Título:	Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers.
Autoria:	MISSIO, R. F. CAIXETA, E. T. ZAMBOLIM, E. M. PENA, G. F. ZAMBOLIM, L. DIAS, L. A. S. SAKIYAMA, N. S.
Afiliação:	Universidade Federal do Paraná; EVELINE TEIXEIRA CAIXETA, SAPC; BioCafé; BioCafé; BioCafé; UFV; BioCafé/UFV.
Ano de publicação:	2011
Referência:	Genetics and Molecular Research, v. 10, n. 4, p 2366-2381, 2011.
Conteúdo:	Coffee is one of the main agrifood commodities traded worldwide. In 2009, coffee accounted for 6.1% of the value of Brazilian agricultural production, generating a revenue of US$6 billion. Despite the importance of coffee production in Brazil, it is supported by a narrow genetic base, with few accessions. Molecular differentiation and diversity of a coffee breeding program were assessed with gSSR and EST-SSR markers. The study comprised 24 coffee accessions according to their genetic origin: arabica accessions (six traditional genotypes of C. arabica), resistant arabica (six leaf rust-resistant C. arabica genotypes with introgression of Híbrido de Timor), robusta (five C. canephora genotypes), Híbrido de Timor (three C. arabica x C. canephora), triploids (three C. arabica x C. racemosa), and racemosa (one C. racemosa). Allele and polymorphism analysis, AMOVA, the Student t-test, Jaccard?s dissimilarity coefficient, cluster analysis, correlation of genetic distances, and discriminant analysis, were performed. EST-SSR markers gave 25 exclusive alleles per genetic group, while gSSR showed 47, which will be useful for differentiating accessions and for fingerprinting varieties. The gSSR markers detected a higher percentage of polymorphism among (35% higher on average) and within (42.9% higher on average) the genetic groups, compared to EST-SSR markers. The highest percentage of polymorphism within the genetic groups was found with gSSR markers for robusta (89.2%) and for resistant arabica (39.5%). It was possible to differentiate all genotypes including the arabica-related accessions. Nevertheless, combined use of gSSR and EST-SSR markers is recommended for coffee molecular characterization, because EST-SSRs can provide complementary information.
NAL Thesaurus:	Coffea Discriminant analysis
Palavras-chave:	Genetic diversity Microsatellite marker
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em anais de congresso (SAPC)