Title:	Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica.
Authors:	MALUF, M. P. SILVA, C. C. da OLIVEIRA, M. de P. A. de TAVARES, A. G. SILVAROLLA, M. B. GUERREIRO FILHO, O.
Affiliation:	MIRIAN PEREZ MALUF, SAPC; CARLA CRISTINA DA SILVA, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; MICHELLE DE PAULA ABREU DE OLIVEIRA, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; ALINE GOMES TAVARES, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; MARIA BERNADETE SILVAROLLA, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'; OLIVEIRO GUERREIRO FILHO, INSTITUTO AGRONÔMICO, CENTRO DE CAFE 'ALCIDES CARVALHO'.
Date Issued:	2009
Citation:	Genetics and Molecular Biology, v. 34, n. 2, p. 802-810, 2009.
Description:	In this work, we studied the biosynthesis of caffeine by examining the expression of genes involved in this biosynthetic pathway in coffee fruits containing normal or low levels of this substance. The amplification of gene-specific transcripts during fruit development revealed that low-caffeine fruits had a lower expression of the theobromine synthase and caffeine synthase genes and also contained an extra transcript of the caffeine synthase gene. This extra transcript contained only part of exon 1 and all of exon 3. The sequence of the mutant caffeine synthase gene revealed the substitution of isoleucine for valine in the enzyme active site that probably interfered with enzymatic activity. These findings indicate that the absence of caffeine in these mutants probably resulted from a combination of transcriptional regulation and the presence of mutations in the caffeine synthase amino acid sequence.
Keywords:	Naturally decaffeinated Coffee Cup quality Differential expression
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (SAPC)