Title:	Multiplex PCR assay for identification of Corynebacterium pseudotuberculosis from pure cultures and for rapid detection of this pathogen in clinical samples.
Authors:	PACHECO, L. G. C. PENA, R. R. CASTRO, T. L. P. DORELLA, F. A. BAHIA, R. C. CARMINATI, R. FROTA, M. N. L. OLIVEIRA, S. C. MEYER, R. ALVES, F. S. F. MIYOSHI, A. AZEVEDO, V.
Affiliation:	Luis G. C. Pacheco, Instituto de Ciências Biológicas UFMG; Roberta R. Pena, Instituto de Ciências Biológicas UFMG; Thiago L. P. Castro Instituto de Ciências Biológicas UFMG; Fernanda A. Dorella Instituto de Ciências Biológicas UFMG; Robson C. Bahia, UFBA-Departamento de Bio-Interação; Renato Carminati, UFBA-Departamento de Bio-Interação; Maurício N. L. Frota, UVA - Universidade Estadual Vale do Acaraú; Sérgio C. Oliveira, Instituto de Ciências Biológicas UFMG; Roberto Meyer, UFBA-Departamento de Bio-Interação; FRANCISCO SELMO FERNANDES ALVES, CNPC; Anderson Miyoshi, Instituto de Ciências Biológicas UFMG; Vasco Azevedo, Instituto de Ciências Biológicas UFMG.
Date Issued:	2007
Citation:	Journal of Medical Microbiology, v. 56, n. 4, p. 480-486, Apr. 2007.
Description:	Abstract: Corynebacterium pseudotuberculosis is the aetiological agent of caseous lymphadenitis (CLA), a debilitating disease of sheep and goats. Accurate diagnosis of CLA primarily relies on microbiological examination, followed by biochemical identification of isolates. In an effort to facilitate C. pseudotuberculosis detection, a multiplex PCR (mPCR) assay was developed targeting three genes of this bacterium: the 16S rRNA gene, rpoB and pld. This method allowed efficient identification of 40 isolates of this bacterium that had been identified previously by biochemical testing. Analysis of taxonomically related species did not generate the C. pseudotuberculosis mPCR amplification profile, thereby demonstrating the assay's specificity. As little as 1 pg of C. pseudotuberculosis genomic DNA was detected by this mPCR assay, demonstrating the sensitivity of the method. The detection limit in clinical samples was estimated to be 103 c.f.u. C. pseudotuberculosis could be detected directly in pus samples from infected sheep and goats (n=56) with a high diagnostic sensitivity (94.6 %). The developed assay significantly improves rapid C. pseudotuberculosis detection and could supersede bacteriological culture for microbiological and epidemiological diagnosis of CLA.
Thesagro:	Caprino Corynebacterium Pseudotuberculosis Linfadenite Caseosa Ovino
NAL Thesaurus:	caseous lymphadenitis
Keywords:	PCR CLA
DOI:	10.1099/jmm.0.46997-0
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPC)