPCR assay accurately identified the altered phenotypes associated with CC8 staining in taurine. However, the assay cannot be applicable in zebu or hybrid breeds, probably due to additional mutations in the CD4 gene from zebu descendant animals.
NAL Thesaurus:	Genotype
Keywords:	QPCR High Resolution Melt Immunophenotyping T helper cells
DOI:	ttps://doi.org/10.1016/j.vetimm.2022.110462
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPPSE)