Title:	RAPD and ISSR markers in the evaluation of genetic divergence among accessions of elephant grass.
Authors:	LIMA, R. S. N. de DAHER, R. F. GONÇALVES, L. S. A. ROSSI, D. A. AMARAL JÚNIOR, A. T. do PEREIRA, M. G. LEDO, F. J. da S.
Affiliation:	R. S. N. DE LIMA, Universidade Estadual do Norte Fluminense Darcy Ribeiro; R. F. DAHER, Universidade Estadual do Norte Fluminense Darcy Ribeiro; L. S. A. GONÇALVES, Universidade Estadual do Norte Fluminense Darcy Ribeiro; D. A. ROSSI, Universidade Estadual do Norte Fluminense Darcy Ribeiro; A. T. DO AMARAL JÚNIOR, Universidade Estadual do Norte Fluminense Darcy Ribeiro; M. G. PEREIRA, Universidade Estadual do Norte Fluminense Darcy Ribeiro; FRANCISCO JOSE DA SILVA LEDO, CNPGL.
Date Issued:	2011
Citation:	Genetics and Molecular Research, v. 10, n. 3, p. 1214-1223, 2011.
Description:	Considering the expected genetic variability of elephant grass (Pennisetum purpureum), due to its cultivation in different continents, we characterized and estimated the genetic divergences between 46 accessions of elephant grass with different edaphoclimatic adaptations, using RAPD and ISSR markers. We evaluated, comparatively, the consistency of the information achieved with these markers. Twenty-six RAPD and 25 ISSR primers were employed. The RAPD markers produced 185 bands, 72% of which were polymorphic, with a mean of 5.11 polymorphic bands per primer. The 25 ISSR starters produced 216 bands; 76% were polymorphic, with a mean of 6.56 polymorphic bands per primer. The correlation between the genetic distances achieved by the RAPD and ISSR markers was 0.76, which is highly significant by the Mantel test. Based on UPGMA grouping, considering the point of sudden change, five and six groups were formed for the data from the RAPD and ISSR markers, respectively. These markers provided partially concordant groups, indicating that these techniques can provide consistent information and consequently could be used in studies of genetic diversity among accessions.
Keywords:	Pennisetum purpureum Schum RAPD and ISSR markers Genetic variability
DOI:	https://doi.org/10.4238/vol10-3gmr1107
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPGL)