Title:	Expression analysis of Pisum sativum putative defence genes during Orobanche crenata infection.
Authors:	DIE, J. V. ROMAN, B. SALVADOR, N. RODRIGUEZ, M. A. D. GONZÁLEZ-VERDEJO, C. I.
Affiliation:	José Vicente Die, IFAPA; Belén Román, IFAPA; Nadal Moyano Salvador, IFAPA; MIGUEL ANGEL DITA RODRIGUEZ, CNPMF; Clara Isabel González-Verdejo, IFAPA.
Date Issued:	2009
Citation:	Crop and Pasture Science, Victoria, v. 60, n. 5, p. 490-498, mai. 2009.
Description:	The root holoparasitic angiosperm Orobanche crenata is a severe constraint to the cultivation of legumes. Breeding for resistance is a difficult task. Understanding the mechanisms underlying host resistance is a fundamental issue for the genetic improvement of legumes. In this work, the temporal expression patterns of 8 defence-genes known to be involved in different metabolic pathways activated during several plant-pathogen interactions were investigated in Pisum sativum. Molecular analyses were carried out using quantitative real-time polymerase chain reaction during the initial stages of the parasitisation process in susceptible (Messire) and incompletely resistant (Ps624) pea genotypes. Transcriptional changes in response to O. crenata revealed induction of genes putatively encoding pathogenesis-related proteins, peroxidase activity, and dehydration stress-responsive signalling. This, combined with high constitutive gene expression mediating the phenylpropanoid pathway were observed as part of the defence mechanisms triggered in Ps624 to restrict the growth of the parasite.
Thesagro:	Melhoramento Genético Vegetal Patógeno
NAL Thesaurus:	parasitic plants
Keywords:	Plant defence Real-time PCR
ISSN:	1836-0947
DOI:	DOI: 10.1071/CP08274
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPMF)