Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1000338
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dc.contributor.authorVALENTE, A. M. M. P.pt_BR
dc.contributor.authorBOFFO, E. F.pt_BR
dc.contributor.authorMELO, I. S. dept_BR
dc.contributor.authorFERREIRA, A. G.pt_BR
dc.date.accessioned2014-11-17T11:11:11Zpt_BR
dc.date.available2014-11-17T11:11:11Zpt_BR
dc.date.created2014-11-17pt_BR
dc.date.issued2014pt_BR
dc.identifier.citationApplied Magnetic Resonance, Wien, v. 45, n. 3, p. 207-215, 2014.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1000338pt_BR
dc.descriptionAbstract: This study proposes a simple methodology to construct the production curves of mycophenolic acid by Penicillium sp. (CASP5) and citrinin by Penicillium sp. (CATL1.1) in the crude extract, without any purification. The quantification of the compounds was done by 1H nuclear magnetic resonance (NMR) using the signal integration and an internal standard, N,N-dimethylformamide. Fungi were cultivated for a period of 20 days and quantification of the metabolites in the extracts was done starting from time zero, 2 days and after this period in an interval of 4 days. The maximum production of mycophenolic acid and citrinin was obtained at 12 and 8 days of fermentation, respectively. These results show that the 1H NMR technique was efficient to define the production curves of mycophenolic acid and citrinin directly in the crude extracts. In addition, the technique made it possible to evaluate the purity degree of the substances obtained in the extraction process. Furthermore, this is the first study that uses the 1H NMR technique to determine the production curves of secondary metabolites.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectMycophenolic acidpt_BR
dc.subjectPenicillium sppt_BR
dc.subjectNMRpt_BR
dc.titleQuantification of mycophenolic acid and citrinin produced by Penicillium sp. using 1H NMR.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2014-11-17T11:11:11Zpt_BR
dc.subject.thesagroFungopt_BR
dc.subject.thesagroAnálise de laboratóriopt_BR
dc.subject.nalthesaurusCitrininpt_BR
dc.subject.nalthesaurusNuclear magnetic resonance spectroscopypt_BR
riaa.ainfo.id1000338pt_BR
riaa.ainfo.lastupdate2014-11-17pt_BR
dc.contributor.institutionANGELA MARIA MONTES PERAL VALENTE, UFSCar; ELISANGELA FABIANA BOFFO, UFBA; ITAMAR SOARES DE MELO, CNPMA; ANTONIO G FERREIRA, IFSCar.pt_BR
Appears in Collections:Artigo em periódico indexado (CNPMA)

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