Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/484159
Título: Identificação através de PCR dos genes Cryl de cepas de Bacillus thuringiensis Berliner eficientes contra a lagarta do cartucho, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae).
Autoria: VALICENTE, F. H.
BARRETO, M. R.
VASCONCELOS, M. J. V. de
FIGUEIREDO, J. E. F. de
PAIVA, E.
Afiliação: FERNANDO HERCOS VALICENTE, CNPMS; MARIA JOSE VILACA DE VASCONCELOS, CNPMS.
Ano de publicação: 2000
Referência: Anais da Sociedade Entomológica do Brasil, Jaboticabal, v. 29, n. 1, p. 147-153, 2000.
Conteúdo: Fall armyworm, Spodoptera frugiperda (J.E. Smith), is the most important maize insect pest in Brazil and its damage can reduce yield up to 34%. The objective of this work was to utilize the PCR technique to identify Bacillus thuringiensis (B.t) strains that control S. frugiperda in maize crops. Biossays showed that 16 strains were very effective against S. frugiperda, 15 of them collected from soil samples and one (T09) obtained at the Institute Pasteur. The DNA of these strains were probed with cryl general primers and their proteins were analyzed by SDS-PAGE eletrophoresis. All strains present positive results for cryl genes. To further characterize these strains, specific cryl primers were empolyed. PCR technique showed that some strains harbour the same cryl genes. The only difference was the amplification of an unexpected fragment of approximately 160bp when a mixture of cry1B and cry1D specific primers was used. Analysis by phase contrast microscope showed that crystal proteins produced by these strains were all bipyramidal crystals. Also, eltrophoretic analysis of proteins by SDS-PAGE showed the same protein banding pattern for most of the strains.
Thesagro: Controle Biológico
Spodoptera Frugiperda
NAL Thesaurus: Insecta
biological control
Palavras-chave: Lagarta do cartucho
Cry1
Tipo do material: Artigo de periódico
Acesso: openAccess
Aparece nas coleções:Artigo em periódico indexado (CNPMS)

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