Title:	Limited vegetative compatibility as a cause of somatic recombination in: Trichoderma pseudokoningii.
Authors:	BARCELLOS, F. G. HUNGRIA, M. PIZZIRANI-KLEINER, A. A.
Affiliation:	FERNANDO GOMES BARCELLOS, Universidade Paranaense - Umuarama, PR.; MARIANGELA HUNGRIA DA CUNHA, CNPSO; ALINE APARECIDA PIZZIRANI-KLEINER, USP - ESALQ.
Date Issued:	2011
Citation:	Brazilian Journal of Microbiology, São Paulo, v. 42, n. 4, p. 1625-1637, Oct./Dec. 2011.
Description:	With the aim of a better characterization of the somatic recombination process in Trichoderma pseudokoningii, a progeny from crossings between T. pseudokoningii strains contrasting for auxotroph markers was characterized by RAPD markers and PFGE (electrophoretic karyotype). Cytological studies of the conidia, conidiogenesis and heterokaryotic colonies were also performed. The genotypes of the majority of the recombinant strains analyzed were similar to only one of the parental strains and the low frequency of polymorphic RAPD bands suggested that the nuclear fusions may not occur into the heterokaryon. In some heterokaryotic regions the existence of intensely staining hyphae might be related to cell death. We proposed that a mechanism of somatic recombination other than parasexuality might occur, being related to limited vegetative compatibility after postfusion events, as described for other Trichoderma species.
Keywords:	Somatic recombination
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPSO)