Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1001497
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dc.contributor.authorNAKAYAMA, T. J.pt_BR
dc.contributor.authorRODRIGUES, F. A.pt_BR
dc.contributor.authorNEUMAIER, N.pt_BR
dc.contributor.authorMARCELINO-GUIMARÃES, F. C.pt_BR
dc.contributor.authorFARIAS, J. R. B.pt_BR
dc.contributor.authorOLIVEIRA, M. C. N. dept_BR
dc.contributor.authorBORÉM, A.pt_BR
dc.contributor.authorOLIVEIRA, A. C. B. dept_BR
dc.contributor.authorEMYGDIO, B. M.pt_BR
dc.contributor.authorNEPOMUCENO, A. L.pt_BR
dc.date.accessioned2014-12-04T08:14:25Z-
dc.date.available2014-12-04T08:14:25Z-
dc.date.created2014-12-03pt_BR
dc.date.issued2014pt_BR
dc.identifier.citationGenetics and Molecular Research, Ribeirão Preto, v. 13, n. 1, p. 860-871, 2014.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1001497pt_BR
dc.descriptionQuantitative real-time polymerase chain reaction (RT-qPCR) is a powerful tool used to measure gene expression. However, because of its high sensitivity, the method is strongly influenced by the quality and concentration of the template cDNA and by the amplification efficiency. Relative quantification is an effective strategy for correcting random and systematic errors by using the expression level of reference gene(s) to normalize the expression level of the genes of interest. To identify soybean reference genes for use in studies of flooding stress, we compared 5 candidate reference genes (CRGs) with the NormFinder and GeNorm programs to select the best internal control. The expression stability of the CRGs was evaluated in root tissues from soybean plants subjected to hypoxic conditions. Elongation factor 1-beta and actin-11 were identified as the most appropriate genes for RT-qPCR normalization by both the NormFinder and GeNorm analyses. The expression profiles of the genes for alcohol dehydrogenase 1, sucrose synthase 4, and ascorbate peroxidase 2 were analyzed by comparing different normalizing combinations (including no normalization) of the selected reference genes. Here, we have identified potential genes for use as references for RT-qPCR normalization in experiments with soybean roots growing in O2-depleted environments, such as flooding-stressed plants.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectEndogenous genespt_BR
dc.subjectFloodingpt_BR
dc.subjectHousekeeping genespt_BR
dc.subjectInternal control genespt_BR
dc.titleReference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2014-12-04T08:14:25Zpt_BR
dc.subject.nalthesaurusgene expressionpt_BR
riaa.ainfo.id1001497pt_BR
riaa.ainfo.lastupdate2014-12-03pt_BR
dc.identifier.doiDOI: 10.4238/2014.February.13.4pt_BR
dc.contributor.institutionUEL; CNPSo; NORMAN NEUMAIER, CNPSO; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; MARIA CRISTINA NEVES DE OLIVEIRA, CNPSO; UFV; ANA CLAUDIA BARNECHE DE OLIVEIRA, CPACT; BEATRIZ MARTI EMYGDIO, CPACT; ALEXANDRE LIMA NEPOMUCENO, SRI.pt_BR
Appears in Collections:Artigo em periódico indexado (CPACT)

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