Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1001427
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dc.contributor.authorBEVITORI, R.pt_BR
dc.contributor.authorOLIVEIRA, M. B.pt_BR
dc.contributor.authorGROSSI-DE-SÁ, M. F.pt_BR
dc.contributor.authorLANNA, A. C.pt_BR
dc.contributor.authorSILVEIRA, R. D. dapt_BR
dc.contributor.authorPETROFEZA, S.pt_BR
dc.date.accessioned2014-12-03T11:11:11Zpt_BR
dc.date.available2014-12-03T11:11:11Zpt_BR
dc.date.created2014-12-03pt_BR
dc.date.issued2014pt_BR
dc.identifier.citationGenetics and Molecular Research, v. 13, n. 4, p. 9795-9805, 2014.pt_BR
dc.identifier.issn1676-5680pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1001427pt_BR
dc.descriptionDrought and rice blast disease caused by Magnaporthe oryzae are two of the most serious threats to global rice production. To explore the mechanisms underlying gene expression induced in rice by stresses, studies involving transcriptome analyses have been conducted over the past few years. Thus, it is crucial to have a reliable set of reference genes to normalize the expression levels of rice genes affected by different stresses. To identify potential reference genes for studies of the differential expression of target genes in rice under M. oryzae infection and drought conditions, the present study evaluated five housekeeping genes for the normalization of gene expression. The stability of the expression of these genes was assessed using the analytical software packages geNorm and NormFinder. For all samples analyzed, the stability rank was UBQ5 > GAPDH > eIF-4α > β-TUB > 18S rRNA. The data showed that the UBQ5, GAPDH, and eIF-4α genes are appropriate, high-performing reference genes and will be highly useful in future expression studies of fungal infections and drought in rice.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleSelection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2014-12-03T11:11:11Zpt_BR
dc.subject.thesagroArrozpt_BR
dc.subject.thesagroOryza sativapt_BR
dc.subject.thesagroDoença de plantapt_BR
dc.subject.thesagroBrusonept_BR
dc.subject.thesagroGenética molecularpt_BR
dc.subject.thesagroSecapt_BR
dc.subject.nalthesaurusGene expressionpt_BR
dc.subject.nalthesaurusRicept_BR
dc.subject.nalthesaurusMagnaporthe oryzaept_BR
dc.subject.nalthesaurusBlast diseasept_BR
riaa.ainfo.id1001427pt_BR
riaa.ainfo.lastupdate2014-12-03pt_BR
dc.identifier.doihttp://dx.doi.org/10.4238/2014.November.27.7pt_BR
dc.contributor.institutionROSANGELA BEVITORI, CNPAF; MARILIA BARROS OLIVEIRA, doutoranda UFG; MARIA FATIMA GROSSI DE SA, CENARGEN; ANNA CRISTINA LANNA, CNPAF; RICARDO DIÓGENES DA SILVEIRA; SILVANA PETROFEZA, UFG.pt_BR
Appears in Collections:Artigo em periódico indexado (CNPAF)

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