Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/880373
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dc.contributor.authorMALUF, M. P.pt_BR
dc.contributor.authorSILVA, C. C. dapt_BR
dc.contributor.authorOLIVEIRA, M. de P. A. dept_BR
dc.contributor.authorTAVARES, A. G.pt_BR
dc.contributor.authorSILVAROLLA, M. B.pt_BR
dc.contributor.authorGUERREIRO FILHO, O.pt_BR
dc.date.accessioned2011-04-09T22:27:49Z-
dc.date.available2011-04-09T22:27:49Z-
dc.date.created2011-03-10pt_BR
dc.date.issued2009pt_BR
dc.identifier.citationGenetics and Molecular Biology, v. 34, n. 2, p. 802-810, 2009.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/880373pt_BR
dc.descriptionIn this work, we studied the biosynthesis of caffeine by examining the expression of genes involved in this biosynthetic pathway in coffee fruits containing normal or low levels of this substance. The amplification of gene-specific transcripts during fruit development revealed that low-caffeine fruits had a lower expression of the theobromine synthase and caffeine synthase genes and also contained an extra transcript of the caffeine synthase gene. This extra transcript contained only part of exon 1 and all of exon 3. The sequence of the mutant caffeine synthase gene revealed the substitution of isoleucine for valine in the enzyme active site that probably interfered with enzymatic activity. These findings indicate that the absence of caffeine in these mutants probably resulted from a combination of transcriptional regulation and the presence of mutations in the caffeine synthase amino acid sequence.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectNaturally decaffeinatedpt_BR
dc.subjectCoffeept_BR
dc.subjectCup qualitypt_BR
dc.subjectDifferential expressionpt_BR
dc.titleAltered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2011-04-10T11:11:11Zpt_BR
riaa.ainfo.id880373pt_BR
riaa.ainfo.lastupdate2011-03-10pt_BR
dc.contributor.institutionMIRIAN PEREZ MALUF, SAPC; CARLA CRISTINA DA SILVA, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; MICHELLE DE PAULA ABREU DE OLIVEIRA, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; ALINE GOMES TAVARES, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; MARIA BERNADETE SILVAROLLA, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; OLIVEIRO GUERREIRO FILHO, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'.pt_BR
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