Use este identificador para citar ou linkar para este item:
http://www.alice.cnptia.embrapa.br/alice/handle/doc/985516
Título: | Reproductive parameters and the use of MOET in transgenic founder goat carrying the human granulocyte colony-stimulating factor (hG-CSF) gene. |
Autoria: | MOURA, R. R. SOUZA-FABJAN, J. M. G. FONSECA, J. F. da MELO, C. H. S. SANCHEZ, D. J. D. VIEIRA, M. P. ALMEIDA, T. M. SEROVA, I. A. SEROV, O. L. PEREIRA, A. F. TEIXEIRA, D. I. A. MELO, L. M. FREITAS, V. J. F. |
Afiliação: | Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; JEFERSON FERREIRA DA FONSECA, CNPC; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; Institute of Cytology and Genetics, Novosibirsk, Russia.; Institute of Cytology and Genetics, Novosibirsk, Russia.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil. |
Ano de publicação: | 2014 |
Referência: | Animal Reproduction, v. 11, n. 1, p. 37-43, Jan./Mar. 2014. |
Conteúdo: | Abstract: This study aimed to monitor estrous cycle parameters of a human granulocyte colony-stimulating factor (hG-CSF)-transgenic founder female goat and to perform superovulation and embryo recovery (surgical or transcervical method) for further transfer to recipients to quickly obtain offspring. Two experiments were performed using a transgenic (TF) and a non-transgenic (NTF) female. In experiment 1, three estrous cycles were monitored for the following parameters: estrus behavior, progesterone concentration and ovarian activity. In experiment 2, two superovulation/embryo recovery sessions were performed and the recovered embryos were transferred to previously prepared recipients. Data were compared by either t test or Fisher's exact test. The mean interval between natural estrus was 20.7 ± 0.6 and 19.7 ± 0.6 (P > 0.05) days for the TF and NTF, respectively. Progesterone concentrations and ovarian activity were normal and similar between goats. The ovulation rate was similar between TF and NTF (12.0 ± 1.4 vs. 18.0 ± 4.2 CL; P > 0.05). No significant differences in embryo recovery rate (P > 0.05) were observed between the surgical and transcervical methods for TF (69.2 vs. 72.7%) or NTF (100.0 vs. 86.7%). Sixteen embryos from the TF were transferred to recipients, and eight kids were born. Among these kids, the transgene was identified in three (two males and one female), resulting in a transgenesis rate of 37.5%. In summary, the TF is a true founder, since she proved fertility and capacity of transmitting the hG-CSF transgene to progeny, suggesting that the analyzed reproductive traits were not compromised by the presence of the transgene. |
Thesagro: | Caprino Cabra Transferência de embrião Hormônio animal Progesterona Superovulação |
NAL Thesaurus: | Embryo transfer Progesterone Superovulation Transgenesis |
Palavras-chave: | Transgenia Goat |
Tipo do material: | Artigo de periódico |
Acesso: | openAccess |
Aparece nas coleções: | Artigo em periódico indexado (CNPC) |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
---|---|---|---|---|
APIReproductiveparameters.pdf | 421,97 kB | Adobe PDF | Visualizar/Abrir |