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dc.contributor.authorCREVELIN, E. J.pt_BR
dc.contributor.authorCROTTI, A. E. M.pt_BR
dc.contributor.authorZUCCHI, T. D.pt_BR
dc.contributor.authorMELO, I. S. dept_BR
dc.contributor.authorMORAES, L. A. B.pt_BR
dc.date.accessioned2014-12-23T11:11:11Zpt_BR
dc.date.available2014-12-23T11:11:11Zpt_BR
dc.date.created2014-12-23pt_BR
dc.date.issued2014pt_BR
dc.identifier.citationJournal of Mass Spectrometry, Chichester, v. 49, n. 11, p. 1117-1126, 2014.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1003573pt_BR
dc.descriptionAbstracts: Actinomycetes, especially those belonging to the genus Streptomyces, are economically important from a biotechnological standpoint: they produce antibiotics, anticancer compounds and a variety of bioactive substances that are potentially applicable in the agrochemical and pharmaceutical industries. This paper combined accurate-mass electrospray tandem mass spectrometry in the full scan and product ion scan modes with compounds library data to identify the major compounds in the crude extract produced by Streptomyces sp. AMC 23; it also investigated how sodiated nonactin ([M + Na]+) fragmented. Most product ions resulted from elimination of 184 mass units due to consecutive McLafferty-type rearrangements. The data allowed identification of four macrotetrolides homologous to nonactin (monactin, isodinactin, isotrinactin/trinactin and tetranactin) as well as three related linear dimer compounds (nonactyl nonactoate, nonactyl homononactoate and homononactyl homononactoate). The major product ions of the sodiated molecules of these compounds also originated from elimination of 184 and 198 mass units. UPLC-MS/MS in the neutral loss scan mode helped to identify these compounds on the basis of the elimination of 184 and 198 mass units. This method aided monitoring of the relative production of these compounds for 32 days and revealed that the biosynthetic process began with increased production of linear dimers as compared with macrotetrolides. These data could facilitate dereplication and identification of these compounds in other microbial crude extracts.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectMacrotetrolidespt_BR
dc.subjectActinomycetespt_BR
dc.subjectUPLC-MS/MSpt_BR
dc.subjectNonactic acidpt_BR
dc.titleDereplication of Streptomyces sp. AMC 23 polyether ionophore antibiotics by accurate-mass electrospray tandem mass spectrometry.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2014-12-23T11:11:11Zpt_BR
dc.subject.thesagroActinomicetopt_BR
dc.subject.thesagroEspectrometriapt_BR
dc.subject.nalthesaurusStreptomycespt_BR
dc.subject.nalthesaurusActinomycetalespt_BR
dc.subject.nalthesaurusDNA fragmentationpt_BR
dc.subject.nalthesaurusExtractspt_BR
dc.subject.nalthesaurusUltra-performance liquid chromatographypt_BR
dc.subject.nalthesaurusTandem mass spectrometrypt_BR
riaa.ainfo.id1003573pt_BR
riaa.ainfo.lastupdate2014-12-23pt_BR
dc.contributor.institutionEDUARDO JOSE CREVELIN, FFCLRP-USP; ANTONIO EDUARDO MILLER CROTTI, FFCLRP-USP; TIAGO DOMINGUES ZUCCHI, FAPESP; ITAMAR SOARES DE MELO, CNPMA; LUIZ ALBERTO BERALDO MORAES, FFCLRP-USP.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CNPMA)

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