Title:	Serologies use in the identification of insects predators prey.
Authors:	REDOAN, A. C. M. SOUSA-SILVA, C. R. CRUZ, I.
Affiliation:	ANA CAROLINA MACIEL REDOAN, UFSCAR, São Carlos, SP.; CARLOS ROBERTO SOUSA-SILVA, UFSCAR, São Carlos, SP.; IVAN CRUZ, CNPMS.
Date Issued:	2016
Citation:	Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 15, n. 2, p. 157-170, 2016.
Description:	This study aimed to produce antiserum for the main pests found in maize, Spodoptera frugiperda, Helicoverpa zea and armigera, Rhopalosiphum maidis, and uses it to determine their predators. Pest samples were macerated in 0.85% saline solution. The macerated were centrifuged and the supernatants were used as immunizing antigens to obtain the antiserum. For this purpose, a rabbit was immunized with 3.0 ml of the immunizing antigen on the lymph node region. Homologous serological tests were performed by double diffusion in agar. The homologous serological reactions were positive after seven days of antigen inoculation. The technique has sensitivity to detect predation of the pests studied. The tests were positive for a prey in the digestive tract of the predator to 96 hours of ingestion. Regarding the number of prey ingested there was no difference in the lines. It is concluded that after a single injection of antigen in rabbit lymph node region, it was possible to get an antiserum specific for pests of corn. Field tests showed that there was a certain food preference of Doru luteipes by S. frugiperda, Chrysoperla externa by aphid.
Thesagro:	Zea mays Antígeno Antissoro
NAL Thesaurus:	Antigens Antiserum Predation
Keywords:	Predação
DOI:	10.18512/1980-6477/rbms.v15n2p157-170
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPMS)